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pXPR_003_sgTFAP4 guide 2

Plasmid

#193587

Purpose

TFAP4 knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgTFAP4 guide 2 (TFAP4 Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPR3_BC3

Plasmid

#191858

Purpose

Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GCATGG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

Depositor

Kimberly Reynolds

Article

Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pGEM-T-Easy-HySp5-557

Plasmid

#193000

Purpose

Plasmid to generate an ISH probe for Hydra Sp5

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Hydra Sp5

Use

Cloning vector

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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ZfSp5a-377

Plasmid

#192997

Purpose

Plasmid that encodes for Zebrafish Sp5

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Zebrafish Sp5

Tags

HA

Expression

Mammalian

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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HySp5-227

Plasmid

#192996

Purpose

Plasmid that encodes for Hydra Sp5, lacking the SP box and DNA binding domain

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Hydra Sp5

Tags

HA

Expression

Mammalian

Mutation

no SP box and DNA binding domain

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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HySp5:Luc

Plasmid

#192985

Purpose

Plasmid to test the Hydra Sp5 promoter activity

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Hydra Sp5 promoter

Expression

Mammalian

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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ZfSp5l1

Plasmid

#192999

Purpose

Plasmid that encodes for Zebrafish Sp5-like

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Zebrafish Sp5-like

Tags

HA

Expression

Mammalian

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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HySp5-337

Plasmid

#192994

Purpose

Plasmid that encodes for Hydra Sp5, lacking the DNA binding domain

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Hydra Sp5

Tags

HA

Expression

Mammalian

Mutation

no DNA binding domain

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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ZfWnt3:Luc

Plasmid

#192992

Purpose

Plasmid to test the Zebrafish Wnt3 promoter activity

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Zebrafish Wnt3 promoter

Expression

Mammalian

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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Gly-tRNA-gRNA scaffold for library 2

Plasmid

#192365

Purpose

tRNA sequence provider (for checkpoint library)

Depositor

Lijia Ma

Article

Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

Insert

Gly tRNA-gRNA scaffold

Use

For golden gate assembly

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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Pro-tRNA-gRNA scaffold for library 2

Plasmid

#192366

Purpose

tRNA sequence provider (for checkpoint library)

Depositor

Lijia Ma

Article

Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

Insert

Pro tRNA-gRNA scaffold

Use

For golden gate assembly

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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Gln-tRNA-gRNA scaffold for library 1&2

Plasmid

#192367

Purpose

tRNA sequence provider (for checkpoint and immune library)

Depositor

Lijia Ma

Article

Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

Insert

Gln tRNA-gRNA scaffold

Use

For golden gate assembly

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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Gly-tRNA-gRNA scaffold for library 1

Plasmid

#192368

Purpose

tRNA sequence provider (for immune library)

Depositor

Lijia Ma

Article

Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

Insert

Gly tRNA-gRNA scaffold

Use

For golden gate assembly

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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Pro-tRNA-gRNA scaffold for library 1

Plasmid

#192369

Purpose

tRNA sequence provider (for immune library)

Depositor

Lijia Ma

Article

Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

Insert

Pro tRNA-gRNA scaffold

Use

For golden gate assembly

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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DpbCas12e

Plasmid

#188495

Purpose

Expresses FLAG tagged DpbCas12e

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

Cas12e

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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PlmCas12e

Plasmid

#188496

Purpose

Expresses FLAG tagged PlmCas12e

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

Cas12e

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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DpbdCas12e KRAB

Plasmid

#188505

Purpose

Expresses FLAG tagged DpbdCas12e KRAB

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12e

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D672A/E769A/D935A

Promoter

EF1a

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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Cas12j3

Plasmid

#188497

Purpose

Expresses FLAG tagged Cas12j3

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

Cas12j3

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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p-preSufI(IAC)

Plasmid

#168516

Purpose

Modified form of pre-SufI in pET25b. With C-terminal HHHHHHC (6xHisC) extension and mutations C17I and C295A.

Depositor

Siegfried Musser

Article

Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

Insert

pre-SufI(IAC)

Tags

6xHisC tag and HSV tag

Expression

Bacterial

Mutation

C17I and C295A

Promoter

T7

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_VPR_mcherry

Plasmid

#167894

Purpose

PiggyBac compatible plasmid expressing Cas9-VPR

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9-VPR

Use

CRISPR

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only