We narrowed to 7,692 results for: Lif;
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Plasmid#172825PurposeMammalian expression of a sgRNA targeting the intron 1 position 1 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330 _HITI donor B6
Plasmid#172847PurposeDRS-2 sgRNA expression under a U6 promotor and HITI donor B6 (Zhong et al, eLife 2021), mEGFP translational phase (1-1), excised by DRS-2 sgRNA (with SpCas9).DepositorInsertDRS-2 sgRNA and HITI donor (phase 1) encoding mEGFP
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUASTLOTattB_mCherry-CD8-vhhGFP
Plasmid#163930PurposeExpression of Morphotrap-intracellular (Grab-FP-intracellular) under control of UAS or LOP enhancerDepositorInsertFusion of vhhGPF4 nanobody (intracellular) with mouse CD8 transmembrane protein and mCherry fluorophor
UseCre/LoxTagsmCherryExpressionInsectAvailable SinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
PRK5-FLAG-USP13/5-4
Plasmid#61746Purposeexpress N-terminally FLAG tagged human chimera USP13/5-4DepositorTagsFLAGExpressionMammalianMutationUSP5 (1-716aa) fused to USP13 (722-864aa)PromoterCMVAvailable SinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
PRK5-FLAG-USP13/5-3
Plasmid#61745Purposeexpress N-terminally FLAG tagged human chimera USP13/5-3DepositorTagsFLAGExpressionMammalianMutationUSP5 (1-628aa) fused to USP13 (653-864aa)PromoterCMVAvailable SinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
PRK5-FLAG-USP13/5-1
Plasmid#61743Purposeexpress N-terminally FLAG tagged human chimera USP13/5-1DepositorTagsFLAGExpressionMammalianMutationUSP5 (1-185aa) fused to USP13 (197-864aa)PromoterCMVAvailable SinceFeb. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX330_rActb sgRNA / hSpCas9
Plasmid#172833PurposeMammalian expression of a sgRNA targeting the intron 1of rat Actb (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 1 of rActb under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_Tuba1b sgRNA / hSpCas9
Plasmid#172835PurposeMammalian expression of a sgRNA targeting the intron 1 of Tuba1b (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 1 of Tuba1b under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-OSF-TRIM5alpha(human)(R332P)
Plasmid#79037PurposeBaculoviral entry vector to produce Bac-to-bac baculovirusesDepositorInsertOSF-TRIM5alpha(human R332P) (TRIM5 Human)
TagsOneSTrEP-FLAGExpressionInsectMutationChanged Arg 332 to ProPromoterpolyhedrinAvailable SinceMarch 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX330_rSyn1 sgRNA / hSpCas9
Plasmid#172840PurposeMammalian expression of a sgRNA targeting the intron 21 (last intron) of rSyn1 (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 21 of rSyn1 under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only