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pMCh-695

Plasmid

#92263

Purpose

Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 with mutated PAM2 motif in D. melanogaster cells

Depositor

Marina Chekulaeva, Witold Filipowicz

Article

Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

Insert

dGW182 PAM2 mut (gw Fly)

Tags

NHA

Expression

Insect

Mutation

The following point mutations were introduced: EF…

Promoter

Ac5

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pMT-EGFP-Actin5c

Plasmid

#15312

Depositor

Ron Vale

Article

Rogers et al J Cell Biol. 2003 Sep 15. 162(6):1079-88

Insert

Actin5c (Act5C Fly)

Tags

EGFP and His

Expression

Insect

Available Since

Oct. 12, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(-)-V5.mER.hOr47a

Plasmid

#126473

Purpose

Human codon-optimized version of D. melanogaster Or47a (hOr47a) with an N-terminal tag derived from human HCN1 (mER) for improved intracellular trafficking in mammalian cells

Depositor

Bill Hansson

Article

Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

Insert

Or47a (Or47a Fly)

Tags

H. sapiens HCN1 106VNKFSL111 (mER) and V5 tag

Expression

Mammalian

Mutation

codon optimization for H. sapiens

Promoter

CMV

Available Since

June 13, 2019

Availability

Academic Institutions and Nonprofits only

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KHBD00210

Plasmid

#34361

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG17894 (cnc Fly)

Use

Gateway Cloning

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00009

Plasmid

#34172

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG5058 (grh Fly)

Use

Gateway Cloning

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-N1 Btsz2

Plasmid

#40068

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

Btsz2 (btsz Fly)

Tags

EGFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Oct. 26, 2012

Availability

Academic Institutions and Nonprofits only
pUAS-CG6867-sfGFP

Plasmid

#240237

Purpose

Expression of CG6867-sfGFP under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al Nat Commun. 2026 Apr 20. doi: 10.1038/s4

Insert

CG6867 (CG6867 Fly)

Tags

sfGFP

Expression

Insect

Promoter

UAS

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR_hhN-HA

Plasmid

#240225

Purpose

Gateway entry clone with N-terminal hedgehog (hhN) tagged with HA (contains stop codon)

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al Nat Commun. 2026 Apr 20. doi: 10.1038/s4

Insert

hedgehog N-terminal fragment (hh Fly)

Use

Gateway shuttle vector

Tags

HA-spGFP11

Mutation

aa 1-257

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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Hy_U5:34A eGFP SV40

Plasmid

#195064

Purpose

Expresses eGFP when there is readthrough transcription from the Drosophila U5:34A gene

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

snRNA:U5:34A (snRNA:U5:34A Fly)

Expression

Insect

Promoter

U5:34A

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1_1-1717_X

Plasmid

#147982

Purpose

Insect Expression of DmNot1_1-1717

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1_1-1717 (Not1 Fly)

Expression

Insect

Mutation

6 silent mutations and two non silent N605S, K759…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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