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2COX8-BmTyr

Plasmid

#222943

Purpose

mitochondrion-localized BmTyr

Depositor

Itaru Hamachi

Article

Zhu et al J Am Chem Soc. 2024 Mar 20;146(11):7515-

Insert

2COX8-BmTyr-Flag

Tags

Flag tag

Expression

Mammalian

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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IgK-BmTyr-KDEL

Plasmid

#222942

Purpose

ER-localized BmTyr

Depositor

Itaru Hamachi

Article

Zhu et al J Am Chem Soc. 2024 Mar 20;146(11):7515-

Insert

IgK-BmTyr-Flag-KDEL

Tags

Flag tag

Expression

Mammalian

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pET22b-Empty-cleavableELP

Plasmid

#219401

Purpose

Insert protease recognition site with BsaI to make custom protease-responsive elastin-like polypeptide (ELP)

Depositor

Danielle Mai

Article

Wirtz et al Biomacromolecules. 2024 Jul 9. doi: 10.1

Insert

Empty-cleavableELP

Tags

6xHis

Expression

Bacterial

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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T7-LacO-TetR_p15A

Plasmid

#171664

Purpose

Expression of TetR in BL21(DE3) E. coli. Contains p15A origin of replication.

Depositor

Tara Deans

Article

MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

Insert

T7-LacO-TetR-T7 Stop

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pOC5 cloning template vector

Plasmid

#183424

Purpose

FlpOFF knock-in vector

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Type

Empty backbone

Use

CRISPR

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pSABAD14-142

Plasmid

#61825

Purpose

TvoVMAC16 intein

Depositor

Hideo Iwai

Article

Aranko et al Mol Biosyst. 2014 May;10(5):1023-34. doi

Insert

TvoVMAC16 intein

Tags

GB1-H6

Expression

Bacterial

Promoter

pBAD

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pHYBAD177

Plasmid

#61826

Purpose

TvoVMAC7 intein

Depositor

Hideo Iwai

Article

Aranko et al Mol Biosyst. 2014 May;10(5):1023-34. doi

Insert

TvoVMAC16 intein

Tags

GB1-H6

Expression

Bacterial

Promoter

pBAD

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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2C::tdTomato Reporter

Plasmid

#40281

Depositor

Samuel Pfaff

Article

Macfarlan et al Nature. 2012 Jul 5;487(7405):57-63.

Insert

2C Promoter (2-cell stage promoter)

Tags

tdTomato

Expression

Mammalian

Available Since

Sept. 26, 2012

Availability

Academic Institutions and Nonprofits only

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pMBP-MS2

Plasmid

#65104

Purpose

can be used to isolate RNA binding complexes

Depositor

Josep Vilardell

Article

Macias et al Mol Cell. 2008 Jun 20;30(6):732-42. doi:

Insert

MS2

Tags

MBP

Expression

Bacterial

Mutation

double mutation (V75Q and A81G) that prevents oli…

Promoter

tac

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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V48 ELP

Plasmid

#68395

Purpose

Elastin-like polypeptide (VPGxG) with 48 repeats of valine guest residue

Depositor

Andrew MacKay

Article

Shah et al Protein Sci. 2012 Jun;21(6):743-50. doi:

Insert

V48 ELP

Expression

Bacterial

Promoter

T7

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pMP71-BFP

Plasmid

#141355

Purpose

pMP71 expression vector containing BFP

Depositor

Ton Schumacher

Article

Bresser et al Commun Biol. 2020 May 29;3(1):273. doi:

Insert

tagBFP

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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BmTyr-Flag

Plasmid

#220779

Purpose

non-targeted BmTyr throughout the cytoplasm

Depositor

Itaru Hamachi

Article

Zhu et al J Am Chem Soc. 2024 Mar 20;146(11):7515-

Insert

Bacillus megaterium Tyrosinase

Tags

Flag tag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pFUGW NLS-FlpO

Plasmid

#183448

Purpose

Lentiviral FlpO vector

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

NLS-FlpO

Use

Lentiviral

Expression

Mammalian

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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QUAS-5-T7-GFP_ColE1

Plasmid

#171653

Purpose

QUAS five base pairs upstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

Depositor

Tara Deans

Article

MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

Insert

QUAS-5-T7

Tags

ssrA degradation tag (DAS+4)

Expression

Bacterial

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-M45HA

Plasmid

#17573

Depositor

Wolfram Brune

Article

Mack et al Proc Natl Acad Sci U S A. 2008 Feb 26. 1

Insert

murine cytomegalovirus M45 gene

Tags

HA

Expression

Mammalian

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only

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pMP71-Kaede

Plasmid

#141352

Purpose

pMP71 expression vector containing Kaede

Depositor

Ton Schumacher

Article

Bresser et al Commun Biol. 2020 May 29;3(1):273. doi:

Insert

Kaede

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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VV001: WT Arch-3 in pET28b

Plasmid

#58487

Purpose

Expresses WT Archaerhodopsin-3 (with 6x C-terminal His tag) in E. coli

Depositor

Adam Cohen

Article

Maclaurin et al Proc Natl Acad Sci U S A. 2013 Apr 9;110

Insert

Archaerhodopsin-3 (with C-terminal His tag)

Expression

Bacterial

Promoter

T7

Available Since

July 23, 2014

Availability

Academic Institutions and Nonprofits only

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pBN396

Plasmid

#109326

Purpose

Expresses FLP enzyme and fluorescent mNeonGreen in the C. elegans germ line

Depositor

Peter Askjaer

Article

Macias-Leon et al MicroPubl Biol. 2018 Mar 19;2018:10.1791

Insert

mex-5 (mex-5 Nematode)

Expression

Worm

Promoter

mex-5

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pMP71-Katushka

Plasmid

#141351

Purpose

pMP71 expression vector containing Katushka

Depositor

Ton Schumacher

Article

Bresser et al Commun Biol. 2020 May 29;3(1):273. doi:

Insert

Katushka

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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pMP71-AzamiGreen

Plasmid

#141353

Purpose

pMP71 expression vector containing AzamiGreen

Depositor

Ton Schumacher

Article

Bresser et al Commun Biol. 2020 May 29;3(1):273. doi:

Insert

AzamiGreen

Use

Retroviral

Expression

Mammalian

Available Since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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