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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 8,831 results for: Pol

Showing: 901 - 920 of 8831 results

  4. pCD/NL-BH*deltavpu/RT-

    Plasmid
    #136985
    Purpose
    Second-generation lentiviral packaging plasmid lacking reverse transcriptase activity
    Depositor
    Jakob Reiser
    Article
    Zhang et al J Virol. 2004 Feb . 78(3):1219-29.
    Insert
    HIV-1 Gag, Pol (Integrase), Tat, Rev, Vpr, Vif
    Use
    Tags
    Expression
    Mammalian
    Mutation
    carries D110E mutation in the reverse transcripta…
    Promoter
    Available since
    Feb. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    136985-plasmid-map-sequence-id-271274

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  5. pFTK087

    Plasmid
    #171359
    Purpose
    Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.
    Depositor
    Arnold Driessen
    Article
    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021
    Insert
    P-ANtRNA[Pro1]-sgRNA-dummy-Esp3I, Pol-III sgRNA-plug-in transcription unit
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    n/a
    Promoter
    Available since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    171359-plasmid-map-sequence-id-351785

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  6. pLKO.1-blast shGAC

    Plasmid
    #110410
    Purpose
    shGAC (Target CCTCTGTTCTGTCAGAGTT), silence glutaminase isoform GAC, blasticidin selection.
    Depositor
    Sandra Martha Gomes Dias
    Insert
    GLS glutaminase (GLS Human)
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 (RNA Pol III)
    Available since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    110410-plasmid-map-sequence-id-218291

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  7. p33Fn

    Plasmid
    #158155
    Purpose
    Expression of crRNA array and FnCas12a using one Pol II promoter. For plant genome editing.
    Depositor
    Kabin Xie
    Article
    Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Rice codon optimized
    Promoter
    Available since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    158155-plasmid-map-sequence-id-321549

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  8. pcDNA3-CTE-Luc

    Plasmid
    #60881
    Purpose
    Reporter construct to study unspliced RNA export. Coding sequence of luciferase gene was sandwiched between 5' and 3' splice site sequences originating from the HIV gag/pol gene followed by CTE
    Depositor
    Vinay Nandicoori
    Article
    Rajanala et al PLoS One. 2012;7(1):e29921. doi: 10.1371
    Insert
    Luc-CTE
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    60881-plasmid-map-sequence-id-98231

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  9. pFTK086

    Plasmid
    #171358
    Purpose
    Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.
    Depositor
    Arnold Driessen
    Article
    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021
    Insert
    P-ANtRNA[Arg21]-sgRNA-dummy-Esp3I, Pol-III sgRNA-plug-in transcription unit
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    n/a
    Promoter
    Available since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    171358-plasmid-map-sequence-id-351696

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  12. pcpBpaRS

    Plasmid
    #196484
    Purpose
    To express benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into benzoylphenylalanine.
    Depositor
    Kensaku Sakamoto
    Article
    Hino et al Methods Mol Biol. 2012;794:215-28. doi:
    Insert
    benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS), amber suppressor tRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV (synthetase), Pol III tRNA promoter (tRNA)
    Available since
    Jan. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    196484-plasmid-map-sequence-id-388864

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  13. pCP-tRNA

    Plasmid
    #133812
    Purpose
    CRISPR-Cas9 system for genetic manipulation of Candida parapsilosis, C. orthopsilosis, and C. metapsilosis
    Depositor
    Geraldine Butler
    Article
    Lombardi et al mSphere. 2019 Mar 13;4(2). pii: 4/2/e001
    Insert
    cassette for the expression of the sgRNA from the C. parapsilosis RNA pol II GAPDH promoter
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    133812-plasmid-map-sequence-id-263618

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  17. pSMOX-mNG::LacI-V3

    Plasmid
    #210224
    Purpose
    Integrates a T7 RNA polymerase, tetracycline repressor and an mNeongreen Lac repressor fusion protein with a puromycin drug selection marker into the tubulin locus.
    Depositor
    Calvin Tiengwe
    Article
    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115
    Insert
    mNeongreen Lac repressor fusion protein
    Use
    Trypanosoma brucei expression
    Tags
    Expression
    Mutation
    Promoter
    Pol II readthrough transcription after integration
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    210224-plasmid-map-sequence-id-415797

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Showing: 901 - 920 of 8831 results