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Plasmid#117911PurposePlasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 6.5 uMDepositorInsertSNAP_Pro30_[cpNLuc23_24]eDHFR(R44A-H45Q)
UseTagsHis-tag and Strep-tagExpressionBacterialMutationR44A & H45Q on eDHFRPromoterT7Available sinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
gamma2 CR shRNA (shRNA2)
Plasmid#120794PurposeshRNA targeting the coding region of the gamma2 mRNADepositorInsertGABRG2 shRNA (Gabrg2 Rat)
UseRNAiTagsExpressionMutationPromoterAvailable sinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
JM3 [JBEI-6891]
Plasmid#89788PurposeBroad host range vector pBBR1-MCS2 containing 'tesA (E. coli), fadB (E. coli), Mlut_11700 (M. luteus) and fadM (E. coli) under control of BAD promoterDepositorInserttesA (E. coli), fadB (E. coli), Mlut_11700 (M. luteus) and fadM (E. coli)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
SPOP-V5 (BACK))
Plasmid#169722PurposeMammalian Expression of mouse SPOP Y353EDepositorInsertSPOP (Spop Mouse)
UseTagsV5ExpressionMammalianMutationY353EPromoterCMVAvailable sinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)-NADPH-LUCID-3 (C50 1.4 uM)
Plasmid#117910PurposePlasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 1.4 uMDepositorInsertSNAP_Pro30_[cpNLuc23_24]eDHFR(R44A)
UseTagsHis-tag and Strep-tagExpressionBacterialMutationR44A on eDHFRPromoterT7Available sinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)-NADPH-LUCID-2 (C50 29 nM)
Plasmid#117909PurposePlasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 29 nMDepositorInsertSNAP_Pro30_[cpNLuc23_24]eDHFR(R98A)
UseTagsHis-tag and Strep-tagExpressionBacterialMutationR98A on eDHFRPromoterT7Available sinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAPi
Plasmid#127548PurposeEmpty control for pGAPi-based RNAi silencing in tandem with APT transcript segmentDepositorInsertAdenine Phosphorybosyltransferase transcript segment
UseRNAi; Empty controlTagsExpressionMutationPromoterUbiquitinAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
MyoUTipGAPi
Plasmid#127549PurposePositive RNAi control for pGAPi-based RNAi silencing in tandem with APT transcript segmentDepositorInsertMyosin XIa+b 5'UTR and Adenine Phosphorybosyltransferase transcript segment
UseRNAi; Positive controlTagsExpressionMutationPromoterUbiquitinAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJL1-aadA
Plasmid#117050PurposeIn vitro expression of aadA (streptomycin resistance gene) from the T7 promoterDepositorInsertaadA
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFIN-IRBP156-tdTOM
Plasmid#44356DepositorInsertIRBP156-tdTOM
UseLentiviralTagsExpressionMutationPromotermurine interphotoreceptor retinoid-binding protei…Available sinceMay 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pFastBac-His-Flag-Tipin-L195A
Plasmid#23120DepositorInsertTipin (TIPIN Human)
UseTagsFlag- and HisExpressionInsectMutationChanged Leucine 195 to AlaninePromoterAvailable sinceMarch 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-His-Tipin-L195A
Plasmid#23121DepositorInsertTipin (TIPIN Human)
UseTagsHisExpressionInsectMutationChanged Leucine 195 to AlaninePromoterAvailable sinceMarch 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pYihI-N-term D-N/E-Q
Plasmid#233095PurposeExpression of GST-YihI with N-term aspartic acids (D) mutated to asparagine (N) and glutamic acids (E) mutated to glutamine (Q)DepositorInsertYihI with N-term aspartic acids (D) mutated to asparagine (N) and glutamic acids (E) mutated to glutamine (Q)
UseTagsGSTExpressionBacterialMutationN-term aspartic acids (D) mutated to asparagine (…PromoterAvailable sinceMay 7, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYihI-N-term D-A/E-L
Plasmid#233097PurposeExpression of GST-YihI with N-term aspartic acids (D) mutated to alanine (A) and glutamic acids (E) mutated to leucine (L)DepositorInsertYihI with N-term aspartic acids (D) mutated to alanine (A) and glutamic acids (E) mutated to leucine (L)
UseTagsGSTExpressionBacterialMutationN-term aspartic acids (D) mutated to alanine (A) …PromoterAvailable sinceMay 7, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAPH07
Plasmid#234345PurposeLibrary-scale IPTG-inducible gRNA expression for Type II dCas9 in Synechococcus sp. PCC 7002, genomically integrated next to glpKDepositorInsertType II dCas9 sgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterlacUV5Available sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPH021
Plasmid#234346PurposeLibrary-scale IPTG-inducible single transcript dual-gRNA expression for Type II dCas9 in Synechococcus sp. PCC 7002, genomically integrated next to glpKDepositorInsertsType II dCas9 sgRNA
Type II dCas9 sgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterlacUV5Available sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
paoB-CBE
Plasmid#220373Purposefor hA3A-nCas9 expression in Aspergillus oryzaeDepositorInserthA3A-nCas9
UseCRISPRTagsExpressionMutationPromoterPtef1Available sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
LacI pm
Plasmid#218097PurposePhagemid that expresses LacI but no phage proteins, used as a controlDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only