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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 165,214 results for: addgene

Showing: 901 - 920 of 165214 results

  1. pPyCAG-Nanog(Y119A)-IP

    Plasmid
    #62991
    Purpose
    Expresses Mouse Nanog Y119A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog Y119A Mutant (Nanog Mouse)
    Expression
    Mammalian
    Mutation
    Changed Tyrosine 119 (120 AA in Mouse Protein) to…
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62991-plasmid-map-sequence-id-104440

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  2. pZC12 (pFA6a-PSc-6xGly-V5-KanMX)

    Plasmid
    #44091
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, KanM…
    Available Since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44091-plasmid-map-sequence-id-61695

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  4. TDRD5

    Plasmid
    #32869
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    TDRD5 (TDRD5 Human)
    Tags
    His tag (with TEV cleavage)
    Expression
    Bacterial
    Available Since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  5. pCMV-ABE8.8m-WT-P2A-EGFP (BKS953)

    Plasmid
    #242651
    Purpose
    CMV promoter expression plasmid for human codon optimized ABE8.8m A-to-G base editor with SpCas9(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Insert
    pCMV-ABE8.8m-SpCas9-P2A-EGFP
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    ABE8.8 mutations in TadA
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242651-plasmid-map-sequence-id-489040

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  6. pPB-PGK-mCherry.OVA(323-339)

    Plasmid
    #190005
    Purpose
    Piggybac construct for constitutive expression of mCherry and Chicken Ovalbumin OVA323-339 peptide fusion
    Depositor
    Siyuan Zhang
    Insert
    mCherry.OVA(323-339)
    Use
    Synthetic Biology; Piggybac
    Tags
    OVA(323-339)
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    July 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    190005-plasmid-map-sequence-id-382848

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  8. pET15bHis6-pG-Tnp

    Plasmid
    #124884
    Purpose
    To transform BL21-Gold(DE3) and induced by IPTG for His-proteinG-Tnp (hyperactive mutant) fusion protein.
    Depositor
    Keji Zhao
    Insert
    protein G's C1D1C2D2C3 domains contained in the protein G gBLOCK.
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    124884-plasmid-map-sequence-id-243752

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  9. pEN_TT DNM1L-V5

    Plasmid
    #220359
    Purpose
    Gateway entry vector for an inducible V5-tagged DNM1L
    Depositor
    Kevin Janes
    Insert
    DNM1L (DNM1L Human)
    Use
    Entry vector for gateway cloning
    Tags
    V5
    Available Since
    Oct. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    220359-plasmid-map-sequence-id-447351

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  10. pAS_MmaPylT_EF1_NES-MmaPylRS WT

    Plasmid
    #174901
    Purpose
    expression of Mma PylRS with nuclear export sequence for amber suppression in mammalian cells; transient or piggy bac mediated integration
    Depositor
    Simon Elsaesser
    Insert
    Mma PylRS (pylS Methanosarcina mazei)
    Tags
    FLAG and NES
    Expression
    Mammalian
    Promoter
    EF1
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    174901-plasmid-map-sequence-id-348003

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  11. pAIO-Ef1a-PE2-GFP:KCNQ2-C201R_pp

    Plasmid
    #185061
    Purpose
    Ef1a driven PE2 with pegRNA for editing C201R mutation (including silent PAM mutation) in KCNQ2. See Addgene plasmid #184445
    Depositor
    Bobby Koeleman
    Insert
    U6:pegRNA:scaffold:PBS+RT template
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Nov. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    185061-plasmid-map-sequence-id-376807

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  12. pCW57.1-GFP_mmu-miR-292b-3p_sponge

    Plasmid
    #132622
    Purpose
    Expresses a TurboGFP transcript with test microRNA sponge for mmu-miR-292b-3p as 3'UTR; serves as template for other microRNA sponges
    Depositor
    Constance Ciaudo
    Insert
    stop codon, mmu-miR-292b-3p sponge sequence and polyA signal
    Use
    Lentiviral; Doxycycline inducible
    Expression
    Mammalian
    Promoter
    TRE
    Available Since
    Aug. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    132622-plasmid-map-sequence-id-259572

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  13. pLV V5-Eva1b

    Plasmid
    #175158
    Purpose
    Lentiviral expression of V5-tagged mouse Eva1b
    Depositor
    Larry Gerace
    Insert
    Eva1b (Eva1b Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Available Since
    Jan. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    175158-plasmid-map-sequence-id-352269

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  14. pLV Cenpv-V5

    Plasmid
    #175125
    Purpose
    Lentiviral expression of mouse Cenpv-V5
    Depositor
    Larry Gerace
    Insert
    Cenpv (Cenpv Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Available Since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    175125-plasmid-map-sequence-id-351968

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  15. XLone-BSD NFIL3 T2A Puro

    Plasmid
    #140028
    Purpose
    Tunable and temporal expression control of NFIL3 and puro resistant gene
    Depositor
    Xiaoping Bao
    Insert
    NFIL3 (NFIL3 Synthetic, Human)
    Use
    Synthetic Biology
    Tags
    puromycin
    Expression
    Mammalian
    Promoter
    TRE3G
    Available Since
    May 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    140028-plasmid-map-sequence-id-275842

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  17. pARID4B.1.0-gDNA

    Plasmid
    #132436
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    ARID4B (ARID4B Human)
    Use
    CRISPR
    Available Since
    July 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    132436-plasmid-map-sequence-id-266218

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  18. pDK365

    Plasmid
    #134195
    Purpose
    Retroviral plasmid with SKAP insert
    Depositor
    Iain Cheeseman
    Insert
    SKAP Alternate Start- LAP (with G5 hardeneded) (KNSTRN Human)
    Use
    Retroviral
    Tags
    LAP (GFP)
    Expression
    Mammalian
    Mutation
    Alternate Start, G5 siRNA hardened
    Available Since
    Feb. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    134195-plasmid-map-sequence-id-272443

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  19. pCSRNP3.1.0-gDNA

    Plasmid
    #132472
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    CSRNP3 (CSRNP3 Human)
    Use
    CRISPR
    Available Since
    Dec. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    132472-plasmid-map-sequence-id-267179

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  20. pHOXB6.1.0-gDNA

    Plasmid
    #132458
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    HOXB6 (HOXB6 Human)
    Use
    CRISPR
    Available Since
    Dec. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    132458-plasmid-map-sequence-id-266992

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Showing: 901 - 920 of 165214 results