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pAAV-ITR-EcYtR(NGS-6)-mCherry

Plasmid

#217363

Purpose

Expresses E. coli tyrosine tRNA variant "NGS-6" and a wild-type mCherry reporter; can be packaged into AAV

Depositor

Abhishek Chatterjee

Article

Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

Insert

E. coli tyrosine tRNA mutant, "NGS-6"

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-ITR-PytR(PyOtR)-mCherry

Plasmid

#204870

Purpose

Expresses pyrrolysyl tRNA variant "PyOtR" and a wild-type mCherry reporter; can be packaged into AAV

Depositor

Abhishek Chatterjee

Article

Jewel et al Angew Chem Int Ed Engl. 2024 Jan 26:e202

Insert

M. mazei pyrrolysyl tRNA mutant "PyOtR"

Use

AAV

Tags

Expression

Mammalian

Mutation

U25C; A3G, A4C, A5G, C6G, G63C, T65G, T66C

Promoter

U6

Available since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAW-BiP-Nb127D01-mCherry-KDEL

Plasmid

#171575

Purpose

Expresses mCherry-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-mCherry) in fly cell ER membrane

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

BiP-Nb127D01-mCherry-KDEL (CXCR2 Human)

Use

Tags

BiP signal peptide and mCherry-KDEL

Expression

Insect

Mutation

Promoter

fly actin5C promoter

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAW-BiP-NbVHH05-mCherry-KDEL

Plasmid

#171574

Purpose

Expresses mCherry-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-mCherry) in fly cell ER membrane

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

BiP-NbVHH05-mCherry-KDEL (UBE2J1 Human)

Use

Tags

BiP signal peptide and mCherry-KDEL

Expression

Insect

Mutation

Promoter

fly actin5C promoter

Available since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-miniDi-P2A-mCherry

Plasmid

#204358

Purpose

AAV vector for miniDi expression under the control of human synapsin promoter

Depositor

Masao Doi

Article

Miyake et al Cell Rep Methods. 2024 Oct 21;4(10):1008

Insert

miniDi-P2A-mCherry

Use

AAV

Tags

mCherry

Expression

Mutation

The third intracellular loop (ICL3) of hM4Di was …

Promoter

hSyn

Available since

Oct. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-miniDq-P2A-mCherry

Plasmid

#204357

Purpose

AAV vector for miniDq expression under the control of human synapsin promoter

Depositor

Masao Doi

Article

Miyake et al Cell Rep Methods. 2024 Oct 21;4(10):1008

Insert

miniDq-P2A-mCherry

Use

AAV

Tags

mCherry

Expression

Mutation

The third intracellular loop (ICL3) of hM3Dq was …

Promoter

hSyn

Available since

Oct. 22, 2024

Availability

Academic Institutions and Nonprofits only

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GfaABC1D BARK D110A p2A mCherry

Plasmid

#117692

Purpose

GfaABC1D-iBARK(D110A)-p2A-mCherry: Negative control viral expression vector for astrocyte Gaq silencing

Depositor

Baljit Khakh

Article

Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

Insert

BARKrgs D110A peptide

Use

AAV

Tags

HA / FLAG

Expression

Mutation

D110A

Promoter

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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AttB_ACE2_IRES-mCherry-H2A-P2A-PuroR

Plasmid

#171594

Purpose

Strong human ACE2 abundance from Bxb1 landing pad

Depositor

Kenneth Matreyek

Article

Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

Inserts

ACE2 (ACE2 Human)
mCherry
H2A
Pac

Use

Synthetic Biology; Promoterless bxb1 dna recombin…

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pCXLE-hOCT3/4-shp53-F + mCherry-2A-puro

Plasmid

#74947

Purpose

Dosage control and tracing of reprogramming episomes

Depositor

Ann Zovein

Article

Schmitt et al Stem Cell Res Ther. 2017 Jun 5;8(1):132.

Insert

Oct3/4 (POU5F1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-rev-ChR2(H134R)-mCherry

Plasmid

#18916

Purpose

Cre-dependent viral expression of ChR2(H134R)-mCherry for neuronal excitation

Depositor

Scott Sternson

Article

Atasoy et al J Neurosci. 2008 Jul 9. 28(28):7025-30.

Insert

Channelrhodopsin 2-mCherry

Use

AAV and Cre/Lox; Adeno-associated virus

Tags

mCherry

Expression

Mutation

H134R

Promoter

CAG

Available since

Jan. 29, 2009

Availability

Academic Institutions and Nonprofits only

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LSM061 (PB)SFFV-LIDARsensor(mcherry-BFP) (hygR)

Plasmid

#233427

Purpose

PiggyBac donor plasmid with SFFV driven LIDAR reporter RNA (mCherry as marker, TagBFP as output)

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

LIDARsensor(mcherry-BFP)

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pHR-PGK-ABI-dCas9-P2A-mcherry

Plasmid

#121513

Purpose

expression of AB1-fused-dcas9 plasmids

Depositor

Stanley Qi

Article

Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

Insert

dCas9

Use

Lentiviral

Tags

AB1 and P2A-mcherry

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pX552-EF1a-DIO-mcherry(with gRNA scaffold)

Plasmid

#199581

Purpose

Expresses mCherry in a Cre-dependent manner

Depositor

Bryan Copits

Article

Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

Insert

mCherry

Use

AAV, CRISPR, and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

EF1a intron A and U6

Available since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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p403 – TDH3-MTS-mCherry-GFP1-10-His

Plasmid

#177730

Purpose

mCherry and the 1-10th β-strands of GFP (GFP1-10) fused to a mitochondria signal sequence for mitochondrial targeting in yeast plasmid with HIS marker

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

mCherry-GFP1-10

Use

Tags

Mitochondrial Targeting Sequence

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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EK0313 SFFV-mCherry-SG(s70587)SG-Cre (FLP-IN)

Plasmid

#191149

Purpose

Expression of Sensor 1 (for EK0208) in mammalian cells; mCherry marker, Cre output derived from a gift from prof. Liqun Luo

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:s70587:Cre

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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EK0344 SFFV-mCherry-SG(Bdnf.s1)SG-Cre (FLP-IN)

Plasmid

#191158

Purpose

Expression of a sensor for murine Bdnf 3' UTR in mammalian cells; mCherry marker, Cre output; 90 bp long

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:Bdnf.s1:Cre

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgMap3K12(1)-U6-sgMap3K12(2)-hSyn1-mCherry

Plasmid

#208835

Purpose

Knockout of Map3K12 (DLK) using tandem sgRNAs, expresses mCherry under the hSyn1 promoter

Depositor

Ben Emery

Article

Duncan et al Nat Commun. 2024 Oct 23;15(1):9148. doi:

Insert

mCherry

Use

AAV and CRISPR

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

hSyn1

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgMap3K13(1)-U6-sgMap3K13(2)-hSyn1-mCherry

Plasmid

#208836

Purpose

Knockout of Map3K13 (LZK) using tandem sgRNAs, expresses mCherry under the hSyn1 promoter

Depositor

Ben Emery

Article

Duncan et al Nat Commun. 2024 Oct 23;15(1):9148. doi:

Insert

mCherry

Use

AAV and CRISPR

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

hSyn1

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgMap3K13(1)-U6-sgMap3K12(1)-hSyn1-mCherry

Plasmid

#208837

Purpose

Knockout of Map3K12 (DLK) and Map3K13 (LZK) using tandem sgRNAs, expresses mCherry under the hSyn1 promoter

Depositor

Ben Emery

Article

Duncan et al Nat Commun. 2024 Oct 23;15(1):9148. doi:

Insert

mCherry

Use

AAV and CRISPR

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

hSyn1

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA4TO F8.2(S54L+T127A)-T2A-mCherry

Plasmid

#225586

Purpose

Mammalian expression vector for Doxycycline inducible expression of F8.2(S54L+T127A) anti-tau nanobody with a self-cleaving T2A-mCherry fluorescent expression reporter

Depositor

William McEwan

Article

Benn et al Science. 2024 Aug 30;385(6712):1009-1016

Insert

F8.2(S54L+T127A)-T2A-mCherry

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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