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pMAX-DEST

Plasmid

#37631

Depositor

Valeri Vasioukhin

Article

Klezovitch et al Proc Natl Acad Sci U S A. 2008 Feb 12. 1

Type

Empty backbone

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2012

Availability

Academic Institutions and Nonprofits only
pHLsec hSOD1

Plasmid

#232481

Purpose

vector for transient transfection expressing human SOD1gene including flag-tag (DYKDDDDK) on the 3' end

Depositor

Lukas Trantirek

Article

Rynes et al Commun Biol. 2025 Feb 7;8(1):194. doi: 1

Insert

Homo sapiens superoxide dismutase 1 (SOD1 Human)

Tags

DYKDDDDK (FLAG-tag)

Expression

Mammalian

Promoter

CAG

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Omicron.BA.5-RBD

Plasmid

#213478

Purpose

Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.BA.5 variant

Depositor

Priyamvada Acharya

Article

Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

Insert

pCAGGS/Omicron.BA.5 RBD (S )

Tags

6 His Tag

Expression

Mammalian

Mutation

G339D,R346T,S371F,S373P,S375F,T376A,D405N,R408S,K…

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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PDLIM1

Plasmid

#38809

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

PDLIM1 (PDLIM1 Human)

Tags

His Tev

Expression

Bacterial

Mutation

Truncated (see comments)

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
PcDNA-DEST53-CypB-Nucl N-term GFP

Plasmid

#36123

Depositor

Patrick Van Oostveldt

Article

Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

Insert

cyclophilin B (PPIB Human, contains CypB AA 33-216)

Tags

GFP

Expression

Mammalian

Mutation

A212S

Promoter

CMV

Available Since

Nov. 1, 2012

Availability

Academic Institutions and Nonprofits only
PcDNA-DEST47-CypB-Del3 C-term GFP

Plasmid

#36136

Depositor

Patrick Van Oostveldt

Article

Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

Insert

cyclophilin B (PPIB Human)

Tags

GFP

Expression

Mammalian

Mutation

contains amino acids 39-216 of cyclophilin B; G63…

Promoter

CMV

Available Since

Nov. 1, 2012

Availability

Academic Institutions and Nonprofits only
PcDNA-DEST47-CypB-Del1 C-term GFP

Plasmid

#36132

Depositor

Patrick Van Oostveldt

Article

Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

Insert

cyclophilin B (PPIB Human)

Tags

GFP

Expression

Mammalian

Mutation

contains amino acids 37-216 of cyclophilin B

Promoter

CMV

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
PcDNA-DEST53-CypB-Golgi N-term GFP

Plasmid

#36127

Depositor

Patrick Van Oostveldt

Article

Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

Insert

cyclophilin B (PPIB Human)

Tags

GFP

Expression

Mammalian

Mutation

contains amino acids 1-211 of cyclophilin B

Promoter

CMV

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
PcDNA-DEST53-CypB-Del4 N-term GFP

Plasmid

#36137

Depositor

Patrick Van Oostveldt

Article

Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

Insert

cyclophilin B (PPIB Human)

Tags

GFP

Expression

Mammalian

Mutation

contains amino acids 42-216 of cyclophilin B

Promoter

CMV

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
pAAV_BiSSTe4_dTomato_nlsdTomato

Plasmid

#213944

Purpose

AAV construct expressing dTomato driven by SST interneuron-targeting enhancer. Alias: AAV_WDS0004_dTomato_nlsdTomato

Depositor

Gordon Fishell

Article

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Has Service

AAV PHP.eB and AAV1

Insert

dTomato_P2A_nls.dTomato

Use

AAV

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pRho-Cre

Plasmid

#13779

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

Rhodopsin promoter-Cre

Use

Cre/Lox

Tags

Myc

Expression

Mammalian

Available Since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

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tTA-IRES-Neo

Plasmid

#16541

Depositor

Bert Vogelstein

Article

Yu et al Proc Natl Acad Sci U S A. 1999 Dec 7. 96

Insert

tTA

Expression

Mammalian

Available Since

April 16, 2015

Availability

Academic Institutions and Nonprofits only
pCF525-mTagBFP2_EF1a-Hygro-P2A-mTagBFP2_lenti

Plasmid

#138315

Purpose

Lenti expression of mTgBFP2

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

mTAGBFP2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

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pET26b-Granzyme A

Plasmid

#8823

Depositor

Judy Lieberman

Article

Beresford et al Proc Natl Acad Sci U S A 1997 Aug 19;94(

Insert

Human Granzyme A (GZMA Human)

Tags

(His)6 and Enterokinase site

Expression

Bacterial

Mutation

it mature enzyme.

Available Since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only

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SSH-pcDNA3.1-SSH-SARS-CoV-2-S-WT

Plasmid

#218460

Purpose

Mammalian expression plasmid for SARS-CoV-2 spike (1273 residues)

Depositor

Syed Saif Hasan

Article

Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

Insert

SARS-CoV-2 spike protein (1273 residues)-wild type (S )

Expression

Mammalian

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pTA-attP

Plasmid

#18939

Depositor

Michele Calos

Article

Groth et al Proc Natl Acad Sci U S A. 2000 May 23. 9

Insert

phiC31 attP

Expression

Bacterial

Available Since

Oct. 17, 2008

Availability

Academic Institutions and Nonprofits only

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pJFRC153-20XUAS-IVS-B2::PEST

Plasmid

#32134

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

B2::PEST

Expression

Insect

Available Since

Sept. 23, 2011

Availability

Academic Institutions and Nonprofits only

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Shim_ProRS_3278.3.7_SHI28108_TP_GFP_pK7fWG2_IVD_FP611

Plasmid

#228379

Purpose

Expresses N-term transit peptide from Sapria himalayana Proline aminoacyl-tRNA synthetase evm.model.fragScaff scaffold 3278.3.7 (SHI28108RA); determination of S. himalayana ProRS target localization

Depositor

Daniel Sloan

Article

DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

N-terminal transit peptide from S. himalayana ProRS evm.model.fragScaff scaffold 3278.3.7 (SHI28108RA)

Tags

Green Florescent Protien

Expression

Plant

Available Since

Feb. 26, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pBPhsFlp1

Plasmid

#32148

Purpose

Expresses FLP recombinase in Drosophila

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

hsFlp1

Expression

Insect

Mutation

Aspartic Acid at Amino Acid 5

Promoter

Hsp70Bb promoter

Available Since

Sept. 23, 2011

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-Alpha

Plasmid

#177961

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (Alpha: del69,70, del144, D614G, N501Y, A570D, P681H, T716I, S982A, D1118H)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (Alpha: del69, 70, del144, D614G, N501Y, A570D, P681H, T716I, S982A, D1118H) (S )

Expression

Mammalian

Mutation

del69, 70, del144, D614G, N501Y, A570D, P681H, T7…

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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