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pHH0103_NCF1-2/2

Plasmid

#91492

Purpose

Protein expression and purification of human SH3 domain construct NCF1-2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

NCF1-2/2 (NCF1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_NCF1-1/2

Plasmid

#91305

Purpose

Protein expression and purification of human SH3 domain construct NCF1-1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

NCF1-1/2 (NCF1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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HASP_HUMAN_D0

Plasmid

#79730

Purpose

This plasmid encodes the kinase domain of HASP. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

HASP

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
KCC2D_HUMAN_D0

Plasmid

#79734

Purpose

This plasmid encodes the kinase domain of KCC2D. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

KCC2D

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
AAPK2_HUMAN_D0

Plasmid

#79738

Purpose

This plasmid encodes the kinase domain of AAPK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

AAPK2

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
KCC1G_HUMAN_D0

Plasmid

#79745

Purpose

This plasmid encodes the kinase domain of KCC1G. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

KCC1G

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
KCC2A_HUMAN_D0

Plasmid

#79746

Purpose

This plasmid encodes the kinase domain of KCC2A. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

KCC2A

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
KC1G2_HUMAN_D0

Plasmid

#79716

Purpose

This plasmid encodes the kinase domain of KC1G2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

KC1G2

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
MK03_HUMAN_D0

Plasmid

#79688

Purpose

This plasmid encodes the kinase domain of MK03. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

MK03

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
PAK4_HUMAN_D0

Plasmid

#79698

Purpose

This plasmid encodes the kinase domain of PAK4. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

PAK4 (PAK4 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
PAK6_HUMAN_D0

Plasmid

#79703

Purpose

This plasmid encodes the kinase domain of PAK6. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

PAK6 (PAK6 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
Sema3g.a(S)-Fc-His

Plasmid

#72152

Purpose

Expresses the Sema3G, isoform a protein (truncated at cleavage site P1; ie, short), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3g.a (Sema3g Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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Sema3g.z(S)-Fc-His

Plasmid

#72153

Purpose

Expresses the Sema3G, isoform z protein (truncated at cleavage site P1; ie, short), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3g.z (Sema3g Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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Unc5a.a-AP-His

Plasmid

#72050

Purpose

Expresses the extracellular region of the Unc5A, isoform a protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Unc5a.a (Unc5a Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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Sema4f.a-Fc-His

Plasmid

#72158

Purpose

Expresses the extracellular region of the Sema4F, isoform a protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema4f.a (Sema4f Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Sema6d.5-Fc-His

Plasmid

#72171

Purpose

Expresses the extracellular region of the Sema6D, isoform 5 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema6d.5 (Sema6d Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Sema3d(S)-Fc-His

Plasmid

#72144

Purpose

Expresses the Sema3D protein (truncated at cleavage site P1; ie, short), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3d (Sema3d Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Unc5c.z-AP-His

Plasmid

#72056

Purpose

Expresses the extracellular region of the Unc5C, isoform z protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Unc5c.z (Unc5c Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2016

Availability

Academic Institutions and Nonprofits only

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Unc5d.y-AP-His

Plasmid

#72058

Purpose

Expresses the extracellular region of the Unc5D, isoform y protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Unc5d.y (Unc5d Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Unc5d.z-AP-His

Plasmid

#72059

Purpose

Expresses the extracellular region of the Unc5D, isoform z protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Unc5d.z (Unc5d Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge