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pJC2297 - pHR-TRE3G-hSpCas9-NLS-FLAG-2A-Thy1.1

Plasmid

#250251

Purpose

Expression of Cas9 with Thy1.1 cell surface marker under a doxycycline responsive promoter. Requires additional expression of transactivator.

Depositor

Jacob Corn

Article

Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:

Insert

hSpCas9

Use

CRISPR and Lentiviral

Tags

NLS-FLAG-2A-Thy1.1

Promoter

TRE3G

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pCBS-4804

Plasmid

#249320

Purpose

Expresses Non-targeting sgRNA and contains the Repair Template to replace the KanR* on the genome of E. coli MG1655

Depositor

Chase Beisel

Article

Gupta et al Nat Biotechnol. 2025 Sep 4. doi: 10.1038

Insert

nontargeting sgRNA

Expression

Bacterial

Mutation

N/A

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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pCBS-5740

Plasmid

#249327

Purpose

Expresses sgRNA targeting the fcy gene in S. cerevisiae

Depositor

Chase Beisel

Article

Gupta et al Nat Biotechnol. 2025 Sep 4. doi: 10.1038

Insert

sgRNA targeting fcy

Expression

Yeast

Mutation

N/A

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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pSBtet-FAP-TGN38-GFP

Plasmid

#242992

Purpose

Inducible FAP-TGN38-GFP expression in mammalian cells

Depositor

Itay Budin

Article

Moore et al Nat Chem Biol (2025). https://doi.org/10

Insert

dH6.2-TGN38-GFP (Tgoln2 Rat)

Use

Sleeping beauty

Tags

FAP and GFP

Expression

Mammalian

Promoter

Tight TRE

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pSBtet-GFP-TGN38-FAP

Plasmid

#242993

Purpose

Inducible GFP-TGN38-FAP expression in mammalian cells

Depositor

Itay Budin

Article

Moore et al Nat Chem Biol (2025). https://doi.org/10

Insert

GFP-TGN38-dH6.2 (Tgoln2 Rat)

Use

Sleeping beauty

Tags

FAP and GFP

Expression

Mammalian

Promoter

Tight TRE

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pSBtet-mCer3-TGN38-FAP

Plasmid

#242994

Purpose

Inducible mCer3-TGN38-FAP expression in mammalian cells

Depositor

Itay Budin

Article

Moore et al Nat Chem Biol (2025). https://doi.org/10

Insert

mCer3-TGN38-dH6.2 (Tgoln2 Rat)

Use

Sleeping beauty

Tags

FAP and mCer3

Expression

Mammalian

Promoter

Tight TRE

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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LentiX_(loxPconDIAL-YB_TATA)-mCherry-HRasG12V-BGH_pKG3906

Plasmid

#246345

Purpose

loxPcon DIAL Reporter Lentivirus expressing mCherry-HRasG12V in the presence of ZFa

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

mCherry-GS-HRasG12V (HRAS Human)

Use

Lentiviral and Synthetic Biology

Mutation

G12V

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTCB-trasHCknob6xHis

Plasmid

#237364

Purpose

pTwist CMV BetaGlobin encoding trastuzumab heavy chain with knob Fc and 6xHis tag

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol. 2025 Dec 16. doi: 10.103

Insert

trastuzumab heavy chain

Use

Affinity Reagent/ Antibody

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pTCB-Sig9FcholeHA

Plasmid

#237367

Purpose

pTwist CMV BetaGlobin encoding Siglec-9-Fc with hole Fc and HA tag

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol. 2025 Dec 16. doi: 10.103

Insert

Siglec-9-Fc (SIGLEC9 Human)

Use

Affinity Reagent/ Antibody

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pTCB-Sig9R120AFcholeHA

Plasmid

#237369

Purpose

pTwist CMV BetaGlobin encoding Siglec-9-Fc R120A mutant with hole Fc and HA tag

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol. 2025 Dec 16. doi: 10.103

Insert

Siglec-9-Fc (R120A mutant) (SIGLEC9 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pTCB-Sig10FcholeHA

Plasmid

#237370

Purpose

pTwist CMV BetaGlobin encoding Siglec-10-Fc with hole Fc and HA tag

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol. 2025 Dec 16. doi: 10.103

Insert

Siglec-10-Fc (SIGLEC10 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pTCB-magroHCLALAPGknob6xHis

Plasmid

#237375

Purpose

pTwist CMV BetaGlobin encoding magrolimab heavy chain with LALA P329G knob Fc and 6xHis tag

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol. 2025 Dec 16. doi: 10.103

Insert

magrolimab heavy chain (silent Fc)

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pEf1a-Dn29-dCas9-P2A-GFP

Plasmid

#247155

Purpose

Mammalian expression of a human codon optimized wildtype Dn29 recombinase fused to dCas9 and gRNA expression cassette for targeting attH1 with H1-g3

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

Dn29-dCas9-P2A-GFP

Tags

SV40 NLS

Expression

Mammalian

Promoter

Ef1a

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pSG5-CAGEc-ABL1 (Npu)

Plasmid

#241420

Purpose

Proximity-triggered Protein Trans-Splicing to generate the splice product BCR-ABL, Figure 3, Extended Figure 6-7 in Nature Chemical Biology 20.10 (2024): 1353-1360.

Depositor

Thomas Muir

Article

Lee et al Nat Chem Biol. 2024 Oct;20(10):1353-1360

Insert

CAGEc-ABL1 (Npu) (ABL1 Human, Synthetic)

Tags

HA

Expression

Mammalian

Promoter

SV40

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV5-NUP98n-CAGEn-NES (Npu)

Plasmid

#241417

Purpose

Proximity-triggered Protein Trans-Splicing to generate the splice product MLL-AF9, Extended Figure 4 in Nature Chemical Biology 20.10 (2024): 1353-1360.

Depositor

Thomas Muir

Article

Lee et al Nat Chem Biol. 2024 Oct;20(10):1353-1360

Insert

NUP98n-CAGEn-NES (Npu) (NUP98 Human, Synthetic)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pSG5-DNAJB1n_CAGEn (Nrdj1)

Plasmid

#241422

Purpose

Proximity-triggered Protein Trans-Splicing to generate the splice product DNAJ-PKAc, Figure 4, Extended Figure 8-9 in Nature Chemical Biology 20.10 (2024): 1353-1360.

Depositor

Thomas Muir

Article

Lee et al Nat Chem Biol. 2024 Oct;20(10):1353-1360

Insert

DNAJB1n_CAGEn (Nrdj1) (DNAJB1 Human, Synthetic)

Tags

Myc

Expression

Mammalian

Promoter

SV40

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV5-BCRn-CAGEn (Npu)

Plasmid

#241419

Purpose

Proximity-triggered Protein Trans-Splicing to generate the splice product BCR-ABL, Figure 3, Extended Figure 6-7 in Nature Chemical Biology 20.10 (2024): 1353-1360.

Depositor

Thomas Muir

Article

Lee et al Nat Chem Biol. 2024 Oct;20(10):1353-1360

Insert

BCRn-CAGEn (Npu) (BCR Human, Synthetic)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV5-NUP98n-CAGEn-NES (Nrdj1)

Plasmid

#241418

Purpose

Proximity-triggered Protein Trans-Splicing to generate the splice product MLL-AF9, Figure 2 & Extended Figure 4 in Nature Chemical Biology 20.10 (2024): 1353-1360.

Depositor

Thomas Muir

Article

Lee et al Nat Chem Biol. 2024 Oct;20(10):1353-1360

Insert

NUP98n-CAGEn-NES (Nrdj1) (NUP98 Human, Synthetic)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pET-hIRF5-V5(4D)-LPETG

Plasmid

#237225

Purpose

Expresses LPETG-tagged, constitutively active human IRF5 variant in bacteria

Depositor

Elliot Chaikof

Article

Eweje et al Nat Biotechnol. 2025 May 15. doi: 10.103

Insert

Interferon regulatory factor 5 (IRF5 Human)

Tags

6xHis

Expression

Bacterial

Mutation

S451D, S453D, S456D, S462D

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pINDUCER20-hGfi1

Plasmid

#121200

Purpose

inducible expression of hGfi1 in colorectal cancer cells

Depositor

Noah Shroyer

Article

Chen et al Mol Cancer Res. 2019 Mar;17(3):697-708.

Insert

Growth factor independent 1 transcriptional (GFI1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

P2L

Available Since

July 25, 2025

Availability

Academic Institutions and Nonprofits only

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