We narrowed to 14,471 results for: flag

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pSplit-OC-U5-40K

Plasmid

#51741

Purpose

Express U5-40K-LucC fusion protein in mammalian cells

Depositor

Hiroyoshi Ariga

Article

Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

Insert

U5-40K (SNRNP40 Human)

Tags

FLAG and Luciferase (394-550aa)

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2014

Availability

Academic Institutions and Nonprofits only
MSCV JMJD3 mutant

Plasmid

#21214

Depositor

Paul Khavari

Article

Sen et al Genes Dev. 2008 Jul 15. 22(14):1865-70.

Insert

JMJD3 (KDM6B Human)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Mutation

H1390A

Available Since

June 23, 2009

Availability

Academic Institutions and Nonprofits only

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pCDNA3-STAT3-S727D

Plasmid

#73364

Purpose

Expression of STAT3-S727D

Depositor

Jie Chen

Article

Kim et al J Biol Chem. 2009 Dec 18;284(51):35425-3

Insert

Signal Transducer And Activator Of Transcription 3 (Acute-Phase Response Factor)

Tags

FLAG tagged

Expression

Mammalian

Mutation

E16K S727D

Promoter

CMV

Available Since

May 23, 2016

Availability

Academic Institutions and Nonprofits only

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pLV hUbC-dCas9 KRAB-T2A-GFP

Plasmid

#67620

Purpose

Co-expressed human optimized S. pyogenese dCas9 fused to KRAB repressor domain and GFP

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Insert

humanized dead Cas9 KRAB T2A GFP

Use

CRISPR and Lentiviral

Tags

Flag

Mutation

D10A and H840A

Available Since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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pLV hUbC-Cas9-T2A-GFP

Plasmid

#53190

Purpose

3rd generation transfer vector. Co-expresses human optimized S. pyogenes Cas9 and GFP

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Insert

humanized Cas9 T2A GFP

Use

CRISPR and Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

hUbC

Available Since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

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pLV hUbC-dCas9-T2A-GFP

Plasmid

#53191

Purpose

Co-expresses human optimized S. pyogenes dCas9 and GFP

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Insert

humanized dead Cas9 T2A GFP

Use

CRISPR and Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

D10A and H840A

Promoter

hUbC

Available Since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

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CBla_2.0_EmrE_TMD4_3P

Plasmid

#207848

Purpose

BLaTM-System; Antiparallel positive control EmrE_TMD4_3P in CBLa 2.0 vector

Depositor

Dieter Langosch

Article

Julius et al J Mol Biol. 2017 Apr 18. pii: S0022-2836

Insert

C-BLa 2.0 fusion protein

Tags

Flag (DYKDHDG)

Expression

Bacterial

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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CBla_2.0_EmrE_TMD4_3P_G90V_G97V

Plasmid

#207849

Purpose

BLaTM-System; Antiparallel negativecontrol EmrE_TMD4_3P_G90V_G97V in CBLa 2.0 vector

Depositor

Dieter Langosch

Article

Julius et al J Mol Biol. 2017 Apr 18. pii: S0022-2836

Insert

C-BLa 2.0 fusion protein

Tags

Flag (DYKDHDG)

Expression

Bacterial

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLV hUbC-dCas9 VP64-T2A-GFP

Plasmid

#53192

Purpose

Co-expresses human optimized S. pyogenes dCas9-VP64 and GFP

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Insert

humanized dead Cas9 VP64 T2A GFP

Use

CRISPR and Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

D10A and H840A

Promoter

hUbC

Available Since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

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pUC57-TPNOX

Plasmid

#87853

Purpose

This plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.

Depositor

Vamsi Mootha

Article

Cracan et al Nat Chem Biol. 2017 Aug 7. doi: 10.1038/

Insert

TPNOX

Tags

C-terminal FLAG tag with a linker

Expression

Bacterial

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pRG646_lexO-HO_LexA-TF_LEU2MX

Plasmid

#154815

Purpose

Inducible expression of HO endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Article

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

HO endonuclease (HO Budding Yeast)
LexA-ER-B112

Tags

FLAG

Expression

Yeast

Promoter

ACT1 and lexO_4-CYC1_core

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-NPM WT

Plasmid

#17578

Depositor

Xin Wang

Article

Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

Insert

NPM (NPM1 Human)

Tags

EGFP and Flag

Expression

Mammalian

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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nuclear cpVenus control

Plasmid

#186790

Purpose

Identical to nuclear NAD+ biosensor, sans bipartite NAD+-binding domain modeled from bacterial DNA ligase

Depositor

Michael Cohen

Article

Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

Insert

nuclear cpVenus control

Use

Lentiviral

Expression

Mammalian

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pScI_dCas9-CDA_J23119-sgRNA

Plasmid

#108550

Purpose

Bacterial Target-AID vector with sgRNA

Depositor

Akihiko Kondo

Article

Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

Inserts

dCas9-PmCDA1
Streptococcus pyogenes sgRNA

Tags

Flag

Expression

Bacterial

Mutation

D10A and H840A for SpCas9

Promoter

J23119 and lambda OR

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV_psychLight2

Plasmid

#163910

Purpose

Expresses psychLight2 in mammalian cells

Depositor

Lin Tian

Article

Dong et al Cell. 2021 Apr 8. pii: S0092-8674(21)003

Insert

psychLight2

Tags

Flag tag

Expression

Mammalian

Promoter

CAG

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3-STAT3-Y705F

Plasmid

#74434

Purpose

Expression of STAT3-Y705F

Depositor

Jie Chen

Article

Kim et al J Biol Chem. 2009 Dec 18;284(51):35425-3

Insert

Signal Transducer And Activator Of Transcription 3 (Acute-Phase Response Factor)

Tags

Flag

Expression

Mammalian

Mutation

Mutations E16K, Y705F

Promoter

CMV

Available Since

May 23, 2016

Availability

Academic Institutions and Nonprofits only

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pTriEx-NTOM20-mVenus-Zdk2

Plasmid

#81011

Purpose

Expression of Zdk2 anchored to mitochondria

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

Zdk2

Tags

6x His tag, FLAG, NTOM20, and mVenus

Expression

Bacterial, Insect, and Mamm…

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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AAV.CAG.GLPLight1

Plasmid

#187467

Purpose

Expresses GLPLight1 in mammalian cells

Depositor

Tommaso Patriarchi

Article

Duffet et al Elife. 2023 Jun 2;12:RP86628. doi: 10.75

Insert

GLPLight1

Use

AAV

Tags

Flag tag

Promoter

CAG

Available Since

Feb. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLDPuro-hsnSR100N

Plasmid

#35172

Depositor

Benjamin Blencowe

Article

Raj et al Mol Cell. 2011 Sep 2;43(5):843-50.

Insert

SRRM4 (SRRM4 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only

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pBiex-1 GFPNanobody_SNAP

Plasmid

#82711

Purpose

Expresses a GFP binding nanobody linked to a SNAP tag in bacteria and Sf9 cells.

Depositor

Sivaraj Sivaramakrishnan

Article

Sommese et al Protein Sci. 2016 Aug 18. doi: 10.1002/p

Insert

GFP Nanobody

Tags

6xHis Purification tag, FLAG purification tag, an…

Expression

Bacterial and Insect

Promoter

T7 Promoter

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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