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LentiCRISPR-B_2xsgRNAs-RPPH1 (pAVA3585)

Plasmid

#239262

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting RPPH1

Depositor

Andrei Alexandrov

Publication

Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

Insert

U6-driven sgRNA1 and 7SK-driven sgRNA2 targeting RPPH1

Use

CRISPR and Lentiviral

Available since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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POC1553

Plasmid

#238470

Purpose

pMOBC360_VL: Vector Left (VL) of the set (Chloramphenicol R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach

Depositor

Christopher A. O'Callaghan

Publication

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET-21_MBP-VidN

Plasmid

#241013

Purpose

VidN construct that can be bacterially expressed, used to demonstrate intein splicing reaction.

Depositor

Cole DeForest

Publication

Gharios et al Bioconjug Chem. 2024 Jun 19;35(6):750-75

Insert

MBP-VidN

Tags

MBP-tag

Expression

Bacterial

Promoter

T7

Available since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVAb847

Plasmid

#217502

Purpose

pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb84 backbone, pRO1600/ColE1 ori

Depositor

Nico Claassens

Publication

van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

Insert

superfolder green fluorescent protein (sfGFP)

Use

Synthetic Biology

Mutation

S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

Available since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVAb867

Plasmid

#217503

Purpose

pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb86 backbone, p15A ori

Depositor

Nico Claassens

Publication

van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

Insert

superfolder green fluorescent protein (sfGFP)

Use

Synthetic Biology

Mutation

S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

Available since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVAb897

Plasmid

#217505

Purpose

pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb89 backbone, pBR322/ROP ori

Depositor

Nico Claassens

Publication

van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

Insert

superfolder green fluorescent protein (sfGFP)

Use

Synthetic Biology

Mutation

S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

Available since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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POC1554

Plasmid

#238471

Purpose

pMOBC360_VR: Vector Right (VR) of the set (Chloramphenicol R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach

Depositor

Christopher A. O'Callaghan

Publication

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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H1x

Plasmid

#218111

Purpose

Encodes mus musculus histone 1 in Gateway entry vector

Depositor

Peter Hamilton

Publication

Kim et al Transl Psychiatry. 2024 Jun 5;14(1):239.

Insert

Histone H1x (H1f10 Mouse)

Use

Gateway cloning entry vector

Expression

Bacterial

Available since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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mAID(KR)–2×GFP NB(WT)

Plasmid

#232304

Purpose

KR GFP adaptor for C. elegans

Depositor

Masato Kanemaki

Publication

Islam et al J Cell Sci. 2025 Jun 9:jcs.263961. doi:

Inserts

KR GFP adaptor
OsTIR1(F74G)

Expression

Worm

Available since

June 16, 2025

Availability

Academic Institutions and Nonprofits only

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mAID(WT)–GFP NB(WT)

Plasmid

#232297

Purpose

WT GFP nanbody adaptor

Depositor

Masato Kanemaki

Publication

Islam et al J Cell Sci. 2025 Jun 9:jcs.263961. doi:

Inserts

WT GFP adaptor
OsTIR1(F74G)

Expression

Mammalian

Available since

June 16, 2025

Availability

Academic Institutions and Nonprofits only

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mAID(KR)–GFP NB(KR)

Plasmid

#232298

Purpose

KR GFP nanobody adaptor

Depositor

Masato Kanemaki

Publication

Islam et al J Cell Sci. 2025 Jun 9:jcs.263961. doi:

Type

Empty backbone

Expression

Mammalian

Available since

June 16, 2025

Availability

Academic Institutions and Nonprofits only

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POC1525

Plasmid

#226496

Purpose

pMOBC360_VM: Vector Middle (VM) of the set (Chloramphenicol R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach

Depositor

Christopher A. O'Callaghan

Publication

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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POC1520

Plasmid

#221581

Purpose

pMOBK360_VR: Vector Right (VR) of the set (Kanamycin R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach

Depositor

Christopher A. O'Callaghan

Publication

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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POC1355

Plasmid

#221572

Purpose

Assembly vector designed to be methylated by the M.Osp807II BsaI-associated switch methylase and for the assembly of inserts 1, 2, 3 and 4 from POC1343, POC1344, POC1345 and POC1346; respectively

Depositor

Christopher A. O'Callaghan

Publication

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1430

Plasmid

#221577

Purpose

Assembly vector designed to be methylated by the M.Osp807II BsaI-associated switch methylase and for the assembly of inserts 1, 2, 3 and 4 from POC1426, POC1427, POC1428 and POC1429; respectively

Depositor

Christopher A. O'Callaghan

Publication

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1518

Plasmid

#221579

Purpose

pMOBK360_VL: Vector Left (VL) of the set (Kanamycin R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach

Depositor

Christopher A. O'Callaghan

Publication

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
POC1519

Plasmid

#221580

Purpose

pMOBK360_VM: Vector Middle (VM) of the set (Kanamycin R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach

Depositor

Christopher A. O'Callaghan

Publication

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pOET3-6xHis-hSMSr-ΔSAMD

Plasmid

#236225

Purpose

Express N-terminal His-tagged human SMSr (SAMD8)-sterile alpha motif domain deletion mutant protein in insect cells

Depositor

Chiaki Murakami

Publication

Murakami et al J Biol Chem. 2021 Jan-Jun;296:100454. do

Insert

SAMD8 (SAMD8 Human)

Tags

Hexa histidine tag (6xHis-tag) and enterokinase s…

Expression

Insect

Mutation

sterile alpha motif domain at N-terminal (1-231 b…

Available since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pAP-U2-1

Plasmid

#236472

Purpose

Plasmid for integration at the native URA3 locus with 3xmCherry and 3xGFP under control of the strong bidirectional SpH2A/B promoter.

Depositor

Daniel Lew

Publication

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

spH2Ap-3xmCherry spH2Bp-3xGFP

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available since

April 14, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAP-U2-3

Plasmid

#236474

Purpose

Plasmid for integration at the native URA3 locus with 3xmCherry under control of the ScACT1 promoter and 3xGFP under control of the ApACT1 promoter.

Depositor

Daniel Lew

Publication

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

scACT1p-3xmCherry apACT1p-3xGFP

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available since

April 14, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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