We narrowed to 9,613 results for: Coli

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pRSFDuet-1_mNeongreen-Erythropoietin

Plasmid

#182422

Purpose

Bacterial expression of mNeongreen-Erythropoietin fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

mNeongreen-eythropoietin (EPO Human)

Tags

mNeongreen

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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meffBlue chromoprotein

Plasmid

#117845

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses meffBlue chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, meffBlue

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available Since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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YFP-S100A10

Plasmid

#107200

Purpose

Expresses YFP-human S100A10 fusion protein for live cell imaging

Depositor

Volker Gerke, Ursula Rescher

Article

Zobiack et al J Cell Sci. 2002 Jan 1;115(Pt 1):91-8.

Insert

S100A10 (S100A10 Human)

Tags

Yellow Fluorescent Protein

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2018

Availability

Academic Institutions and Nonprofits only

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pAGM22082_cRed

Plasmid

#117225

Purpose

pET-28b backbone plasmid, Level 2 Golden Gate expression plasmid.

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Sci Rep. 2019 Jul 29;9(1):10932. doi: 10

Insert

Canthaxanthin Operon

Tags

34 amino acid N-terminal linker, Hexahistidine Ta…

Expression

Bacterial

Mutation

removed Type IIS restriction sites within the bac…

Promoter

T7 RNA Polymerase

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-mNeongreen-DARPinOFF7

Plasmid

#182415

Purpose

Bacterial expression of Vesicle Nucleating peptide-mNeongreen-DARPinOFF7 fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-mNeongreen-DARPinOFF7

Tags

Vesicle Nucleating peptide (VNp) & mNeongreen

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pET22b-T5-sfGFP*- MmH-MH4R

Plasmid

#196409

Purpose

Express sfGFP with TAG and enzymes for biosynthesizing DOPA

Depositor

Han Xiao

Article

Chen et al J Mol Biol. 2021 Dec 20:167412. doi: 10.

Inserts

sfGFP with TAG at 151 position
Tyrosine Hydroxylase (Th Mouse)
dihydropterine reductase from human (QDPR Human)
pterin-4a-carbinolamine dehydratase from human

Expression

Bacterial

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pHS0923 pET45b(+) His14-MBP-TEV-AmaTnpB

Plasmid

#176587

Purpose

Bacterial expression of His14-MBP-TEV-AmaTnpB

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

Inserts

MBP tag
TnpB

Tags

His14 tag

Expression

Bacterial

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pMM627

Plasmid

#112532

Purpose

pZE2-PLhrtO-lux-hrtR-RBS2-chuA - Composite plasmid of pMM534 and pMM549, ColE1 origin, KanR

Depositor

Timothy Lu

Article

Mimee et al Science. 2018 May 25;360(6391):915-918.

Inserts

HrtR
ChuA
luxCDABE

Expression

Bacterial

Promoter

J23107, PL(HrtO), and ProD

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-itis pBE-nt

Plasmid

#195343

Purpose

Base editor plasmid expressing ABE8e-NG-Cas9n under Theophylline Riboswitch and constituitively expressed nonsense non-targeting gRNA

Depositor

Alexis Komor

Article

Vasquez et al CRISPR J. 2023 Apr 20. doi: 10.1089/cris

Inserts

ecTadA(8e)-SpCas9-NG
gRNA gtgcacgacgccgtatgcga

Use

CRISPR

Mutation

SpCas9n-NG: D10A, L1111R, D1135V, G1218R, E1219F,…

Promoter

Lac and ProC

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pEM-Cas9HF1-indRecA56

Plasmid

#102294

Purpose

Modified from pEM-Cas9HF1 (Addgene ID: 89961) to co-express inducible recA56 to block recA-mediated double-strand break repair.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Inserts

Cas9HF1
recA56

Use

CRISPR

Expression

Bacterial

Mutation

N497A/R661A/Q695A/Q926A

Promoter

pTet

Available Since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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