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AiP1011-pAAV-mscRE4-minBGpromoter-tTA2-WPRE-hGHpA

Plasmid

#163480

Purpose

Direct-expressing tTA2 AAV Virus. Alias: AiP1011 - pAAV-AiE2004m-minBG-tTA2-WPRE-HGHpA

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

tTA2

Use

AAV

Promoter

Beta Globin minimal promoter

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-SomaGCaMP6f2

Plasmid

#158756

Purpose

Cell body-targeted GCaMP6f, under synapsin promoter: GCaMP6f followed by a linker, and the EE-RR coiled-coil motif (GCaMP6f-27-EE-RR). As bright as conventional GCaMP6f.

Depositor

Edward Boyden

Article

Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

Insert

SomaGCaMP6f2

Use

AAV

Expression

Mammalian

Promoter

synapsin promoter

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pGP-CAG-Ace2N-4AA-mNeon A122D-WPRE-bGH-polyA

Plasmid

#172911

Purpose

Mammalian expression of voltage sensor

Depositor

GENIE Project

Article

Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

Insert

Ace2N-4AA-mNeon A122D

Tags

soma localization targeting sequence

Expression

Mammalian

Mutation

A122D

Promoter

CAG

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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TDP-43 F147/149L GFP

Plasmid

#237545

Purpose

Expression of GFP-tag fused to mutant TDP-43 F147/19L

Depositor

Tatyana Shelkovnikova

Article

Hodgson et al Nat Cell Biol. 2026 Mar 18. doi: 10.1038

Insert

GFP-tagged TDP-43 F147/149L (TARDBP Human)

Tags

EGFP

Expression

Mammalian

Mutation

TDP-43 F147L and F149L

Promoter

CMV

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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AiP981-pAAV-mscRE4-minBGpromoter-EGFP-WPRE-hGHpA

Plasmid

#163484

Purpose

Direct-expressing EGFP AAV Virus. Alias: AiP981 - pAAV-AiE2004m-minBG-EGFP-WPRE-HGHpA

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

EGFP

Use

AAV

Promoter

Beta Globin minimal promoter

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pDNA5-FRT/TO-FLAG-TONSL

Plasmid

#221011

Purpose

mammalian expression vector of Flag tagged TONSL siRNA resistant

Depositor

Daniel Durocher

Article

O'Donnell et al Mol Cell. 2010 Nov 24;40(4):619-31. doi:

Insert

TONSL (TONSL Human)

Tags

Flag

Expression

Mammalian

Mutation

silent mutations in TONSL: 5’-gaATtAgaTCtaTCGatga…

Promoter

CMV

Available Since

June 24, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pPHAGE_DLD-S456A-C-TAP

Plasmid

#83479

Purpose

Mammalian expression of the proteolysis-deficient mutant DLD-S456A.

Depositor

Mohan Babu

Article

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

DLD (DLD Human)

Use

Lentiviral

Tags

TAP

Expression

Mammalian

Mutation

S456A

Promoter

CMV

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pPHAGE_DLD-H450A-C-TAP

Plasmid

#83478

Purpose

Mammalian expression of the dehydrogenase-deficient mutant DLD-H450A.

Depositor

Mohan Babu

Article

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

DLD (DLD Human)

Use

Lentiviral

Tags

TAP

Expression

Mammalian

Mutation

H450A

Promoter

CMV

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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AiP1002-pAAV-mscRE16-minBGpromoter-EGFP-WPRE-hGHpA

Plasmid

#163486

Purpose

Direct-expressing EGFP AAV Virus. Alias: AiP1002 - pAAV-AiE2016m-minBG-EGFP-WPRE-HGHpA

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

EGFP

Use

AAV

Promoter

Beta Globin minimal promoter

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-FRT/TO-FLAG-SHLD2

Plasmid

#114117

Purpose

Expresses N-terminally tagged FLAG-SHLD2 in mammalian cells

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

SHLD2 (SHLD2 Human)

Use

Genomic integration, tetracycline inducible

Tags

FLAG

Expression

Mammalian

Promoter

CMV/TetO2

Available Since

Aug. 28, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-Syn-SomaGCaMP6f1

Plasmid

#158755

Purpose

Cell body-targeted GCaMP6f under synapsin promoter: GCaMP6f followed by a linker, the tail region of AnkyrinG, and an ER export motif (GCaMP6f-27-AnkTail-motif-ER2). Not as bright as SomaGCaMP6f2.

Depositor

Edward Boyden

Article

Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

Insert

SomaGCaMP6f1

Use

AAV

Expression

Mammalian

Promoter

synapsin promoter

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pGV13 (CK2alpha K68M, CK2beta)

Plasmid

#27094

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Inserts

CK2beta (CSNK2B Human)
CK2alpha K68M (CSNK2A1 Human)

Use

Tetracycline-regulated expression

Tags

HA on CK2alpha and Myc on CK2beta

Expression

Mammalian

Mutation

K68M on CK2alpha

Promoter

bidirectional tet-responsive promoter and bidirec…

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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AiP1078-pAAV-mscRE16-minBGpromoter-oNigri-WPRE-hGHpA

Plasmid

#163490

Purpose

Direct-expressing oNigri AAV Virus. Alias: AiP1078 - pAAV-AiE2016m-minBG-oNigri-WPRE-HGHpA

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

oNigri

Use

AAV

Promoter

Beta Globin minimal promoter

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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AiP995-pAAV-mscRE10-minBGpromoter-EGFP-WPRE-hGHpA

Plasmid

#163485

Purpose

Direct-expressing EGFP AAV Virus. Alias: AiP995 - pAAV-AiE2010m-minBG-EGFP-WPRE-HGHpA

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

EGFP

Use

AAV

Promoter

Beta Globin minimal promoter

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDNA5-FRT/TO-eGFP-TONSL

Plasmid

#221012

Purpose

mammalian expression vector of eGFP tagged TONSL siRNA resistant

Depositor

Daniel Durocher

Article

O'Donnell et al Mol Cell. 2010 Nov 24;40(4):619-31. doi:

Insert

TONSL (TONSL Human)

Tags

eGFP

Expression

Mammalian

Mutation

silent mutations in TONSL: 5’-gaATtAgaTCtaTCGatga…

Promoter

CMV

Available Since

June 24, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
AiP1048-pAAV-mscRE10-minBGpromoter-tTA2-WPRE-hGHpA

Plasmid

#163481

Purpose

Direct-expressing tTA2 AAV Virus. Alias: AiP1048 - pAAV-AiE2010m-minBG-tTA2-WPRE-HGHpA

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

tTA2

Use

AAV

Promoter

Beta Globin minimal promoter

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGP-CAG-Ace2N-4AA-mNeon-ST A122D-WPRE-bGH-polyA

Plasmid

#172912

Purpose

Mammalian expression of voltage sensor; soma localization targeting sequence

Depositor

GENIE Project

Article

Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

Insert

Ace2N-4AA-mNeon-ST A122D

Tags

soma localization targeting sequence

Expression

Mammalian

Mutation

A122D

Promoter

CAG

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP1050-pAAV-mscRE16-minBGpromoter-tTA2-WPRE-hGHpA

Plasmid

#163483

Purpose

Direct-expressing tTA2 AAV Virus. Alias: AiP1050 - pAAV-AiE2016m-minBG-tTA2-WPRE-HGHpA

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

tTA2

Use

AAV

Promoter

Beta Globin minimal promoter

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGV7 (CK2alpha' K69M, CK2beta)

Plasmid

#27095

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Inserts

CK2beta (CSNK2B Human)
CK2alpha' K69M (CSNK2A2 Human)

Use

Tetracycline-regulated expression

Tags

HA tag on CK2alpha' and Myc on CK2beta

Expression

Mammalian

Mutation

K69M on CK2alpha'

Promoter

bidirectional tet-responsive promoter

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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AiP11496 - pAAV-hDlxI56i-minBG-H2B-nsSYFP2-WPRE3-BGHpA (Alias: CN1496)

Plasmid

#164449

Purpose

Direct-expressing H2BSYFP2 AAV Virus

Depositor

Allen Institute for Brain Science, Boaz Levi

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

H2B-SYFP2

Use

AAV

Promoter

minBetaGlobin

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge