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pFREDusk-AmpR-MCS

Plasmid

#229737

Purpose

For far-red light-induced bacterial expression of gene of interest (AmpR)

Depositor

Heikki Takala

Article

Bohm et al ACS Synth Biol. 2025 Jul 11. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pRDB_268

Plasmid

#231112

Purpose

SpG-Cas9

Depositor

John Doench, David Root

Article

Kaplan et al Nat Genet. 2025 Nov;57(11):2920-2929. do

Insert

Cas9-SpG

Use

CRISPR and Lentiviral

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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AA566

Plasmid

#231115

Purpose

Fragmid fragment: (guide cassette) ABE activity-based selection cloning

Depositor

John Doench, David Root

Article

Kaplan et al Nat Genet. 2025 Nov;57(11):2920-2929. do

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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AA568

Plasmid

#231117

Purpose

Fragmid fragment: (guide cassette) CBE activity-based selection cloning

Depositor

John Doench, David Root

Article

Kaplan et al Nat Genet. 2025 Nov;57(11):2920-2929. do

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pNHA8

Plasmid

#223650

Purpose

BccP protein purification

Depositor

François-Xavier Campbell-Valois

Article

Haidar-Ahmad et al mSphere. 2024 Nov 21;9(11):e0055324. doi

Insert

BccP

Tags

MBP

Expression

Bacterial

Promoter

LacI

Available Since

Oct. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNHA11

Plasmid

#223643

Purpose

Expression of ipgC-turboID fusion

Depositor

François-Xavier Campbell-Valois

Article

Haidar-Ahmad et al mSphere. 2024 Nov 21;9(11):e0055324. doi

Inserts

ipgC (ipgC )
turboID

Tags

Myc

Expression

Bacterial

Promoter

rpsM

Available Since

Oct. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNHA12

Plasmid

#223644

Purpose

Expression of turboID-ipgC fusion

Depositor

François-Xavier Campbell-Valois

Article

Haidar-Ahmad et al mSphere. 2024 Nov 21;9(11):e0055324. doi

Inserts

ipgC (ipgC )
turboID

Tags

Myc

Expression

Bacterial

Promoter

rpsM

Available Since

Oct. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNHA13

Plasmid

#223645

Purpose

Expression of mxiE-turboID fusion

Depositor

François-Xavier Campbell-Valois

Article

Haidar-Ahmad et al mSphere. 2024 Nov 21;9(11):e0055324. doi

Inserts

mxiE (mxiE )
turboID

Tags

Myc

Expression

Bacterial

Promoter

rpsM

Available Since

Oct. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNHA14

Plasmid

#223646

Purpose

Expression of turboID-mxiE fusion

Depositor

François-Xavier Campbell-Valois

Article

Haidar-Ahmad et al mSphere. 2024 Nov 21;9(11):e0055324. doi

Inserts

mxiE (mxiE )
turboID

Tags

Myc

Expression

Bacterial

Promoter

rpsM

Available Since

Oct. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pAMC070n1a12

Plasmid

#209746

Purpose

ColA BBa_B0014 tetAp SD8 ScTrpRS rrnB1 proB SD8 Lum1PylRS BBaB10014RNAItrpA+ J23100 SD8 MmPylRS T3Te-T7Te DHFR

Depositor

Ahmed Badran

Article

Costello et al Nat Biotechnol. 2024 Sep 11. doi: 10.103

Insert

tRNA operon - ScTrpRS - Lum1PylRS - MmPylRS

Expression

Bacterial

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pNoT7

Plasmid

#218168

Purpose

pET31b plasmid with short insert lacking T7 and ribosome binding sites as control

Depositor

Emily Weinert

Article

Chauhan et al ACS Cent Sci. 2022 Nov 23;8(11):1518-152

Insert

Short sequence, mutated RBS and T7 sites

Expression

Bacterial

Promoter

NA - mutated T7

Available Since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-BBS18

Plasmid

#218718

Purpose

Expresses N-terminally EGFP-tagged BBS18 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

Insert

BBS18

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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Efg1-ΔDBD

Plasmid

#216396

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1

Depositor

Nicolas Fawzi

Article

Wang et al Biophys J. 2024 Jun 4;123(11):1481-1493.

Insert

Efg1-ΔDBD

Tags

6xHis-tags

Expression

Bacterial

Mutation

deleted amino acids 182-356

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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pHIT346

Plasmid

#204505

Purpose

5S rDNA used as a probe for chromosome FISH in nematode

Depositor

Yuji Kohara

Article

Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:

Insert

5S rDNA (C. elegans)

Use

Other

Promoter

T7

Available Since

Aug. 15, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-Bmp7

Plasmid

#201909

Purpose

in vitro transcription of RNA encoding FLAG-tagged Bmp7a

Depositor

Mary Mullins

Article

Little et al Nat Cell Biol. 2009 May;11(5):637-43. do

Insert

bmp7 (bmp7a Zebrafish)

Tags

FLAG tag

Promoter

SP6

Available Since

June 7, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NvH-CtNot1optim_1676-2193_U

Plasmid

#147729

Purpose

Bacterial Expression of CtNot1optim_1676-2193

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

CtNot1optim_1676-2193

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-RLuc-6xbsMS2-8xlet7_AC

Plasmid

#148433

Purpose

Mammalian Expression of 6xbsMS2-8xlet7

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

6xbsMS2-8xlet7

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-TISU-RLuc-6xbsMS2_AC

Plasmid

#148434

Purpose

Mammalian Expression of 6xbsMS2

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

6xbsMS2

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-TISU-RLuc-6xbsMS2-8xlet7_AC

Plasmid

#148435

Purpose

Mammalian Expression of 6xbsMS2-8xlet7

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

6xbsMS2-8xlet7

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-TISU-RLuc-6xbsMS2-random_AC

Plasmid

#148437

Purpose

Mammalian Expression of 6xbsMS2-random

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

6xbsMS2-random

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-no5UTR-lambdaN-HA-GST-V5His6_AC

Plasmid

#148405

Purpose

Insect Expression vector

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Type

Empty backbone

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1C-no5UTR-Fluc-5BoxB_AC

Plasmid

#148408

Purpose

Insect Expression of 5BoxB

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

5BoxB

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pEPYC1CB0597

Plasmid

#196166

Purpose

MoClo Level 1 luciferase reporter: MinSyn_301 reporter [MinSyn_301::TMV::LucF::flag::ocsT]

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

MinSyn_301 reporter [MinSyn_301::TMV::LucF::flag::ocsT]

Use

Synthetic Biology

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pEPYC1CB0602

Plasmid

#196168

Purpose

MoClo Level 1 luciferase reporter: MinSyn_303 reporter [MinSyn_303::TMV::LucF::flag::ocsT]

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

MinSyn_303 reporter [MinSyn_303::TMV::LucF::flag::ocsT]

Use

Synthetic Biology

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pEPYC1CB0601

Plasmid

#196167

Purpose

MoClo Level 1 luciferase reporter: MinSyn_302 reporter [MinSyn_302::TMV::LucF::flag::ocsT]

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

MinSyn_302 reporter [MinSyn_302::TMV::LucF::flag::ocsT]

Use

Synthetic Biology

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPYC1CB0604

Plasmid

#196169

Purpose

MoClo Level 1 luciferase reporter: MinSyn_305 reporter [MinSyn_305::TMV::LucF::flag::ocsT]

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

MinSyn_305 reporter [MinSyn_305::TMV::LucF::flag::ocsT]

Use

Synthetic Biology

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPYC1CB0607

Plasmid

#196171

Purpose

MoClo Level 1 luciferase reporter: MinSyn_310 reporter [MinSyn_308::TMV::LucF::flag::ocsT]

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

MinSyn_310 reporter [MinSyn_308::TMV::LucF::flag::ocsT]

Use

Synthetic Biology

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPYC1CB0608

Plasmid

#196172

Purpose

MoClo Level 1 luciferase reporter: MinSyn_311 reporter [MinSyn_308::TMV::LucF::flag::ocsT]

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

MinSyn_311 reporter [MinSyn_308::TMV::LucF::flag::ocsT]

Use

Synthetic Biology

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPYC1CB0595

Plasmid

#196170

Purpose

MoClo Level 1 luciferase reporter: MinSyn_308 reporter [MinSyn_308::TMV::LucF::flag::ocsT]

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

MinSyn_308 reporter [MinSyn_308::TMV::LucF::flag::ocsT]

Use

Synthetic Biology

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPYCeGM0022

Plasmid

#196147

Purpose

Gateway entry vector with Arabidopsis ARF18 CDS and c-terminal HiBiT tag

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

AtARF18_HiBit

Use

Gateway entry vector

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge