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Halo-HSF2

Plasmid

#200192

Purpose

Expression of full length HSF2

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

Halo-HSF2 (Hsf2 Mouse)

Tags

Halo

Expression

Mammalian

Mutation

None

Promoter

EF_1alpha

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO-DrSmg6_F

Plasmid

#146279

Purpose

Bacterial Expression of DrSmg6

Depositor

Elisa Izaurralde

Article

Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

Insert

DrSmg6 (LOC100334020 Zebrafish)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-tubcDNA-PTC33_D

Plasmid

#146141

Purpose

Mammalian Expression of tubcDNA-PTC33

Depositor

Elisa Izaurralde

Article

Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

Insert

tubcDNA-PTC33

Expression

Mammalian

Mutation

5 silent and one non silent mutation E442K compar…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-tubcDNA-PTC62_D

Plasmid

#146142

Purpose

Mammalian Expression of tubcDNA-PTC62

Depositor

Elisa Izaurralde

Article

Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

Insert

tubcDNA-PTC62

Expression

Mammalian

Mutation

5 silent and one non silent mutation E442K compar…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pET22b-T5-chi28TAG31TAG

Plasmid

#188996

Purpose

Express chimadanin with TAG at 28 and 31 position

Depositor

Han Xiao

Article

Chen et al Nat Commun. 2022 Sep 16;13(1):5434. doi:

Insert

Chimadanin with TAG at 28 and 31position

Expression

Bacterial

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pSEM262 - pDESTR4-R3 mosTI hygroR target

Plasmid

#159840

Purpose

Single copy or array insertion by MosTI using split hygroR selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

pDESTR4-R3 mosTI hygroR target

Expression

Worm

Mutation

Not applicable

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pSEM264 - pDESTR4-R3 mosTI Pmlc-2_GFP target

Plasmid

#159842

Purpose

Single copy or array insertion by MosTI using split Pmlc-2 GFP selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

pDESTR4-R3 mosTI Pmlc-2_GFP target

Expression

Worm

Mutation

Not applicable

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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PX552-mScn8a-3xHA-Syn-smFP-flag

Plasmid

#182563

Purpose

For mouse Nav1.6 knockin with 3xHA at the C-terminal

Depositor

James Zhe Liu

Article

Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

Inserts

mouse Scn8a gRNA and 3xHA donor DNA
smFP-flag

Use

AAV and CRISPR

Promoter

EF1 and U6

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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PX552-mScn8a-HaloTag-V5-EF1-sfGFP

Plasmid

#182565

Purpose

For mouse Nav1.6 knockin with HaloTag-V5 at the C-terminal

Depositor

James Zhe Liu

Article

Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

Inserts

mouse Scn8a gRNA and HaloTag-V5 donor DNA
sfGFP

Use

AAV and CRISPR

Promoter

EF1 and U6

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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PX552-mScn8a-3xV5-EF1-smFP-flag

Plasmid

#182562

Purpose

For mouse Nav1.6 knockin with 3xV5 at the C-terminal

Depositor

James Zhe Liu

Article

Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

Inserts

mouse Scn8a gRNA and 3xV5 donor DNA
smFP-flag

Use

AAV and CRISPR

Promoter

EF1 and U6

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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PB-TAC-Cas9

Plasmid

#120352

Purpose

piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry). SpCas9 is constitutively expressed.

Depositor

Knut Woltjen

Article

Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

Type

Empty backbone

Use

CRISPR; Piggybac transposon

Expression

Mammalian

Promoter

tet-on and CBh

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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PB-TAC-KRAB-dCas9

Plasmid

#120355

Purpose

piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry). KRAB-dCas9 is constitutively expressed.

Depositor

Knut Woltjen

Article

Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

Type

Empty backbone

Use

CRISPR; Piggybac transposon

Expression

Mammalian

Promoter

tet-on and CBh

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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YIplac204-pOst1-ESCargo(Orange)

Plasmid

#140157

Purpose

Expresses a regulatable secretory cargo for yeast at the TRP1 locus

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

pOst1-APVNTT-E2-Orange-FKBP(LV)(C22V)

Expression

Yeast

Promoter

TPI1

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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YIplac128-pOst1-ESCargo(Orange)

Plasmid

#140158

Purpose

Expresses a regulatable secretory cargo for yeast at the LEU2 locus

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

pOst1-APVNTT-E2-Orange-FKBP(LV)(C22V)

Expression

Yeast

Promoter

TPI1

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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YIplac128-pOst1-ESCargo(Crimson)

Plasmid

#140156

Purpose

Expresses a regulatable secretory cargo for yeast at the LEU2 locus

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

pOst1-APVNTT-E2-Crimson-FKBP(LV)(C22V)

Expression

Yeast

Promoter

TPI1

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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sgPYM1

Plasmid

#105249

Purpose

human PYM1 sgRNA

Depositor

Geraldine Seydoux

Article

Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

Insert

sgRNA for PYM1

Expression

Mammalian

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only
MLSE-shTAF12.376

Plasmid

#105578

Purpose

retrovirally express TAF12 shRNA with GFP marker

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAF12 shRNA #376

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

MSCV-LTR

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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MLSE-shRPA3.457

Plasmid

#105584

Purpose

retrovirally express RPA3 shRNA with GFP marker

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

RPA3 shRNA #457

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

MSCV-LTR

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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MLSE-shTAF12.364

Plasmid

#105577

Purpose

retrovirally express TAF12 shRNA with GFP marker

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAF12 shRNA #364

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

MSCV-LTR

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pSG5-MAGE-TA360

Plasmid

#89141

Purpose

mammalian expression of full-length human MAGE-A11 amino acid residues 2-429 with TA360 phosphorylation site mutated

Depositor

Elizabeth Wilson

Article

Bai et al Mol Cell Biol. 2008 Mar;28(6):1947-63. d

Insert

MAGE-A11 (MAGEA11 Human)

Expression

Mammalian

Mutation

T360A

Promoter

SV40

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only