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PLK4 G7.1 gRNA

Plasmid

#90836

Purpose

3rd generation lentiviral gRNA plasmid targeting human PLK4

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

PLK4 (Guide Designation G7.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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MIS18BP1 F10.1 gRNA

Plasmid

#90769

Purpose

3rd generation lentiviral gRNA plasmid targeting human MIS18BP1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

MIS18BP1 (Guide Designation F10.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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MCM6 E1.2 gRNA

Plasmid

#90762

Purpose

3rd generation lentiviral gRNA plasmid targeting human MCM6

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

MCM6 (Guide Designation E1.2)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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MASTL F4.1 gRNA

Plasmid

#90753

Purpose

3rd generation lentiviral gRNA plasmid targeting human MASTL

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

MASTL (Guide Designation F4.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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DYNLT3 B3.5 gRNA

Plasmid

#90670

Purpose

3rd generation lentiviral gRNA plasmid targeting human DYNLT3

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

DYNLT3 (Guide Designation B3.5)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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DYNLRB1 H11.3 gRNA

Plasmid

#90664

Purpose

3rd generation lentiviral gRNA plasmid targeting human DYNLRB1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

DYNLRB1 (Guide Designation H11.3)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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gh144

Plasmid

#106803

Purpose

expression of gRNA targeting LARGE

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

LARGE

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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Myc-gamma3/gamma2SIL

Plasmid

#121433

Purpose

GABAA receptor expression (chimeric rat gamma 3 subunit whose large intracellular loop has been replaced with the large intracellular loop of the rat gamma 2 short subunit) Myc-tag near N-terminus

Depositor

Angel de Blas

Insert

GABRG3/GABRG2 SIL (Gabrg2, Gabrg3 Rat)

Use

Tags

cMyc epitope EQKLISEEDL inserted between fourth a…

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2019

Availability

Academic Institutions and Nonprofits only

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Myc-gamma1/gamma2SIL

Plasmid

#121432

Purpose

GABAA receptor expression (chimeric rat gamma 1 subunit whose large intracellular loop has been replaced with the large intracellular loop of the rat gamma 2 short subunit) Myc-tag near N-terminus

Depositor

Angel de Blas

Insert

GABRG1/GABRG2 SIL (Gabrg1, Gabrg2 Rat)

Use

Tags

cMyc epitope EQKLISEEDL inserted between fourth a…

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2019

Availability

Academic Institutions and Nonprofits only

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TGIF2 A11.3 gRNA

Plasmid

#90910

Purpose

3rd generation lentiviral gRNA plasmid targeting human TGIF2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

TGIF2 (Guide Designation A11.3)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-R1ACR_877-EYFP

Plasmid

#103883

Purpose

Anion channelrhodopsin from Rhodomonas sp. (CCMP768) for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

Insert

Anion channelrhodopsin R1ACR_877

Use

Tags

EYFP

Expression

Mammalian

Mutation

human codon optimized

Promoter

CMV

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-R1ACR_447-EYFP

Plasmid

#103960

Purpose

Anion channelrhodopsin from Rhodomonas sp. (CCMP768) for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

Insert

Anion channelrhodopsin R1ACR_447

Use

Tags

EYFP

Expression

Mammalian

Mutation

human codon optimized

Promoter

CMV

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-R2ACR_853-EYFP

Plasmid

#103963

Purpose

Anion channelrhodopsin from Rhodomonas sp. (CCAC1630) for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

Insert

Anion channelrhodopsin R2ACR_853

Use

Tags

EYFP

Expression

Mammalian

Mutation

human codon optimized

Promoter

CMV

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-C1ACR_023-EYFP

Plasmid

#103966

Purpose

Anion channelrhodopsin from unclassified cryptophyte (CCMP2293) for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

Insert

Anion channelrhodopsin C1ACR_023

Use

Tags

EYFP

Expression

Mammalian

Mutation

human codon optimized

Promoter

CMV

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-R1ACR_799-EYFP

Plasmid

#103962

Purpose

Anion channelrhodopsin from Rhodomonas sp. (CCMP768) for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

Insert

Anion channelrhodopsin R1ACR_799

Use

Tags

EYFP

Expression

Mammalian

Mutation

human codon optimized

Promoter

CMV

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-C1ACR_887-EYFP

Plasmid

#103965

Purpose

Anion channelrhodopsin from unclassified cryptophyte (CCMP2293) for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

Insert

Anion channelrhodopsin C1ACR_887

Use

Tags

EYFP

Expression

Mammalian

Mutation

human codon optimized

Promoter

CMV

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-PsuACR_433-EYFP

Plasmid

#103529

Purpose

Anion channelrhodopsin from Proteomonas sulcata (CCMP704) for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

Insert

Anion channelrhodopsin PsuACR_433

Use

Tags

EYFP

Expression

Mammalian

Mutation

human codon optimized

Promoter

CMV

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-G1ACR_203-EYFP

Plasmid

#103143

Purpose

Anion channelrhodopsin from Geminigera sp. (Caron Lab Isolate) for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

Insert

Anion channelrhodopsin G1ACR_203

Use

Tags

EYFP

Expression

Mammalian

Mutation

human codon optimized

Promoter

CMV

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-GcACR_145-EYFP

Plasmid

#103138

Purpose

Anion channelrhodopsin from Geminigera cryophila (CCMP2564) for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

Insert

Anion channelrhodopsin GcACR_145

Use

Tags

EYFP

Expression

Mammalian

Mutation

human codon optimized

Promoter

CMV

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-R1ACR_367-EYFP

Plasmid

#103773

Purpose

Anion channelrhodopsin from Rhodomonas sp. (CCMP768) for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

Insert

Anion channelrhodopsin R1ACR_367

Use

Tags

EYFP

Expression

Mammalian

Mutation

human codon optimized

Promoter

CMV

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge