We narrowed to 7,456 results for: Ski;

1. pJK044 (pET-PurQ-wt)

   Plasmid

   #73839

   Purpose

   Produces Bacillus subtilis formylglycinamidine ribonucleotide synthetase glutaminase subunit, (PurQ)

   Depositor

   T. J. Kappock, JoAnne Stubbe

   Article

   Hoskins et al Biochemistry. 2004 Aug 17;43(32):10314-2

   Insert

   formylglycinamidine ribonucleotide synthetase, glutaminase subunit
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCellFree_G03 RRN3

   Plasmid

   #67112

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   RRN3 (RRN3 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pCellFree_G03 TFAP4

   Plasmid

   #67073

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   TFAP4 (TFAP4 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   P338A

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

4. L284F Cus2p

   Plasmid

   #73059

   Purpose

   S. cerevisiae Cus2p gene with L284F Mutation

   Depositor

   Aaron Hoskins

   Article

   Rodgers et al RNA. 2015 Dec 2.

   Insert

   CUS2p (CUS2 Budding Yeast)
   

   Use

   Tags

   6x His tag fused to TEV cleavage site

   Expression

   Bacterial and Yeast

   Mutation

   Leucine 284 to Phenylalanine

   Promoter

   T7

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. D282N Cus2p

   Plasmid

   #73060

   Purpose

   S. cerevisiae Cus2p gene with D282N Mutation

   Depositor

   Aaron Hoskins

   Article

   Rodgers et al RNA. 2015 Dec 2.

   Insert

   CUS2p (CUS2 Budding Yeast)
   

   Use

   Tags

   6x His tag fused to TEV cleavage site

   Expression

   Bacterial and Yeast

   Mutation

   Asp 282 to Asn

   Promoter

   T7

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. WT Ecm2

   Plasmid

   #169922

   Purpose

   Ecm2 +/-300bp of up- and downstream seq. cloned into the NotI and SalI sites of pRS416

   Depositor

   Aaron Hoskins

   Article

   van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

   Insert

   Ecm2
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. VR124 pETv2 10His-pG-Tn5 (E54K, L372P)

   Plasmid

   #198468

   Purpose

   E. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in T7 Express lysY/Iq protein expression strain.

   Depositor

   Viviana Risca

   Article

   Soroczynski et al bioRxiv 2024.07.11.602973

   Insert

   10His-pG-Tn5 (E54K, L372P)
   

   Use

   Tags

   10His-pG

   Expression

   Bacterial

   Mutation

   E54K, L372P

   Promoter

   Consensus φ10 T7 Promoter

   Available Since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCXLE-dCas9VP192-T2A-EGFP-shP53

   Plasmid

   #69535

   Purpose

   Episomal plasmid encoding dCas9VP192 and p53 shRNA

   Depositor

   Timo Otonkoski

   Article

   Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

   Insert

   dCas9-dCas9VP192-GFP-shp53
   

   Use

   CRISPR

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   CAG

   Available Since

   Nov. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GFP-LacI

   Plasmid

   #183920

   Purpose

   Lac repressor labeled with GFP; can be used to label lacO sites

   Depositor

   Karsten Rippe

   Article

   Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

   Insert

   GFP-LacI (lacI Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   SV2

   Available Since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pscALPS- SIV-MAC251 vpx

    Plasmid

    #115810

    Purpose

    Encodes codon optimized 3xFLAG-VpxMAC251 and puromycin resistance protein

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    SIVMAC251 Vpx
    

    Use

    Lentiviral

    Tags

    3x-FLAG

    Expression

    Mutation

    Promoter

    SFFV

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CAP-AAV.FUS.3

    Plasmid

    #222333

    Purpose

    AAV.FUS.3 Rep-Cap plasmid for production of AAV.FUS.3, a capsid with tropism for CNS neurons upon focused ultrasound mediated delivery.

    Depositor

    Jerzy Szablowski, Mikhail Shapiro

    Article

    Li et al Nat Commun. 2024 Jun 10;15(1):4924. doi:

    Insert

    AAV9 modified with 7-amino acid insertion between AA position 588 and 589 of VP1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    p41

    Available Since

    Aug. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLEX-TCRb-FKBP-Blasticidin

    Plasmid

    #120723

    Purpose

    Expresses ER-targeted recruiter protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    T cell receptor β chain
    

    Use

    Lentiviral

    Tags

    FKBP and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCI-MEG3

    Plasmid

    #44727

    Depositor

    Anne Klibanski

    Article

    Zhou et al J Biol Chem. 2007 Aug 24;282(34):24731-4

    Insert

    Maternally Expressed Gene 3 (MEG3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. MBD2a pcDNA3.1

    Plasmid

    #78141

    Purpose

    Mammalian expression of MBD2a

    Depositor

    Heidi Schwarzenbach

    Article

    Wischnewski et al Mol Cancer Res. 2007 Jul;5(7):749-59.

    Insert

    MBD2a (MBD2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLENTI-CMV-GFP-TREX1 BLAST

    Plasmid

    #164228

    Purpose

    lentiviral plasmid for GFP-hTREX1

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    TREX1 (TREX1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCellFree_G03 CYP2D6

    Plasmid

    #67090

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    CYP2D6 (CYP2D6 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJEP207-AAV-CMV-MCS-pA

    Plasmid

    #78318

    Purpose

    AAV vector backbone containing a CMV promoter followed by a multiple cloning site(MCS) followed by a poly-Adenylation Signal (pA)

    Depositor

    Jonathan Ploski

    Article

    Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Cytomegalo Virus(CMV)

    Available Since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MBD2b pcDNA3.1

    Plasmid

    #78142

    Purpose

    Mammalian expression of MBD2b

    Depositor

    Heidi Schwarzenbach

    Article

    Wischnewski et al Mol Cancer Res. 2007 Jul;5(7):749-59.

    Insert

    MBD2b (MBD2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pCellFree_G03 CYP3A4

    Plasmid

    #67104

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    CYP3A4 (CYP3A4 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CX-nestinGFPas

    Plasmid

    #32401

    Depositor

    Patrick Salmon, Henryk Zulewski

    Article

    Eberhardt et al Biochem Biophys Res Commun. 2006 Jul 7;3

    Insert

    nestin promoter/enhancer GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge