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pLKO.1-puro-shFAM134B

Plasmid

#109013

Purpose

short-hairpin knockdown of targeted gene

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

shFAM134B (RETREG1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6 promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-CaMKIIa-hChR2(C128S)-EYFP-WPRE

Plasmid

#20294

Purpose

Lentiviral expression of ChR2 (C128S) Step Function Opsin (SFO) fused to EYFP, for bi-stable optogenetic excitation

Depositor

Karl Deisseroth

Article

Berndt et al Nat Neurosci. 2009 Feb . 12(2):229-34.

Insert

channelrhodopsin-2

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Mutation

changed Cysteine 128 to Serine

Promoter

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-GFP-FAM134B

Plasmid

#109026

Purpose

stable lentiviral expression of cDNA

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

FAM134B (RETREG1 Human)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

EIF-1a promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-hygro-mCherry-RAMP4

Plasmid

#118391

Purpose

stable lentiviral expression of cDNA

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

RAMP4 (SERP1 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

EIF-1a promoter

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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p005-RFP-strong

Plasmid

#108317

Purpose

Synthetic RFP based on Corynactis spp under strong synthetic constitutive promoter pFAB4005; kanamycin resistant.

Depositor

Jaroslaw Bryk

Article

Siddall et al bioRxiv 2021.06.20.449138

Insert

RFP under controlf of strong synthetic constitutive promoter pFAB4005

Use

Tags

Expression

Bacterial

Mutation

Promoter

pFAB4005

Available since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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rLAMP1-mCherry-EGFP-Tet-On

Plasmid

#109015

Purpose

Tet-On construct to induce expression of tandem eGFP-mCherry tagged mLAMP1 (lysosome marker)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

rLAMP1

Use

Lentiviral

Tags

mcherry-eGFP

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-CaMKIIa-ChETA-EYFP

Plasmid

#26967

Purpose

3rd gen lentiviral expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by CaMKIIa promoter for optogenetic activation

Depositor

Karl Deisseroth

Article

Gunaydin et al Nat Neurosci. 2010 Mar;13(3):387-92. doi

Insert

hChR2(E123T/H134R)-EYFP

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Mutation

E123T

Promoter

CaMKIIa

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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p006-Banana-Late

Plasmid

#112251

Purpose

ATF1 from Saccharomyces cerevisiae under osmY stationary phase promoter; kanamycin resistant.

Depositor

Jaroslaw Bryk

Article

Siddall et al bioRxiv 2021.06.20.449138

Insert

ATF1 under osmY promoter

Use

Tags

Expression

Bacterial

Mutation

Promoter

osmY

Available since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/VChR1-EYFP

Plasmid

#20955

Purpose

Mammalian expression of VChR1 fused to EYFP, for neuronal excitation

Depositor

Karl Deisseroth

Article

Zhang et al Nat Neurosci. 2008 Jun . 11(6):631-3.

Insert

volvox channelrhodopsin-1

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pGCG26

Plasmid

#82371

Purpose

YFP expressed from pJ23119 in the glpK locus with gentamicin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

YFP

Use

Tags

Expression

Mutation

Promoter

PJ23119

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pCas49

Plasmid

#82391

Purpose

rbcL-sfGFP expressed from PccmK2 promoter with Gmr in glpK

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

rbcL-GFP

Use

Tags

Expression

Mutation

Promoter

PccmK2

Available since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pALM179

Plasmid

#82023

Purpose

an updated version of cLac143 (pACSA_PcptOO_C38T,A39T_YFP_PMB2_LacIWF). The homologous region upstream of the inducible system was fixed to add a stop codon for A1837. No difference in expression

Depositor

Brian Pfleger

Article

Markley et al ACS Synth Biol. 2015 May 15;4(5):595-603

Insert

YFP

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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p005kanRFP

Plasmid

#58533

Purpose

RFP from coral Corynactis sp. under T5 promoter with lac operators; kanamycin resistant.

Depositor

Jaroslaw Bryk

Article

Siddall et al bioRxiv 2021.06.20.449138

Insert

Red fluorescent protein

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

T5 between two lac operators

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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Venus Cam KII alpha mutant T286D

Plasmid

#29429

Depositor

Steven Vogel

Article

Thaler et al Proc Natl Acad Sci U S A. 2009 Apr 14. 1

Insert

Venus CAM KII Alpha

Use

Tags

Venus

Expression

Mammalian

Mutation

T286D mutation in CamKIIa sequence

Promoter

Available since

July 5, 2011

Availability

Academic Institutions and Nonprofits only
pSB3C5-proD-B0032-E0051

Plasmid

#107241

Purpose

Expresses lacZa-GFP fusion from constitutive proD promoter

Depositor

Robert Sauer, Joseph Davis

Article

Davis et al Nucleic Acids Res. 2011 Feb;39(3):1131-4

Insert

ProA-RBS(B0032)-Gemini (E0051)

Use

Tags

Expression

Mutation

Promoter

proD

Available since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-CaMKIIa-hChR2(C128A)-EYFP-WPRE

Plasmid

#20293

Purpose

Lentiviral expression of ChR2 (C128A) Step Function Opsin (SFO) fused to EYFP, for bi-stable optogenetic excitation

Depositor

Karl Deisseroth

Article

Berndt et al Nat Neurosci. 2009 Feb . 12(2):229-34.

Insert

channelrhodopsin-2

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Mutation

changed Cysteine 128 to Alanine

Promoter

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
Venus Cam KII alpha mutant T286A

Plasmid

#29430

Depositor

Steven Vogel

Article

Thaler et al Proc Natl Acad Sci U S A. 2009 Apr 14. 1

Insert

Venus CAM KII Alpha

Use

Tags

Venus

Expression

Mammalian

Mutation

T286A mutation in CamKIIa sequence

Promoter

Available since

June 3, 2011

Availability

Academic Institutions and Nonprofits only
pLenti-CaMKIIa-hChR2(C128T)-EYFP-WPRE

Plasmid

#20295

Purpose

Lentiviral expression of ChR2 (C128T) Step Function Opsin (SFO) fused to EYFP, for bi-stable optogenetic excitation

Depositor

Karl Deisseroth

Article

Berndt et al Nat Neurosci. 2009 Feb . 12(2):229-34.

Insert

channelrhodopsin-2

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Mutation

changed Cysteine 128 to Threonine

Promoter

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pSB3C5-proA-B0032-E0051

Plasmid

#107244

Purpose

Expresses lacZa-GFP fusion from constitutive proA promoter

Depositor

Robert Sauer, Joseph Davis

Article

Davis et al Nucleic Acids Res. 2011 Feb;39(3):1131-4

Insert

ProA-RBS(B0032)-Gemini (E0051)

Use

Tags

Expression

Mutation

Promoter

proA

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pSB3C5-proC-B0032-E0051

Plasmid

#107242

Purpose

Expresses lacZa-GFP fusion from constitutive proC promoter

Depositor

Robert Sauer, Joseph Davis

Article

Davis et al Nucleic Acids Res. 2011 Feb;39(3):1131-4

Insert

ProC-RBS(B0032)-Gemini (E0051)

Use

Tags

Expression

Mutation

Promoter

proC

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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