We narrowed to 1,479 results for: pbluescript

1. pBT346_pLambda-attB-pCA-GFP-pA

   Plasmid

   #52551

   Purpose

   for producing PhiC31 attB-containing minicircle in vitro

   Depositor

   Liqun Luo

   Article

   Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

   Inserts

   Lambda integrase attL1
   Lambda integrase attR1
   PhiC31o attB (full length)
   GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PB-TAG-ERP2

   Plasmid

   #80479

   Purpose

   All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible GFP and constitutive rtTA and puromycin resistance)

   Depositor

   Knut Woltjen

   Article

   Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

   Type

   Empty backbone

   Use

   Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMK288 (mAID-Bsr)

   Plasmid

   #72826

   Purpose

   C-terminal tagging with mAID using CRISPR/Cas9

   Depositor

   Masato Kanemaki

   Article

   Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

   Insert

   mAID-Bsr
   

   Use

   CRISPR

   Tags

   mAID

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMK364 (CMV-OsTIR1-loxP-PURO-loxP)

   Plasmid

   #121184

   Purpose

   OsTIR1 introduction

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

   Insert

   CMV-OsTIR1-loxP-PURO-loxP
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   OsTIR1 codon optimized for human cells

   Promoter

   Available since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGWKS9

   Plasmid

   #139416

   Purpose

   Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mTurquoise2 marker.

   Depositor

   James Fraser

   Article

   Spencer et al Fungal Genet Biol. 2020 May;138:103365.

   Insert

   mTurquoise2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCRISPaint-T2A-Gal4-3xP3-RFP

   Plasmid

   #127556

   Purpose

   CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal4-SV40 and 3xP3-RFP visible eye marker.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   Gal4
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBlu/gRNA

   Plasmid

   #59188

   Purpose

   Serves as a shuttle vector for target oligo before insertion into Cas9 destination vector

   Depositor

   Robert Stupar

   Article

   Michno et al GM Crops Food. 2015;6(4):243-52. doi: 10

   Insert

   Guide RNA Cassette
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   U6 (Arabidopis)

   Available since

   Feb. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBT340_pattB-pCA-GFP-pA-FRT5-pPGK-Flpo-pA

   Plasmid

   #52549

   Purpose

   transgenic construct for expression of GFP and Flpo

   Depositor

   Liqun Luo

   Article

   Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

   Inserts

   PhiC31o attB (full length)
   GFP
   Flpo recombinase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pACT5FVMF(f)

   Plasmid

   #110549

   Purpose

   Control plasmid for titering FV by qPCR to determine the genome-containing particle number. This qPCR protocol is described in Hendrie et al., 2008

   Depositor

   David Russell

   Article

   Hendrie et al Mol Ther. 2008 Mar;16(3):534-40. doi: 10

   Insert

   EGFP, FV LTRs
   

   Use

   Qpcr control for titering foamy virus

   Tags

   Expression

   Mutation

   Promoter

   promoter: MLV LTR

   Available since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAID1.2-EF1a-NmScarlet-mAID

    Plasmid

    #140617

    Purpose

    Expression of OsTIR1 and mScarlet-mAID protein in mammalian cells under the control of EF1a promoter

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    OsTIR1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Mouse ZP3 promoter (JD#445)

    Plasmid

    #14634

    Depositor

    Jurrien Dean

    Article

    Rankin et al Development. 1998 Jul . 125(13):2415-24.

    Insert

    ZP3 promoter (Zp3 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 23, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMK281 (mCherry2-Hygro)

    Plasmid

    #72797

    Purpose

    C-terminal tagging with mCherry2 using CRIPSR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mCherry2-Hygro
    

    Use

    CRISPR

    Tags

    mCherry2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pT2HLacZpA1I.7 (#59)

    Plasmid

    #35965

    Depositor

    Thomas Sato

    Article

    Schlaeger et al Proc Natl Acad Sci U S A. 1997 Apr 1;94(

    Inserts

    Tie2 promoter (Tek Mouse)
    SDK LacZ
    SV40 pA
    Tie2 full enhancer
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAID1.2-EF1a-CmCherry-mAID

    Plasmid

    #140604

    Purpose

    Expression of OsTIR1 and mAID-mCherry protein in mammalian cells under the control of EF1a promoter

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    OsTIR1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBS hTSP-5

    Plasmid

    #13002

    Depositor

    Jack Lawler

    Article

    Newton et al Genomics. 1994 Dec . 24(3):435-9.

    Insert

    COMP (COMP Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 28, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pAID1.2-EF1a-NGFP-mAID

    Plasmid

    #140607

    Purpose

    Expression of OsTIR1 and GFP-mAID protein in mammalian cells under the control of EF1a promoter

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    OsTIR1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMK279 (mClover-Bsr)

    Plasmid

    #72795

    Purpose

    C-terminal tagging with mClover using CRIPSR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mClover-Bsr
    

    Use

    CRISPR

    Tags

    mClover

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDS24

    Plasmid

    #58952

    Purpose

    Encodes synthetic mitochondrial gene ARG8m in place of COX3 coding sequence. Flanked by mtDNA sequences allowing integration into rho+ mtDNA after transformation.

    Depositor

    Thomas Fox

    Article

    Steele et al Proc Natl Acad Sci U S A. 1996 May 28;93

    Insert

    Mitochondrially coded Arg8 protein
    

    Use

    Tags

    Expression

    Mutation

    Coding sequence synthetic in S. cerevisiae mitoch…

    Promoter

    n/a

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pSKB-

    Plasmid

    #62540

    Purpose

    Cloning vector to prepare modules flanked by appropriately designed BsaI sites for seamless assembly of multiple inserts by GoldenGate Cloning

    Depositor

    Eric Marois

    Article

    Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBS NL4-3 envFS eGFP PR-D25A

    Plasmid

    #101334

    Purpose

    pBS NL4-3 envFS eGFP with mutation that disrupts Protease catalytic activity

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    NL4-3 envFS
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Protease D25A

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge