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pOPTM p62 PB1 (1-122)

Plasmid

#210428

Purpose

Bacterial expression of MBP-PB1(1-122) with TEV cleavage site

Depositor

Carsten Sachse

Article

Tarafder et al Methods Mol Biol. 2019;1880:3-15. doi: 1

Insert

p62 PB1 (1-122) (NUP62 Human)

Tags

MBP

Expression

Bacterial

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pLXMA L1A

Plasmid

#153347

Purpose

Expression vector for plants with medium-copy number origin of replication

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Type

Empty backbone

Expression

Plant

Available Since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pETSUMOSyt1C2AB(96-421)

Plasmid

#209006

Purpose

Recombinant protein expression of truncated syt1 construct

Depositor

Edwin Chapman

Article

Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

Insert

syt1 (Syt1 Rat)

Tags

6xHis-SUMO

Expression

Bacterial

Mutation

aa 96-421 only

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pETmsGFPSyt1(80-142)

Plasmid

#209012

Purpose

Recombinant protein expression of truncated syt1 tagged with GFP

Depositor

Edwin Chapman

Article

Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

Insert

syt1 (Syt1 Rat)

Tags

6xHis-msfGFP

Expression

Bacterial

Mutation

aa 80-142 only

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pMx/ATP8A1-HA

Plasmid

#209225

Purpose

Mammalian expression of ATP8A1

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

Insert

ATP8A1 (ATP8A1 Human)

Use

Retroviral

Tags

HA

Available Since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pL0_CE-OriT

Plasmid

#202150

Purpose

Level 0 plasmid containing an origin of transfer sequence from pPtPBR11 required for making plasmids mobilizable for conjugation with CE overhangs used to build level 1 constructs.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Origin of transfer

Use

Synthetic Biology

Mutation

OriT sequence mutated to remove sapI sites domest…

Available Since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-ef1a-DIO-eGCaMP(plus)

Plasmid

#201149

Purpose

AAV virus production for Cre recombinase dependent expression of eGCaMP(plus)

Depositor

Andre Berndt

Article

Wait et al Res Sq. 2023 Aug 7:rs.3.rs-3146778. doi:

Insert

eGCaMP+

Use

AAV, Cre/Lox, and Mouse Targeting

Expression

Mammalian

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot3-F672AY676AF727A_S

Plasmid

#147505

Purpose

Mammalian Expression of HsNot3-F672AY676AF727A

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot3-F672AY676AF727A (CNOT3 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot2-W507E_S

Plasmid

#147510

Purpose

Mammalian Expression of HsNot2-W507E

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot2-W507E (CNOT2 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot2_2-429_S

Plasmid

#147485

Purpose

Mammalian Expression of HsNot2_2-429

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot2_2-429 (CNOT2 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot2_429-540_S

Plasmid

#147486

Purpose

Mammalian Expression of HsNot2_429-540

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot2_429-540 (CNOT2 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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SEC7-2Katushka-ter-Hygromycin

Plasmid

#184772

Purpose

Red marker for yeast late Golgi/early endosome. Integration of 2xKatushka tag at SEC7 C terminus. Uses antibiotic resistance marker hphMX6.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

SEC7 (SEC7 Budding Yeast)

Tags

2Katushka

Expression

Yeast

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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KBB382

Plasmid

#185085

Purpose

HA-tagged POM152 with glycosylation sites mutated

Depositor

Ken Belanger

Article

Belanger et al Genetics. 2005 Nov;171(3):935-47. doi: 1

Insert

POM152

Tags

HA

Expression

Yeast

Mutation

Pom152 N280A, N398A, N???A, N???A = Pom152Dglyc

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only
pBN086

Plasmid

#173448

Purpose

express E. coli E69 (O9a:K30) Wzc_K540M with Y715E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc_K540M with Y715E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

K540M, Y715E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN087

Plasmid

#173449

Purpose

express E. coli E69 (O9a:K30) Wzc_K540M with Y717E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc_K540M with Y717E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

K540M, Y717E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN088

Plasmid

#173450

Purpose

express E. coli E69 (O9a:K30) Wzc_K540M with Y718E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc_K540M with Y718E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

K540M, Y718E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN081

Plasmid

#173443

Purpose

express E. coli E69 (O9a:K30) Wzc_K540M with Y705E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc_K540M with Y705E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

K540M, Y705E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN082

Plasmid

#173444

Purpose

express E. coli E69 (O9a:K30) Wzc_K540M with Y706E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc_K540M with Y706E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

K540M, Y706E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN084

Plasmid

#173446

Purpose

express E. coli E69 (O9a:K30) Wzc_K540M with Y708E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc_K540M with Y708E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

K540M, Y708E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN085

Plasmid

#173447

Purpose

express E. coli E69 (O9a:K30) Wzc_K540M with Y713E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc_K540M with Y713E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

K540M, Y713E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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