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pDONR223-MOS

Plasmid

#23657

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MOS (MOS Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-SCYL3

Plasmid

#23680

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

SCYL3 (SCYL3 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pENTR-Site2

Plasmid

#24513

Depositor

Kazuo Kinoshita

Article

Uemura et al PLoS One. 2010 . 5(3):e9846.

Insert

Split RFP-GFP chimera

Use

Cre/Lox and Mouse Targeting

Expression

Mammalian

Available Since

May 5, 2010

Availability

Academic Institutions and Nonprofits only

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pET20b-delta133HtrA2 S142A

Plasmid

#16153

Depositor

L. Miguel Martins

Article

Plun-Favreau et al Nat Cell Biol. 2007 Nov;9(11):1243-52. d

Insert

HtrA2 (HTRA2 Human)

Tags

His

Expression

Bacterial

Mutation

S142A

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pET20b-delta133HtrA2 S400D

Plasmid

#16156

Depositor

L. Miguel Martins

Article

Plun-Favreau et al Nat Cell Biol. 2007 Nov;9(11):1243-52. d

Insert

HtrA2 (HTRA2 Human)

Tags

His

Expression

Bacterial

Mutation

S400D

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
TrmD_HaeIn/pET28b(+) N,C-tag

Plasmid

#12665

Depositor

SeWon Suh

Article

Kim et al Acta Crystallogr D Biol Crystallogr. 200

Insert

tRNA (guanine-N(1)-)-methyltransferase (trmD Haemophilus influenzae)

Tags

His

Expression

Bacterial

Available Since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

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Tad-CBE(TCA3)

Plasmid

#235064

Purpose

Expresses Tad-CBE(TCA3) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(TCA3)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pMK463 (BSD-P2A-mAB)

Plasmid

#214389

Purpose

mAID-BromoTag for N-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

BSD-P2A-mAID-BromoTag

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pSIQ-EGFP

Plasmid

#216239

Purpose

All-in One IQ transgene switch for EGFP expression

Depositor

Hyunju Ro

Article

Hong et al Mol Ther Methods Clin Dev. 2024 Feb 2;32

Insert

EGFP

Use

Synthetic Biology

Expression

Mammalian

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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pLX209-neo-HA-Ub-T2A-Tet3G

Plasmid

#202436

Purpose

Bicistronic expression of HA-tagged Ubiquitin (HA-Ubiquitin) and Tet-On 3G transactivator (Tet3G)

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

Ubiquitin

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1alpha

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pKrox24(2xD-E)DsRed

Plasmid

#200111

Purpose

Fluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

modified EGR1 promoter

Expression

Mammalian

Mutation

highly modified sequence

Promoter

2 copies of D-E element, no other promoter elemen…

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-rsChRmine-eYFP-WPRE

Plasmid

#183531

Purpose

Optogenetics

Depositor

Karl Deisseroth

Article

Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

Insert

rsChRmine-eYFP

Use

AAV

Mutation

I146M/G174S

Promoter

CaMKIIa

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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CMV::TVA/tRFP

Plasmid

#164486

Purpose

Dual CMV promoter plasmid to express TVA and TurboRFP

Depositor

Hollis Cline

Article

Faulkner et al eNeuro. 2021 Jun 7;8(4):ENEURO.0477-20.2

Inserts

TVA (CD320 Chicken)
TurboRFP

Expression

Mammalian

Promoter

sCMV and sCMV IE94

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR E2 HSPter-RPS5Ap-mito

Plasmid

#163146

Purpose

Plant mitoTALEN vector assembly, Entry clone 2 with attL1 L4; Multisite Gateway entry vector to terminate the E1 ORF and having RPS5A promoter with mitochondrial presequence for the E3 ORF

Depositor

Shin-ichi Arimura

Article

Arimura et al Plant J. 2020 Oct 24. doi: 10.1111/tpj.1

Insert

A terminator of Arabidopsis heat shock protein, RPS5A promoter, and mitochondrial presequence

Use

Gateway Cloning

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-Hygro-sgSIK3

Plasmid

#138699

Purpose

Expresses a human SIK3-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK3 human (SIK3 Human)

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pKK-FLAG-TEV

Plasmid

#105768

Purpose

Expression of your protein of interest in fusion with FLAG at the N-terminus. The tag is cleavable by TEV protease or enterokinase.

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

FLAG-TEV

Expression

Mammalian

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pKK-TEV-mCherry

Plasmid

#105796

Purpose

Expression of your protein of interest in fusion with red fluorescent protein at the C-terminus (cleavable by TEV), (PMID: 15558047). mCherry descends from the first truly monomeric red FP mRFP1.

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

TEV-mCherry

Expression

Mammalian

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pET MBP mClover LIC cloning vector (MBP-mClover)

Plasmid

#86930

Purpose

pET LIC cloning vector for Biotin-His6-MBP-N10-tev-yORF-mClover

Depositor

Chris Jeans

Type

Empty backbone

Tags

6xHis, AviTag (biotin), MBP, TEV cleavage site, a…

Expression

Bacterial

Promoter

T7

Available Since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv1-sgSHMT1-G3

Plasmid

#83903

Purpose

stable knockout

Depositor

David Sabatini

Article

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

Serine hydroxymethyltransferase 1 (SHMT1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGWB430

Plasmid

#68695

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

tdTomato

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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