We narrowed to 9,801 results for: pET

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pAG415Gal_Gcn4 WW+GFP

Plasmid

#233003

Purpose

Galactose iduced expression of Gcn4 WW+GFP in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 WW+

Tags

HA-GFP-HA and TEV cleavage site

Expression

Yeast

Mutation

E109N, S117D, T121W, N126W, T132W

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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ppAG415Gal_Gcn4 L113A+

Plasmid

#232972

Purpose

Galactose iduced expression of Gcn4 L113A in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 L113A

Tags

TEV cleavage site

Expression

Yeast

Mutation

L113A, E114Y, N126H, D127E, S136A

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 StoL

Plasmid

#232981

Purpose

Galactose iduced expression of Gcn4 StoL in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 StoL

Tags

TEV cleavage site

Expression

Yeast

Mutation

S104L, S117L, S136L, S144L

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 R3W

Plasmid

#232975

Purpose

Galactose iduced expression of Gcn4 R++ W+ in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 R++ W+

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

D103R, N126W, D133R, K143R

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAIP4_1BKR

Plasmid

#234168

Purpose

Heterologous protein expression of spectrin beta chain, non-erythrocytic 1 (Beta-II spectrin) in Escherichia coli

Depositor

Christopher Bahl

Insert

1BKR (SPTBN1 Human)

Tags

10x HisTag-Smt3

Expression

Bacterial

Promoter

T7

Available Since

March 6, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pINQ-ON10

Plasmid

#198689

Purpose

Periplasmic expression of anti-SARS-Cov-2 Nucleocapsid nanobody ON10 (HA-tagged) in BL21(DE3)

Depositor

Gualberto González Sapienza

Article

Segovia-de Los Santos et al Front Immunol. 2023 Jul 11;14:1220477. d

Insert

anti-SARS-CoV-2 Nucleocapsid nanobody ON10

Tags

6xHis tag, HA tag, OmpA signal peptide, and Ribos…

Expression

Bacterial

Promoter

T7 promoter

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pJB53

Plasmid

#226309

Purpose

E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

Depositor

David Savage

Article

Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

Insert

CsoS2 with V(T/S)G to AAA CsoS2 MR1-3

Expression

Bacterial

Mutation

VTG273-5AAA, VTG284-6AAA, VTG295-7AAA, VSG332-4AA…

Promoter

pTRC

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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H6Gb1-CREB(1-280) T2C, C90A, T198C

Plasmid

#220235

Purpose

bacterial expression of CREB1 domains

Depositor

Peter Wright

Article

Martinez-Yamout et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

CREB1 (Creb1 Rat)

Tags

His6-GB1-tev

Expression

Bacterial

Mutation

T2C, C90A, T198C

Promoter

T7

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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H6Gb1-CREB(1-280) C90A, T172C, T259C

Plasmid

#220234

Purpose

bacterial expression of CREB1 domains

Depositor

Peter Wright

Article

Martinez-Yamout et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

CREB1 (Creb1 Rat)

Tags

His6-GB1-tev

Expression

Bacterial

Mutation

C90A, T172C, T259C

Promoter

T7

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pTcI38-PRM-wasabi

Plasmid

#195554

Purpose

Temperature-dependent expression of mWasabi reporter with transcription activation by TcI38

Depositor

Mikhail Shapiro

Article

Xiong et al ACS Synth Biol. 2022 Jul 15;11(7):2518-2

Inserts

mWasabi
TcI38

Tags

6x His and AANDENYADAS degradation tag

Expression

Bacterial

Mutation

A67T, M1V, L65S, K68R, F115L, D126G, D188G

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-Creb5(T59/T61A)(HA)

Plasmid

#195025

Purpose

Gateway vector containing HA-tagged Creb5 with T59/T61A mutation

Depositor

Andrew Lassar, Cheng-Hai Zhang

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

HA-tagged Creb5 (full length, 508aa; with with T59/T61A mutation) (CREB5 Bovine)

Use

Gateway Cloning

Tags

3xHA

Mutation

T59/T61A

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-Creb5(T59/T61D)(HA)

Plasmid

#195026

Purpose

Gateway vector containing HA-tagged Creb5 with T59/T61D mutation

Depositor

Andrew Lassar, Cheng-Hai Zhang

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

HA-tagged Creb5 (full length, 508aa; with with T59/T61D mutation) (CREB5 Bovine)

Use

Gateway Cloning

Tags

3xHA

Mutation

T59/T61D

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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p11d-RPA70/14 (MSW#134)

Plasmid

#208076

Purpose

Expression of human Replication Protein A 70 and 14-kDa subunits in E. coli with 14 having a His-tag

Depositor

Marc Wold

Article

Henricksen et al J Biol Chem. 1994 Apr 15;269(15):11121-3

Insert

RPA1, RPA3 (RPA1, RPA3 Human)

Expression

Bacterial

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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His SUMO TEV R354H SPOP 28-359

Plasmid

#197011

Purpose

E. coli expression vector

Depositor

Tanja Mittag

Article

Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

Insert

SPOP (Spop Human)

Tags

6His-SUMO-TEV

Expression

Bacterial

Mutation

R354H

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_rSERT_C109A_Y107C

Plasmid

#190174

Purpose

For expression of a SERT mutant in mammalian cells and measuring accessibility in the SERT extracellular pathway

Depositor

Gary Rudnick

Article

Jacobs et al J Biol Chem. 2007 Oct 5;282(40):29441-7.

Insert

SLC6A4 Serotonin Transporter (Slc6a4 Rat)

Tags

FLAG, His6, and c-myc

Expression

Mammalian

Mutation

changed Cys109 to alanine, changed tyr107 to cyst…

Promoter

CMV

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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TTR C10S S85C G83R

Plasmid

#184747

Purpose

bacterial expression of human TTR mutants

Depositor

Peter Wright

Article

Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

C10S, S85C, G83R

Promoter

T7

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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1N/2CxNLS-cMyc-NLP-cMyc enAspCas12a

Plasmid

#182126

Purpose

pET21a protein expression vector for 1N/2CxNLS-cMyc-NLP-cMyc enAspCas12a in bacteria

Depositor

Scot Wolfe

Article

Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

Insert

1N/2CxNLS-cMyc-NLP-cMyc AspCas12aen

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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1N/2CxNLS-cMyc-NLP-cMyc AspCas12a

Plasmid

#182121

Purpose

pET21a protein expression vector for 1N/2CxNLS-cMyc-NLP-cMyc AspCas12a in bacteria

Depositor

Scot Wolfe

Article

Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

Insert

1N/2CxNLS-cMyc-NLP-cMyc AspCas12a

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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1N/2CxNLS-cMyc-NLP-cMyc LbaCas12a

Plasmid

#182124

Purpose

pET21a protein expression vector for 1N/2CxNLS-cMyc-NLP-cMyc LbaCas12a in bacteria

Depositor

Scot Wolfe

Article

Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

Insert

1N/2CxNLS-cMyc-NLP-cMyc LbaCas12a

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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TTR C10S S85C Y78F

Plasmid

#184746

Purpose

bacterial expression of human TTR mutants

Depositor

Peter Wright

Article

Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

C10S, S85C, Y78F

Promoter

T7

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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