We narrowed to 11,368 results for: VARS

1. cpLOV2-V7

   Plasmid

   #168993

   Purpose

   circularly permuted LOV2 variant-V7

   Depositor

   Yubin Zhou

   Article

   He et al Nat Chem Biol. 2021 Aug;17(8):915-923. d

   Insert

   cpLOV2-V7
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL2_167

   Plasmid

   #199099

   Purpose

   Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

   Depositor

   Charlie Gilbert, Nili Ostrov, Cultivarium Tools

   Article

   Gilbert et al bioRxiv 2023.05.19.541510

   Insert

   pBBR1 ORI plus NGS barcode 13, gentamicin resistance cassette, mScarlet-I cassette, oriT
   

   Use

   Synthetic Biology

   Available Since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL2_170

   Plasmid

   #199102

   Purpose

   Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

   Depositor

   Charlie Gilbert, Nili Ostrov, Cultivarium Tools

   Article

   Gilbert et al bioRxiv 2023.05.19.541510

   Insert

   pSa ORI plus NGS barcode 16, gentamicin resistance cassette, mScarlet-I cassette, oriT
   

   Use

   Synthetic Biology

   Available Since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDEST14-DoubleCatcher-⍺-Lock

   Plasmid

   #216286

   Purpose

   Expresses DoubleCatcher-⍺-Lock in bacterial cells. DoubleCatcher-⍺-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   DoubleCatcher ⍺-Lock
   

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST14-DoubleCatcher-β-Lock

   Plasmid

   #216287

   Purpose

   Expresses DoubleCatcher-β-Lock in bacterial cells. DoubleCatcher-β-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   DoubleCatcher β-Lock
   

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDEST14-DoubleCatcher-γ-Lock

   Plasmid

   #216288

   Purpose

   Expresses DoubleCatcher-γ-Lock in bacterial cells. DoubleCatcher-γ-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   DoubleCatcher γ-Lock
   

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDEST14-DoubleCatcher-δ-Lock

   Plasmid

   #216289

   Purpose

   Expresses DoubleCatcher-δ-Lock in bacterial cells. DoubleCatcher-δ-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   DoubleCatcher δ-Lock
   

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDEST14-DoubleCatcher-ε-Lock

   Plasmid

   #216290

   Purpose

   Expresses DoubleCatcher-ε-Lock in bacterial cells. DoubleCatcher-ε-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   DoubleCatcher ε-Lock
   

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTlpA-Wasabi-NF

   Plasmid

   #86100

   Purpose

   Nonfluorescent variant of mWasabi reporter gene gated by wild type TlpA

   Depositor

   Mikhail Shapiro

   Article

   Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

   Inserts

   TlpA
   mWasabi
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   D341A and S71T, G73A

   Promoter

   TlpA and TlpA, LacI

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. LENG

    Plasmid

    #111162

    Purpose

    miR-E (miR-30 variant)-based RNAi with GFP reporter

    Depositor

    Johannes Zuber

    Article

    Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    GFP

    Available Since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. eNme2C-CBE6a

    Plasmid

    #215829

    Purpose

    Express CBE6a (with eNme2-C variant) in mammalian cells

    Depositor

    David Liu

    Article

    Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

    Insert

    CBE6a-eNme2C-2xUGI
    

    Expression

    Mammalian

    Available Since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDB007ns2a

    Plasmid

    #99214

    Purpose

    Accessory plasmid for PACE of PylRS variants; negative selection

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); Ptet [SD4] T7RNAP(S12*,S203*)
    

    Mutation

    amber codons at positions 12 and 203 of T7 RNA po…

    Promoter

    Ppsp, PproK, and Ptet

    Available Since

    Oct. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDB038a

    Plasmid

    #99211

    Purpose

    Accessory plasmid for PACE of PylRS variants

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Ppsp [SD8] gIII(P29*,Y184*), luxAB; PproK pylT
    

    Expression

    Bacterial

    Mutation

    amber codons at positions 29 and 184 of gIII

    Promoter

    Ppsp and PproK

    Available Since

    Oct. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. GFP-Paxillin DN

    Plasmid

    #186153

    Purpose

    For expressing paxillin variants in mammalian cells

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    PXN (PXN Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Paxilln residues 1-321 added to N-terminus of WT …

    Promoter

    cmv

    Available Since

    July 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYC56

    Plasmid

    #63910

    Purpose

    Recipient integrative vector to construct mCherry tagged fusions

    Depositor

    Irene Castano Navarro

    Article

    Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

    Type

    Empty backbone

    Tags

    mCherry

    Expression

    Yeast

    Available Since

    April 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDisplay-Gncp-iGluSnFR

    Plasmid

    #107337

    Purpose

    Noncircularly permutated (ncp) variant of iGluSnFR. Membrane-displayed.

    Depositor

    Robert Campbell

    Article

    Wu et al ACS Chem Biol. 2018 Jan 22. doi: 10.1021

    Insert

    GltI based and noncircularly permutated glutamate biosensor
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDB038b

    Plasmid

    #99212

    Purpose

    Accessory plasmid for PACE of PylRS variants

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Ppsp [SD8] gIII(P29*,P83*,Y184*), luxAB; PproK pylT
    

    Expression

    Bacterial

    Mutation

    amber codons at positions 29, 83, and 184 of gIII

    Promoter

    Ppsp and PproK

    Available Since

    Oct. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGL2_176

    Plasmid

    #199108

    Purpose

    Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

    Depositor

    Charlie Gilbert, Nili Ostrov, Cultivarium Tools

    Article

    Gilbert et al bioRxiv 2023.05.19.541510

    Insert

    p15A ORI plus NGS barcode 24, gentamicin resistance cassette, mScarlet-I cassette, oriT
    

    Use

    Synthetic Biology

    Available Since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGL2_169

    Plasmid

    #199101

    Purpose

    Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

    Depositor

    Charlie Gilbert, Nili Ostrov, Cultivarium Tools

    Article

    Gilbert et al bioRxiv 2023.05.19.541510

    Insert

    pIJ101 ORI plus NGS barcode 15, gentamicin resistance cassette, mScarlet-I cassette, oriT
    

    Use

    Synthetic Biology

    Available Since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. CMV-mito-CitroffRH

    Plasmid

    #158548

    Purpose

    Mitochondria-targeted nonbinding variant of inverse-response citrate sensor

    Depositor

    Robert Campbell

    Article

    Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

    Insert

    CitroffRH
    

    Expression

    Mammalian

    Promoter

    cmv

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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