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pCMV G-CEPIA1er

Plasmid

#58215

Purpose

Green fluorescent indicator for calcium imaging in the endoplasmic reticulum

Depositor

Masamitsu Iino

Article

Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

Insert

G-CEPIA1er

Tags

myc

Expression

Mammalian

Mutation

G-GECO1.1 E299D M304L D328N N345K F360W G369S E37…

Promoter

CMV

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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SPLICS LY-MT Short P2A

Plasmid

#213614

Purpose

Detect the short-range Lysosome-Mitochondria contact

Depositor

Tito Calì

Article

Giamogante et al Nat Commun. 2024 Feb 19;15(1):1516. doi:

Insert

SPLICS LY-MT Short P2A

Expression

Mammalian

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcw107

Plasmid

#62511

Purpose

Gateway Expression Vector

Depositor

John Doench, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Jan. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pET-NLS-Cas9-6xHis

Plasmid

#62934

Purpose

Expression of NLS-Cas9-6xHis in bacterial cells

Depositor

David Liu

Article

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

NLS-Cas9-6xHis

Tags

6xHis tag and NLS

Expression

Bacterial

Promoter

T7

Available Since

May 11, 2015

Availability

Academic Institutions and Nonprofits only

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pCI-syn-fRGECO1

Plasmid

#125243

Purpose

Expresses red fluorescent calcium sensor in hippocampal slices

Depositor

Katalin Torok

Article

Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

Insert

fRGECO1

Expression

Mammalian

Mutation

D323A

Promoter

synapsin

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCRISPRa_all-in-one

Plasmid

#183695

Purpose

Expresses CRISPRa components including dCas9-VP64, Synergistic Activation Mediators (SAM), mCherry, and gRNA expression cassette, blasticidin selective

Depositor

Edward Avezov

Article

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Type

Empty backbone

Use

CRISPR

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-ATF6

Plasmid

#32955

Purpose

For visualization of GFP-ATF6 in mammalian cells.

Depositor

Ron Prywes

Article

Chen et al J Biol Chem. 2002 Apr 12. 277(15):13045-

Insert

ATF6 (ATF6 Human)

Tags

EGFP

Expression

Mammalian

Mutation

M67L and M313I

Promoter

CMV

Available Since

Nov. 7, 2011

Availability

Academic Institutions and Nonprofits only

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pT22-GluCNZ-6His

Plasmid

#213504

Purpose

Designed for cytoplasmic E.coli overexpression of glucanase (β-1,3-glycosidase) for gentle yeast spheroblasting that unlike Zymolyase prevents proteolysis of intracellular components.

Depositor

John Denu

Article

Kuznetsov et al J Biol Chem. 2018 Aug 3;293(31):12271-12

Insert

Glucanase (glucan endo-1,3-beta-glucosidase)

Tags

6His tag

Expression

Bacterial

Mutation

1-36 Signal Sequence Deletion

Promoter

T7

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCIG3 (pCMV-IRES-GFP version 3)

Plasmid

#78264

Purpose

Lentiviral empty cloning vector containing IRES and GFP

Depositor

Felicia Goodrum

Article

Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Tags

IRES-EGFP

Expression

Mammalian

Promoter

CMV, lentiviral LTR

Available Since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pMALcPP-MS2cp

Plasmid

#203351

Purpose

Expresses His/MBP-tagged MS2-CP V75E/A81G variant in bacteria

Depositor

Thomas Cech

Article

Hemphill et al Proc Natl Acad Sci U S A. 2023 Jun 27;12

Insert

MS2 coat protein

Tags

Hx6-MBP

Expression

Bacterial

Mutation

V75E and A81G, to prevent capsid assembly

Available Since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

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V246 pCEP 4HA B56beta

Plasmid

#14533

Depositor

David Virshup

Article

Seeling et al Science. 1999 Mar 26. 283(5410):2089-91.

Insert

PPP2R5B B56 beta regulatory subunit of PP2A (PPP2R5B Human)

Tags

4 HA

Expression

Mammalian

Mutation

I346V relative to GenBank ID L42374

Available Since

March 23, 2007

Availability

Academic Institutions and Nonprofits only

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pGL3-U6-sgRNA-PGK-puromycin

Plasmid

#51133

Purpose

expresses sgRNA from the U6 promoter.

Depositor

Xingxu Huang

Article

Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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hSTARR-seq_ORI vector

Plasmid

#99296

Purpose

Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

Depositor

Alexander Stark

Article

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Type

Empty backbone

Use

Hstarr-seq screening vector

Expression

Mammalian

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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N8his-GFPenhancer-GGGGS4-LaG16

Plasmid

#140442

Purpose

We designed a flexible linker to connect two types of anti GFP nanobodies and used this fusion nanobody to purify GFP tagged protein.

Depositor

Motoyuki Hattori

Article

Zhang et al Sci Rep. 2020 Apr 10;10(1):6239. doi: 10

Insert

GFPenhancer-GGGGS4-LaG16

Tags

8 histidine tag

Expression

Bacterial

Promoter

T7 promotor

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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SPLICS LY-MT Long P2A

Plasmid

#213613

Purpose

Detect the long-range Lysosome-Mitochondria contact

Depositor

Tito Calì

Article

Giamogante et al Nat Commun. 2024 Feb 19;15(1):1516. doi:

Insert

SPLICS LY-MT Long P2A

Expression

Mammalian

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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SPLICS PM-ER Long P2A

Plasmid

#164111

Purpose

Detect the long Plasma membrane-Endoplasmic reticulum contact

Depositor

Marisa Brini, Tito Calì

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split YFP PM-ER Long P2A

Expression

Mammalian

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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scFLARE2

Plasmid

#158700

Purpose

Neuronal expression of scFLARE2 component

Depositor

Alice Ting

Article

Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

Insert

scFLARE2

Use

AAV

Tags

HA, Flag, VP16

Expression

Mammalian

Mutation

Truncated form of TEV protease (220-242 amino aci…

Promoter

Syn

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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SPLICS PM-ER Short P2A

Plasmid

#164112

Purpose

Detect the short Plasma membrane-Endoplasmic reticulum contact

Depositor

Marisa Brini, Tito Calì

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split YFP PM-ER Short P2A

Expression

Mammalian

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-Dcp1a

Plasmid

#153972

Purpose

Fluorescent protein fused to mRNA decapping enzyme 1a

Depositor

Gia Voeltz

Article

Lee et al Science. 2020 Jan 31;367(6477). pii: 367

Insert

mRNA decapping enzyme 1a (DCP1A Human)

Tags

pAcGFP-C1

Expression

Mammalian

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pUC57-sgRNA expression vector

Plasmid

#51132

Purpose

For in vitro transcription of sgRNA from the T7 promoter.

Depositor

Xingxu Huang

Article

Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

T7

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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