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pHAGE2-TetO-Salhel-II-Sox2HMG

Plasmid

#231534

Purpose

Expresses chimeric Sox2 HMG in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

mSox2 HMG flanked with N- and C-termini of Salhel-II

Use

Lentiviral

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-Chim-Salhel-I

Plasmid

#231513

Purpose

Expresses Salpingoeca helianthica chimeric Sox-I in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Salhel-I HMG flanked with N- and C-termini of mSox2

Use

Lentiviral

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-Salhel-POU

Plasmid

#231522

Purpose

Expresses Salpingoeca helianthica full-length POU in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

POU

Use

Lentiviral

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-Salhel-Sox-II

Plasmid

#231516

Purpose

Expresses Salpingoeca helianthica full-length Sox-II in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Sox-II

Use

Lentiviral

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-Myflu-Sox-II

Plasmid

#231518

Purpose

Expresses Mylnosiga fluctuans full-length Sox-II in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Sox-II

Use

Lentiviral

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-Chim-Pchi

Plasmid

#231514

Purpose

Expresses Pigoraptor chileana chimeric Sox in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Pchi HMG flanked with N- and C-termini of mSox2

Use

Lentiviral

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-Myflu-Sox-I

Plasmid

#231517

Purpose

Expresses Mylnosiga fluctuans full-length Sox-I in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Sox-I

Use

Lentiviral

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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SCML2_3

Plasmid

#220878

Purpose

Baculovirus expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Article

Nady et al J Mol Biol. 2012 Nov 9;423(5):702-18. do

Insert

SCML2 (SCML2 Human)

Tags

6xHis-TEV

Expression

Bacterial

Promoter

T7

Available Since

June 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pMX_mePOU

Plasmid

#206396

Purpose

Expresses mouse ePOU in mammalian cells, for retrovirus generation.

Depositor

Ralf Jauch

Article

Tan et al Mol Biol Evol. 2021 Jun 25;38(7):2854-28

Insert

ePOU

Use

Retroviral

Expression

Mammalian

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pLVTHM-mSox17FNV-3xflag

Plasmid

#206375

Purpose

Expresses 3xflag fused mouse SOX17FNV in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Article

Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

Insert

Sox17FNV-3xflag (Sox17 Mouse)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Available Since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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rtTA-sg-CMV152

Plasmid

#188485

Purpose

gRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 152.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

CMV promoter sgRNA

Use

CRISPR

Expression

Mammalian

Mutation

None

Promoter

U6

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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rtTA-sg-CMV162

Plasmid

#188486

Purpose

gRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 162.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

CMV promoter sgRNA

Use

CRISPR

Expression

Mammalian

Mutation

None

Promoter

U6

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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FAK Fus

Plasmid

#186145

Purpose

For expression of recombinant FAK using baculovirus in SF9 cells

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

PTK2 (PTK2 Mouse)

Tags

His6

Expression

Insect

Mutation

C-terminal linker swapped with FUS IDR

Promoter

polyhedrin promoter

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pFastBac FAK W266A

Plasmid

#186144

Purpose

For expresseion of recombinant FAK using baccolovirus in SF9 cells

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

PTK2 (PTK2 Mouse)

Tags

His6

Expression

Insect

Mutation

DNA mutation T796G;G797C Amino acid mutation W266A

Promoter

polyhedrin promoter

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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FAK Pyk2

Plasmid

#186146

Purpose

For expresseion of recombinant FAK using baccolovirus in SF9 cells

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

PTK2 (PTK2 Mouse)

Tags

His6

Expression

Insect

Mutation

C-terminal linker swapped with Pyk2 linker

Promoter

polyhedrin promoter

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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hCMV-IE1:RoLuc:bgH

Plasmid

#135290

Purpose

Transcriptional unit for constitutive expression of RoLuc in mammalian cells

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

CMV:RoLuc:bGH

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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hCMV-IE1:MetLuc:bgH

Plasmid

#135292

Purpose

Transcriptional unit for constitutive expression of MetLuc in mammalian cells

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

CMV:MetLuc:bGH

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-sg-CMV152

Plasmid

#179916

Purpose

All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 152.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

CMV promoter sgRNA

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Promoter

U6

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-sg-CMV51

Plasmid

#179915

Purpose

All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 51.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

CMV promoter sgRNA

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Promoter

U6

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pX459-sg-CMV162

Plasmid

#179917

Purpose

All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 162.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

CMV promoter sgRNA

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Promoter

U6

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge