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pLL3.7-EF-EYFP-YAP1_R89A-PolyA

Plasmid

#112291

Purpose

Fluorescently labels human YAP1-2gamma isoform (504 aa) R89A mutant

Depositor

Erik Sahai

Article

Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

Insert

EYFP-YAP1 (YAP1 Human)

Use

Lentiviral

Mutation

Human YAP1-2gamma with Arginine 89 to Alanine

Promoter

EF-1 alpha

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pLL3.7-EF-EYFP-YAP1_L91A-PolyA

Plasmid

#112292

Purpose

Fluorescently labels human YAP1-2gamma isoform (504 aa) L91A mutant

Depositor

Erik Sahai

Article

Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

Insert

EYFP-YAP1 (YAP1 Human)

Use

Lentiviral

Mutation

Human YAP1-2gamma with Leucine 91 to Alanine

Promoter

EF-1 alpha

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pLL3.7-EF-EYFP-YAP1_F95A-PolyA

Plasmid

#112293

Purpose

Fluorescently labels human YAP1-2gamma isoform (504 aa) F95A mutant

Depositor

Erik Sahai

Article

Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

Insert

EYFP-YAP1 (YAP1 Human)

Use

Lentiviral

Mutation

Human YAP1-2gamma with Phenylalanine 95 to Alanine

Promoter

EF-1 alpha

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pDONOR-SNCAe3-A53T

Plasmid

#85848

Purpose

Donor plasmid for SNCA exon3 A53T sequence. Also contains EGFP and tagBFP

Depositor

Jens Schwamborn

Article

Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

Insert

SNCA exon 3 homology arms (SNCA Human)

Expression

Bacterial and Mammalian

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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ACA8-mYFP

Plasmid

#87245

Purpose

fluorescent labelling of ACA8

Depositor

Silke Robatzek

Article

Frei dit Frey et al Plant Physiol. 2012 Jun;159(2):798-809.

Insert

ACA8 (ACA8 Mustard Weed)

Tags

YFP

Promoter

35s

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pGAPTrap-Clover-Muro

Plasmid

#82334

Purpose

Vector targeting the Clover gene to the GAPDH locus of human cells. Clover is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.

Depositor

Ed Stanley

Article

Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

Insert

Clover-T2AMuro

Use

Synthetic Biology

Expression

Mammalian

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pGAPTrap-eGFP-IRESMeo

Plasmid

#82502

Purpose

Vector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH. G418 resistance is encoded by the Meo gene.

Depositor

Ed Stanley

Article

Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

Insert

eGFP-IRESMeo

Use

Synthetic Biology

Expression

Mammalian

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pde3b_L (OZ591)

Plasmid

#35231

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting pde3b (pde3b Zebrafish)

Use

Zebrafish targeting

Available Since

March 16, 2012

Availability

Academic Institutions and Nonprofits only
pde3b_R (OZ592)

Plasmid

#35232

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting pde3b (pde3b Zebrafish)

Use

Zebrafish targeting

Available Since

March 15, 2012

Availability

Academic Institutions and Nonprofits only
LOC566040_ R (OZ588)

Plasmid

#35228

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting LOC566040 (LOC566040 Zebrafish)

Use

Zebrafish targeting

Available Since

March 15, 2012

Availability

Academic Institutions and Nonprofits only
fam82a2_L (OZ573)

Plasmid

#35213

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting fam82a2 (rmdn3 Zebrafish)

Use

Zebrafish targeting

Available Since

March 15, 2012

Availability

Academic Institutions and Nonprofits only
LOC566040_ L (OZ587)

Plasmid

#35227

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting LOC566040 (LOC566040 Zebrafish)

Use

Zebrafish targeting

Available Since

March 5, 2012

Availability

Academic Institutions and Nonprofits only
morc3b_R (OZ576)

Plasmid

#35216

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting morc3b (morc3b Zebrafish)

Use

Zebrafish targeting

Available Since

March 5, 2012

Availability

Academic Institutions and Nonprofits only
fam82a2_R (OZ574)

Plasmid

#35214

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting fam82a2 (rmdn3 Zebrafish)

Use

Zebrafish targeting

Available Since

March 5, 2012

Availability

Academic Institutions and Nonprofits only
morc3b_L (OZ575)

Plasmid

#35215

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting morc3b (morc3b Zebrafish)

Use

Zebrafish targeting

Available Since

March 5, 2012

Availability

Academic Institutions and Nonprofits only
pCMV-Tag4A-MMP8 (E87D)

Plasmid

#29547

Depositor

Yardena Samuels

Article

Palavalli et al Nat Genet. 2009 May . 41(5):518-20.

Insert

Metalloproteinase protein 8 (MMP8 Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

E87D

Available Since

Sept. 16, 2011

Availability

Academic Institutions and Nonprofits only
pEMS1710

Plasmid

#29296

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).

Depositor

Elizabeth Simpson

Article

de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

Insert

Ple238 (TRH Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Available Since

June 10, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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xylt1_R (OZ510)

Plasmid

#27185

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting xylt1 (xylt1 Zebrafish)

Use

Zebrafish targeting

Available Since

Feb. 8, 2011

Availability

Academic Institutions and Nonprofits only
pitrm1_R (OZ516)

Plasmid

#27191

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting pitrm1 (pitrm1 Zebrafish)

Use

Zebrafish targeting

Available Since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only
pLenti CMV Hygro dn-cHSF1

Plasmid

#134732

Purpose

Lentiviral expression vector for constitutively active dominant-negative HSF1

Depositor

Matthew D. Shoulders

Article

Moore et al ACS Chem Biol. 2016 Jan 15;11(1):200-10.

Insert

dn-cHSF1 (HSF1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Deletion of amino acids 186-202, 379−529

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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