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pST39-Mega'-deltaGJ

Plasmid

#216927

Purpose

Mega' with knockout of GvpG and GvpJ

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

Mega' with knockout of GvpG and GvpJ

Expression

Bacterial

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pST39-Mega'-deltaTU

Plasmid

#216944

Purpose

Mega' with knockout of GvpT and GvpU

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

Mega' with knockout of GvpT and GvpU

Expression

Bacterial

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1(S131C)

Plasmid

#224744

Purpose

In vitro transcription for Oocyte expression of mTREK-1(S131C)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Mutation

S131C

Promoter

T7

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1(S131A)

Plasmid

#224745

Purpose

In vitro transcription for Oocyte expression of mTREK-1(S131A)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Mutation

S131A

Promoter

T7

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1(G171F)

Plasmid

#224747

Purpose

In vitro transcription for Oocyte expression of mTREK-1(G171F)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Mutation

G171F

Promoter

T7

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1(A286F)

Plasmid

#224746

Purpose

In vitro transcription for Oocyte expression of mTREK-1(A286F)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Mutation

A286F

Promoter

T7

Available Since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pKL1-100

Plasmid

#219408

Purpose

Reporter gene (4 operators) for zinc finger activator

Depositor

Ahmad Khalil

Article

Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.

Insert

(42-10 op)x4-minCyc1-Venus-tCyc1

Expression

Yeast

Available Since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

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pKL1-422

Plasmid

#219410

Purpose

Reporter gene (1 operator) for zinc finger activator

Depositor

Ahmad Khalil

Article

Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.

Insert

(42-10 op)x1-minCyc1-Venus-tCyc1

Expression

Yeast

Available Since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

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pKL1-424

Plasmid

#219412

Purpose

Reporter gene (3 operator) for zinc finger activator

Depositor

Ahmad Khalil

Article

Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.

Insert

(42-10 op)x3-minCyc1-Venus-tCyc1

Expression

Yeast

Available Since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

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pKL1-095

Plasmid

#219407

Purpose

Reporter gene (4 operators) for zinc finger activator

Depositor

Ahmad Khalil

Article

Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.

Insert

(13-6 op)x4-minCyc1-Venus-tCyc1

Expression

Yeast

Available Since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

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pKL1-423

Plasmid

#219411

Purpose

Reporter gene (2 operator) for zinc finger activator

Depositor

Ahmad Khalil

Article

Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.

Insert

(42-10 op)x2-minCyc1-Venus-tCyc1

Expression

Yeast

Available Since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

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TRAPa(R150A)-3xFLAG-pCDNA

Plasmid

#201966

Purpose

Mammalian expression of mutated Translocon-associated protein subunit alpha

Depositor

Ville Paavilainen

Article

Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

Insert

SSR1 (SSR1 Human)

Tags

3xFLAG-tag

Expression

Mammalian

Mutation

R150A

Available Since

Jan. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pMx/ATP10A(E203Q)-HA

Plasmid

#209258

Purpose

Mammalian expression of ATP10A mutant E203Q

Depositor

Hye-Won Shin

Article

Naito et al J Biol Chem. 2015 Jun 12;290(24):15004-1

Insert

ATP10A (ATP10A Human)

Use

Retroviral

Tags

HA

Mutation

E203Q

Available Since

Nov. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pHR- flag-Lag43-iCAM-tether

Plasmid

#205211

Purpose

This vector encodes of the synCAM tether (iCAM1) and GFP nanobody LAG43

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM iCAM1 tether and Lag43 anti-GFP nanobody

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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PH-PLCd1-mNGx2

Plasmid

#202752

Purpose

expresses fluorescent protein-tagged lipid biosensor

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

PLCD1v2(1-170): mNG2:mNG2 (PLCD1 Human)

Expression

Mammalian

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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sgRNA1 _8xCTS-ECFP-pA

Plasmid

#200243

Purpose

Mammalian transfections; 8xCTS-ECFP reporter construct 1

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Insert

sgRNA1_8xCTS

Expression

Mammalian

Available Since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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sgRNA1 _1xCTS-ECFP-pA

Plasmid

#200237

Purpose

Mammalian transfections; 1xCTS-ECFP reporter construct 1

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Insert

sgRNA1_1xCTS

Expression

Mammalian

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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sgRNA5 _1xCTS-ECFP-pA

Plasmid

#200241

Purpose

Mammalian transfections; 1xCTS-ECFP reporter construct 5

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Insert

sgRNA5_1xCTS

Expression

Mammalian

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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sgRNA3 _1xCTS-ECFP-pA

Plasmid

#200239

Purpose

Mammalian transfections; 1xCTS-ECFP reporter construct 3

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Insert

sgRNA3_1xCTS

Expression

Mammalian

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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gRNA4 _8xCTS-ECFP-pA

Plasmid

#200246

Purpose

Mammalian transfections; 8xCTS-ECFP reporter construct 4

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Insert

sgRNA4_8xCTS

Expression

Mammalian

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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