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R791-X15-636: His6-MBP-tev-Hs.NF1 DEF (1541-2818)

Plasmid

#159578

Purpose

Baculovirus protein expression of His6-MBP-tev-Hs.NF1 DEF (1541-2818)

Depositor

Dominic Esposito

Article

Sherekar et al J Biol Chem. 2020 Jan 24;295(4):1105-111

Insert

His6-MBP-tev-Hs.NF1 DEF (1541-2818) (NF1 Human)

Use

Tags

His6-MBP-tev

Expression

Insect

Mutation

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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Control-cx43.4-48H-pPRISM

Plasmid

#117801

Purpose

Control to test technique on a target that is known to work

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pPRISM-TagRFP-cmlc2-mRFP

Plasmid

#117769

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pPRISM-Fuse-gcry-eGFP

Plasmid

#117785

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

mRFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
EphA2 430-976 pcDNA3.1

Plasmid

#102738

Purpose

Expression of c terminal EphA2 in mammalian cells

Depositor

Jin Chen

Article

Amato et al Cancer Res. 2016 Jan 15;76(2):305-18. do

Insert

EphA2 N terminal (430-976aa) (Epha2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pPRISM-TagRFP-cmlc2-eGFP

Plasmid

#117770

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pPRISM-Fuse-gcry-mRFP

Plasmid

#117784

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

mRFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pPRISM-Cre-gcry-mRFP

Plasmid

#117790

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

Cre

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
HH06-LP Clone 6 heavy chain

Plasmid

#192176

Purpose

Clone 6 heavy chain (HH06)

Depositor

Sidi Chen

Article

Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

Insert

Clone 6 heavy chain (HH06)

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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HH02-LP Clone 2 heavy chain

Plasmid

#192175

Purpose

Clone 2 heavy chain (HH02)

Depositor

Sidi Chen

Article

Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

Insert

Clone 2 heavy chain (HH02)

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
HK02-LP Clone 2 light chain

Plasmid

#192179

Purpose

Clone 2 light chain (HK02)

Depositor

Sidi Chen

Article

Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

Insert

Clone 2 light chain (HK02)

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pPRISM-Cre-cmlc2-mRFP

Plasmid

#117793

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

Cre

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pPRISM-Fuse-gcry-TagBFP

Plasmid

#117783

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

mRFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pPRISM-Cre-gcry-TagBFP

Plasmid

#117789

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

Cre

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pPRISM-Cre-cmlc2-TagBFP

Plasmid

#117792

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

Cre

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pPRISM-Fuse-cmlc2-eGFP

Plasmid

#117788

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

mRFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pTrcHisB mLCAD (29-430 aa)

Plasmid

#24490

Depositor

Eric Verdin

Article

Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

Insert

Long chain acyl-CoA dehydrogenase (Acadl Mouse)

Use

Tags

6xHis

Expression

Bacterial

Mutation

aa 1-28 deleted encodes active 29-430

Promoter

Available since

May 3, 2010

Availability

Academic Institutions and Nonprofits only
EphA2 1-427 pcDNA3.1

Plasmid

#102737

Purpose

Expression of N terminal EphA2 in mammalian cells

Depositor

Jin Chen

Article

Amato et al Cancer Res. 2016 Jan 15;76(2):305-18. do

Insert

EphA2 N terminal (1-427aa) (Epha2 Mouse)

Use

Tags

6xHis and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1mLCAD_8KR_FLAG

Plasmid

#24485

Depositor

Eric Verdin

Article

Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

Insert

Long chain acyl-CoA dehydrogenase (Acadl Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Lys42, 156, 189, 240, 254, 318, 322, 358Arg (8KR)

Promoter

Available since

April 30, 2010

Availability

Academic Institutions and Nonprofits only
pHAGE2-EF1a-E1MLS-2×FLAG-T4Lig-IRES-tdTomato

Plasmid

#234952

Purpose

Human codon optimized T4 DNA Ligase with N-terminal E1 MLS expressing plasmid

Depositor

Agnel Sfeir

Article

Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

human codon optimized T4 DNA Ligase with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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