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pSF3-Flag-FRB-Cbir_FKBP-Myc-Nbir

Plasmid

#90009

Purpose

Split-BioID plasmid: Expresses C-terminally tagged CBir[257-321]-FRB and C-terminally tagged NBir[2-256]-FKBP

Depositor

Julien Béthune

Article

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

FKBP-linker-Myc-NBirA*
FRB-linker-CBirA*

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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pV1524

Plasmid

#111431

Purpose

CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Mal2-FLP)

Depositor

Gerald Fink

Article

Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

Insert

CaCas9/sgRNA-BsmBI stuffer

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-fDIO-mNeonGreen

Plasmid

#99133

Purpose

An AAV genome with Flp recombinase-dependent expression of mNeonGreen from the CAG promoter

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Has Service

AAV1

Insert

fDIO-mNeonGreen

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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pSF3-Flag-CBir-FRB_Myc-NBir-FKBP

Plasmid

#90003

Purpose

Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-FRB and N-terminally tagged NBir[2-256]-FKBP

Depositor

Julien Béthune

Article

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

NBirA*-linker-FKBP
CBirA*-linker-FRB

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn1-fDIO-mScarlet-KASH-2a-dCre

Plasmid

#203839

Purpose

Flp-dependent enzymatically inactive Cre and mScarlet-KASH expression

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

dCre

Use

AAV

Tags

mScarlet-KASH-2A

Expression

Mutation

Enzymatically inactive Cre

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry

Plasmid

#75470

Purpose

Flp-dependent Chr2-mCherry encoded in an AAV vector for rAAV production and expression in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

Has Service

AAV1

Insert

Reversed ChR2(H134R)-mCherry

Use

AAV and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn FLEx-FRT hM3Dq-mCherry

Plasmid

#161575

Purpose

Expression of hM3Dq-mCherry in a Flp-dependent fashion

Depositor

Kevin Beier, Robert Malenka

Article

Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

Insert

hM3Dq-mCherry

Use

AAV

Tags

Expression

Mutation

NA

Promoter

hSyn

Available since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pFRT-TO-DEST

Plasmid

#106348

Purpose

Destination vector for gateway cloning. Developed for Flp-In T-REx system.

Depositor

Thomas Tuschl

Article

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Type

Empty backbone

Use

Gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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pSF3-Flag-FRB-Cbir_Myc-NBir-FKBP

Plasmid

#90008

Purpose

Split-BioID plasmid: Expresses C-terminally tagged FRB-CBir[257-321] and N-terminally tagged NBir[2-256]-FKBP

Depositor

Julien Béthune

Article

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

NBirA*-linker-FKBP
FRB-linker-CBirA*

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-fDIO-VARNAM2-Kv2.1PR

Plasmid

#195536

Purpose

Flp recombinase-dependent red fluorescent, negative response-polarity voltage indicator; soma-targeted

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

VARNAM2-Kv2.1 proximal restriction sequence

Use

AAV

Tags

Expression

Mammalian

Mutation

VARNAM SI linker

Promoter

CAG

Available since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pSF3-Flag-CBir-FRB_FKBP-Myc-Nbir

Plasmid

#90004

Purpose

Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-FRB and C-terminally tagged FKBP-NBir[2-256]

Depositor

Julien Béthune

Article

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

FKBP-linker-Myc-NBirA*
CBirA*-linker-FRB

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn FLEx-FRT hM4Di-mCherry

Plasmid

#161576

Purpose

Expression of hM4Di-mCherry in a Flp-dependent fashion

Depositor

Kevin Beier, Robert Malenka

Article

Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

Insert

hM4Di-mCherry

Use

AAV

Tags

Expression

Mutation

NA

Promoter

hSyn

Available since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pV1393

Plasmid

#111430

Purpose

CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Sap2-FLP)

Depositor

Gerald Fink

Article

Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

Insert

CaCas9/sgRNA-BsmBI stuffer

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-Intron-CterFlag-MePCE

Plasmid

#113549

Purpose

Mammalian expression plasmid that can be used with the Flp-In T-REx system.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

MePCE (MEPCE Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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c3GIC9n

Plasmid

#62192

Purpose

col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9n (D10A variant) expression. Contains NsiI cloning site for U6-sgRNA cassettes

Depositor

Lukas Dow

Article

Dow et al Nat Biotechnol. 2015 Apr;33(4):390-4. do

Insert

humanized S. Pyogenes Cas9 (D10A)

Use

CRISPR

Tags

HA

Expression

Mutation

D10A substitution (Cas9n)

Promoter

TRE3G

Available since

Aug. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pAct-FRT-FRT3-stop-FRT-LexGAD-FRT3-Gal4 attB

Plasmid

#52890

Purpose

"CoinFLP-LexGAD/Gal4" - Expresses LexGAD or Gal4 from actin promoter downsteam of transcriptional stop. Recombination by FLP excises FRT-FRT pair to express LexGAD, or FRT3-FRT3 pair to express Gal4.

Depositor

Iswar Hariharan

Article

Bosch et al Development. 2015 Feb 1;142(3):597-606.

Inserts

LexGAD
Gal4 (GAL4 Budding Yeast)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-mCherry-N

Plasmid

#210271

Purpose

N-terminal mCherry-tagging of inserts for inducible expression. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Type

Empty backbone

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-FDIO-IGK-dAPEX2-KDEL

Plasmid

#117184

Purpose

Plasmid name in publication: pAAV-FDIO-ER-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

Depositor

David Ginty

Article

Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

Insert

IGK-dAPEX2-KDEL

Use

AAV; Flp/frt

Tags

Expression

Mammalian

Mutation

W41F and A134P on soybean APX

Promoter

EF1a

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT TO-EGFP-G3BP1-DeltaRBP

Plasmid

#136005

Purpose

DOX-inducible G3BP1 with RNA binding domains deleted inserted with GFP tagged on the N-terminus and used with the Flp-In T-Rex System

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

G3BP1 (G3BP1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

C-term of G3BP1 Deleted for Delta RBP

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA5-FRT-TO-SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)_P2A_iLID-mCherry-RAB11

Plasmid

#174647

Purpose

Optogenetic coupling of RAB11 to tetramerized moss kinesin-14 to induce retrograde transport of recycling endosomes. Compatible with Flp-in TREX system.

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

SSPB(micro)-mVenus-GCN4-ppKin14Vib(816-1321)-P2A-iLID-mCherry-RAB11

Use

Tags

Expression

Mammalian

Mutation

SSPB:Arg73Gln; mVenus: Met1Del, Thr154Met; ppKin1…

Promoter

CMV (with TetOn)

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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