Skip to main content
Addgene
Showing: 941 - 960 of 15117 results

  1. pPlacPbuCas13b-gRNA-NT

    Plasmid
    #184838
    Purpose
    Expression of single-spacer CRISPR array with a shortened non-targeting spacer for PbuCas13b and expression of PbuCas13b in bacteria.
    Depositor
    Chase Beisel
    Article
    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2
    Insert
    Co-expression of single-spacer CRISPR array with a nontargeting spacer for PbuCas13b and PbuCas13b nuclease in bacteria.
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119 and lac promoter
    Available since
    Oct. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    184838-plasmid-map-sequence-id-373981

    Enlarge

  13. pGreenII 0000 (No LUC)

    Plasmid
    #44465
    Depositor
    Roger Hellens
    Type
    Empty backbone
    Use
    Plant transformation
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 6, 2013
    Availability
    Academic Institutions and Nonprofits only
    44465-plasmid-map-sequence-id-335235

    Enlarge

  14. pAIP4_4QB4

    Plasmid
    #234187
    Purpose
    Heterologous protein expression of thermonuclease (TNase) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4QB4
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234187-plasmid-map-sequence-id-464152

    Enlarge

  15. C13m

    Plasmid
    #120960
    Purpose
    MoClo golden gate assembly CD part for Cerulean (modified CFP; variant of https://www.fpbase.org/protein/cerulean/) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    Cerulean
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120960-plasmid-map-sequence-id-245422

    Enlarge

  16. pHEX2 T494 TALENs

    Plasmid
    #44464
    Depositor
    Ross Johnson, Roger Hellens
    Insert
    AtCRU3 T494 TALENs
    Use
    Plant transformation
    Tags
    Expression
    Mutation
    Promoter
    CaMV 35S
    Available since
    May 6, 2013
    Availability
    Academic Institutions and Nonprofits only

  17. pZP06B

    Plasmid
    #232321
    Purpose
    Make sacB a counterselection marker to replace galK
    Depositor
    Chenggang Wu
    Insert
    PrpsJ-sacB
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    232321-plasmid-map-sequence-id-462085

    Enlarge

  18. pBCG10

    Plasmid
    #232323
    Purpose
    Make mazF a counterselection marker to replace sacB in pZP06C
    Depositor
    Chenggang Wu
    Insert
    mazF-tetR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    232323-plasmid-map-sequence-id-462282

    Enlarge

  19. H2B CFP

    Plasmid
    #198059
    Purpose
    mCerulean fused to human H2B protein
    Depositor
    Amro Hamdoun
    Insert
    H2B
    Use
    Synthetic Biology; Sea urchin, mammal, zebrafish
    Tags
    mCerulean
    Expression
    Mutation
    Promoter
    sp6
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    198059-plasmid-map-sequence-id-389151

    Enlarge

  20. pOpen-T5gene12/13

    Plasmid
    #165559
    Purpose
    Double-stranded DNA specific exonuclease and single-stranded DNA endonuclease. Initiates at the 5' termini of linear or nicked double-stranded DNA. Cleaves linear or nicked double-stranded DNA in the 5' to 3' direction.
    Depositor
    Jennifer Molloy, FreeGenes Project, Drew Endy
    Insert
    T5 Exonuclease
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165559-plasmid-map-sequence-id-339952

    Enlarge

Showing: 941 - 960 of 15117 results