We narrowed to 15,231 results for: LEA

1. pPlacPbuCas13b-gRNA-T-MS2

   Plasmid

   #184839

   Purpose

   Expression of a single-spacer CRISPR array with spacer #1 targeting the MS2 phage genome and expression of PbuCas13b in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

   Insert

   single-spacer CRISPR array with spacer #1 targeting the MS2 phage genome, PbuCas13b nuclease
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119 and lac promoter

   Available since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTE4560

   Plasmid

   #107526

   Purpose

   Expresses As crRNA and mCherry in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Inserts

   As crRNA
   mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and human U6

   Available since

   April 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPlacPbuCas13b-gRNA-T-deGFP

   Plasmid

   #184840

   Purpose

   Expression of a single-spacer CRISPR array with spacer #1 targeting the mRNA of deGFP and expression of PbuCas13b in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

   Insert

   single-spacer CRISPR array with spacer #1 targeting the the mRNA of deGFP, PbuCas13b nuclease
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119 and lac promoter

   Available since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. hFlt1 V767A

   Plasmid

   #86038

   Purpose

   human VEGFR1 with V to A mutation at position 767

   Depositor

   Christie Thomas

   Article

   Raikwar et al Exp Cell Res. 2016 May 15;344(1):103-11.

   Insert

   human Flt1 V767A (FLT1 Human)
   

   Use

   Tags

   FLAG, HA (please see depositor comments below), a…

   Expression

   Mammalian

   Mutation

   V767A

   Promoter

   CMV

   Available since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP-polyQ74-NLS

   Plasmid

   #231892

   Purpose

   Forms nuclear HTT partial exon 1 Q74-NLS aggregates

   Depositor

   Lukas Kapitein

   Article

   Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

   Insert

   HTT partial exon 1 Q74-NLS (HTT Human)
   

   Use

   Tags

   EGFP and SV40 NLS

   Expression

   Mammalian

   Mutation

   CAG repeat expansion

   Promoter

   Available since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPlacPbuCas13b-gRNA-NT

   Plasmid

   #184838

   Purpose

   Expression of single-spacer CRISPR array with a shortened non-targeting spacer for PbuCas13b and expression of PbuCas13b in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

   Insert

   Co-expression of single-spacer CRISPR array with a nontargeting spacer for PbuCas13b and PbuCas13b nuclease in bacteria.
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119 and lac promoter

   Available since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. HaloTag-LaminA

   Plasmid

   #231894

   Purpose

   Expression of HaloTag-tagged LaminA to visualize the nuclear lamina

   Depositor

   Lukas Kapitein

   Article

   Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

   Insert

   LaminA (LMNA Human)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTE4938

   Plasmid

   #107527

   Purpose

   Expresses Fn crRNA and mCherry in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Inserts

   Fn crRNA
   mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and human U6

   Available since

   April 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTE4999

   Plasmid

   #107555

   Purpose

   Expresses human codon optimized inactive FnCpf1(dead1) in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Insert

   hFnCpf1(dead1)
   

   Use

   CRISPR

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   D917A

   Promoter

   CMV

   Available since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTE4567

    Plasmid

    #107557

    Purpose

    Expresses human codon optimized inactive MbCpf1(dead2) in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Insert

    hMbCpf1(dead2)
    

    Use

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    E1080A

    Promoter

    CMV

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTE4561

    Plasmid

    #107525

    Purpose

    Expresses Lb crRNA and mCherry in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    Lb crRNA
    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and human U6

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTE3320

    Plasmid

    #107529

    Purpose

    Expresses human codon optimized LbCpf1(RVR mutant) in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Insert

    hLbCpf1(RVR)
    

    Use

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    G532R, K538V, Y542R

    Promoter

    CMV

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTE3314

    Plasmid

    #107530

    Purpose

    Expresses human codon optimized FnCpf1(RVR mutant) in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Insert

    hFnCpf1(RVR)
    

    Use

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    N607R, K613V, N617R

    Promoter

    CMV

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTE3319

    Plasmid

    #107533

    Purpose

    Expresses human codon optimized MbCpf1(RR mutant) in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Insert

    hMbCpf1(RR)
    

    Use

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    N576R, K637R

    Promoter

    CMV

    Available since

    April 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTE3316

    Plasmid

    #107532

    Purpose

    Expresses human codon optimized FnCpf1(RR mutant) in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Insert

    hFnCpf1(RR)
    

    Use

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    N607R, K671R

    Promoter

    CMV

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTE3317

    Plasmid

    #107531

    Purpose

    Expresses human codon optimized MbCpf1(RVR mutant) in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Insert

    hMbCpf1(RVR)
    

    Use

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    N576R, K582V, N586R

    Promoter

    CMV

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTE3179

    Plasmid

    #107528

    Purpose

    Expresses Mb crRNA and mCherry in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    Mb crRNA
    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and human U6

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGreenII 0000 (No LUC)

    Plasmid

    #44465

    Depositor

    Roger Hellens

    Type

    Empty backbone

    Use

    Plant transformation

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGreenII 0579-1 (35S::LUC)

    Plasmid

    #44468

    Depositor

    Roger Hellens

    Insert

    A luciferase gene
    

    Use

    Plant transformation

    Tags

    Expression

    Mutation

    Promoter

    35S Promoter

    Available since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAIP4_4QB4

    Plasmid

    #234187

    Purpose

    Heterologous protein expression of thermonuclease (TNase) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    4QB4
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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