We narrowed to 5,332 results for: pcdna3.1

1. pcDNA3.1-Renilla-ccdB

   Plasmid

   #87068

   Purpose

   Mammalian Gateway-compatible expression vector backbone with an N-terminal Renilla luciferase

   Depositor

   Mikko Taipale

   Type

   Empty backbone

   Tags

   Renilla luciferase

   Expression

   Mammalian

   Available Since

   March 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1 PA-mCit-BAD

   Plasmid

   #113459

   Purpose

   ProteinA-mCitrine-BAD expression vector (positive control together with pcDNA3.1 NL-BCL2L1)

   Depositor

   Erich Wanker

   Article

   Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

   Insert

   BCL2 associated agonist of cell death (BAD Human)
   

   Tags

   ProteinA-mCitrine

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1_mGFP-FGFR3-K650E

   Plasmid

   #191757

   Purpose

   Expression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-K650E mutation (associated with TD2; target mutation: c.1948A>G; silent mutation added on purpose: c.1938C>T)

   Depositor

   Irene Tiemann-Boege

   Article

   Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi

   Insert

   mGFP-FGFR3IIIc
   

   Tags

   mGFP

   Expression

   Mammalian

   Mutation

   c.1948A>G (p.K650E) + silent mutation c.1938C&…

   Available Since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pY005 (pcDNA3.1-hLb3Cpf1)

   Plasmid

   #69977

   Purpose

   Expresses humanized Lb3Cpf1

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   hLb3Cpf1
   

   Use

   CRISPR

   Tags

   NLS & 3xHA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3.1(-) Hu 4-1BB(CD137)-Fc

   Plasmid

   #183251

   Purpose

   Soluble expression of human 4-1BB (CD137)-Fc. Human IgG1 Fc.

   Depositor

   Alexander McLellan

   Insert

   41BB Fc
   

   Expression

   Mammalian

   Available Since

   Aug. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-aDTX-lumitoxin

   Plasmid

   #51686

   Purpose

   Expression vector for aDTX-lumitoxin

   Depositor

   Edward Boyden

   Article

   Schmidt et al Nat Commun. 2014 Jan 10;5:3019. doi: 10.

   Insert

   aDTX-lumitoxin
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1 myc-PITPalpha

   Plasmid

   #58277

   Purpose

   mammalian expression of human PITP alpha

   Depositor

   Shamshad Cockcroft

   Insert

   PITP alpha (PITPNA Human)
   

   Tags

   myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 21, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3.1-RaACR_687-EYFP

   Plasmid

   #111077

   Purpose

   Anion channelrhodopsin from Rhodomonas abbreviata for expression in mammalian cells

   Depositor

   John Spudich

   Article

   Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

   Insert

   Anion channelrhodopsin RaACR_687
   

   Tags

   EYFP

   Expression

   Mammalian

   Available Since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1(+)-ExRai-AKAR(T/A)

   Plasmid

   #118406

   Purpose

   Negative-control mutant for ExRai-AKAR biosensor.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   ExRai-AKAR(T/A)
   

   Tags

   6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Mutation

   Contains Thr-to-Ala mutation in substrate sequenc…

   Promoter

   CMV

   Available Since

   April 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1/Hygro(-)-B2-TS3

    Plasmid

    #104353

    Purpose

    Expresses human beta 2 integrin tension sensor

    Depositor

    Pontus Nordenfelt, Timothy Springer

    Article

    Nordenfelt et al Nat Commun. 2016 Oct 10;7:13119. doi: 10

    Insert

    Human integrin b2 with tension sensor (ITGB2 Human)
    

    Expression

    Mammalian

    Mutation

    AA1-733 ITGB2 present

    Promoter

    CMV

    Available Since

    Jan. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. Mouse NCKX3 (pcDNA3.1+)

    Plasmid

    #75204

    Purpose

    Mammalian expression of SLC24A3

    Depositor

    Jonathan Lytton

    Article

    Kraev et al J Biol Chem. 2001 Jun 22;276(25):23161-7

    Insert

    SLC24A3 (Slc24a3 Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1_ 2xFLAG-2xSTREP_BACH1_Y11F

    Plasmid

    #159132

    Purpose

    Expresses BACH1 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

    Insert

    BACH1 (BACH1 Human)
    

    Tags

    2X-FLAG _ 2X-STREP

    Expression

    Mammalian

    Mutation

    Y11F

    Promoter

    CMV

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pY117 (pcDNA3.1-huMb3Cpf1)

    Plasmid

    #92293

    Purpose

    Expression of humanized Mb3Cpf1

    Depositor

    Feng Zhang

    Insert

    huMb3Cpf1
    

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Available Since

    June 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1+_LN-HA-AGO2-WT

    Plasmid

    #92009

    Purpose

    Expresses Lambda-N-HA-AGO2 (WT)

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    AGO2 (AGO2 Human)
    

    Tags

    Lambda-N-HA

    Expression

    Mammalian

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1(+)-TTF-1-HDD

    Plasmid

    #52063

    Purpose

    It encodes a mutant of TTF-1 in which the homeodomain is deleted

    Depositor

    David Mu

    Article

    Runkle et al J Biol Chem. 2012 Aug 17;287(34):28790-8

    Insert

    Thyroid Transcription Factor 1 (NKX2-1 Human)
    

    Expression

    Mammalian

    Mutation

    Homeodomain deleted

    Available Since

    April 11, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3.1-CHRFAM7A-mGFP

    Plasmid

    #62633

    Purpose

    mammalian expression of human duplicated Alpha7 (CHRFAM7A) with mEGFP fused into M3-M4 loop

    Depositor

    Henry Lester

    Article

    Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

    Insert

    CHRFAM7A (CHRFAM7A Human)
    

    Tags

    mEGFP inserted in M3-M4 loop

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1(+)_Granulin 2+linker3

    Plasmid

    #176920

    Purpose

    express Granulin 2 with linker 3 in mammalian cells

    Depositor

    Thomas Kukar

    Article

    Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

    Insert

    Granulin 2+linker3 (GRN Human)
    

    Tags

    Twin-Strep and FLAG tags encoded after Signal Pep…

    Expression

    Mammalian

    Available Since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. HA-USPL1-C236A-pcDNA3.1

    Plasmid

    #85764

    Purpose

    mammalian expression of full length HA tagged USPL1, catalytic inactive variant C236A

    Depositor

    Frauke Melchior

    Article

    Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

    Insert

    USPL1, full length, catalytic inactive variant variant C236A (USPL1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    catalytic inactive mutant C236A

    Promoter

    CMV

    Available Since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. HA-USPL1-C236S-pcDNA3.1

    Plasmid

    #85763

    Purpose

    mammalian expression of full length HA tagged USPL1, catalytic inactive variant C236S

    Depositor

    Frauke Melchior

    Article

    Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

    Insert

    USPL1, full length, catalytic inactive variant variant C236S (USPL1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    catalytic inactive mutant C236S

    Promoter

    CMV

    Available Since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1(+)Gαi1-91-NLuc

    Plasmid

    #207148

    Purpose

    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI1 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

    Depositor

    Dominique Schols, Tom Van Loy

    Article

    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

    Insert

    GNAI1 (GNAI1 Human)
    

    Expression

    Mammalian

    Mutation

    NLuc inserted at position L(91)K

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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