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pAc5.1B-lambdaN-HA-DmLSm4_A

Plasmid

#145889

Purpose

Insect Expression of Dm-Lsm4

Depositor

Elisa Izaurralde

Publication

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

Dm-Lsm4

Expression

Insect

Mutation

one non silent mutation G127R compared to the seq…

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCF525-EF1a-Hygro-P2A-AcrIIA4-lenti

Plasmid

#115795

Purpose

Expresses AcrIIA4 in human cells, contained in a lentiviral vector.

Depositor

Jennifer Doudna

Publication

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

AcrIIA4

Use

Lentiviral

Expression

Bacterial and Mammalian

Promoter

EF-1a promoter

Available since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pCDH-CMV-MSI1(FL)

Plasmid

#60358

Purpose

express mouse MSI1 in mammalian cells

Depositor

Sean Ryder

Publication

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (FL) (Msi1 Mouse)

Expression

Bacterial

Promoter

CMV

Available since

Nov. 26, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pVETL-Cmcs-Flag-Ataxin3Q80

Plasmid

#22120

Depositor

Henry Paulson

Publication

Todi et al J Biol Chem. 2007 Oct 5. 282(40):29348-5

Insert

Ataxin 3 (ATXN3 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only
CMV-dH3-YFP

Plasmid

#8694

Depositor

Kami Ahmad

Publication

Janicki et al Cell 2004 Mar 5;116(5):683-98.

Insert

H3 (His3:CG31613 Fly)

Tags

YFP

Expression

Mammalian

Available since

June 30, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pOPINK-OTUD1 (OTU+UIM, aa 287-481)

Plasmid

#61406

Purpose

Expresses human OTUD1 (OTU+UIM domains) in E. coli.

Depositor

David Komander

Publication

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD1 (OTUD1 Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-286.

Promoter

T7

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pIE2-DBD

Plasmid

#21602

Depositor

Takahiro Kusakabe

Publication

Mon et al Anal Biochem. 2009 Sep 15;392(2):180-2.

Insert

GAL4 DBD (GAL4 Budding Yeast)

Use

Gateway Cloning

Tags

3xHA and Gateway destination cassette

Expression

Insect

Available since

March 9, 2010

Availability

Academic Institutions and Nonprofits only

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YFP-ICP0-C3

Plasmid

#134559

Purpose

Expresses YFP-ICP0 fusion protein

Depositor

Susan Janicki

Publication

Newhart et al J Cell Sci. 2012 Nov 15;125(Pt 22):5489-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ICP0

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-C12orf66

Plasmid

#87047

Purpose

mammalian expression of C12orf66

Depositor

David Sabatini

Publication

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

C12orf66 (C12orf66 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5 CASTOR1-HA Cterm

Plasmid

#84487

Purpose

overexpression of CASTOR1 with HA tag

Depositor

David Sabatini

Publication

Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CASTOR1 (GATSL3 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pMT440

Plasmid

#8609

Depositor

Robert Hartley

Publication

Yazynin et al Gene 1996 Feb 22;169(1):131-2.

Insert

barnase, barstar

Tags

as pMT416

Expression

Bacterial

Mutation

V36 codon of barnase of pMT416 replaced by polyli…

Available since

Jan. 17, 2007

Availability

Academic Institutions and Nonprofits only
YFP-Sp100B-C3

Plasmid

#134553

Purpose

Expresses YFP-Sp100B fuson protein

Depositor

Susan Janicki

Publication

Newhart et al Mol Biol Cell. 2013 Mar 13.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Sp100B

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pHIS3p:CloverGFP-Tub1+3'UTR::HIS3

Plasmid

#50654

Depositor

Wei-Lih Lee

Publication

Markus et al Traffic. 2015 Jul;16(7):773-86. doi: 10.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CloverGFP-Tub1

Use

Yeast expression

Tags

CloverGFP

Promoter

HIS3p

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET-28a mScarlet

Plasmid

#178543

Purpose

The mScarlet fluorescent protein with an N-terminal 6xHis-tag in pET28a.

Depositor

Siegfried Musser

Publication

Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

Insert

mScarlet

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pNC1136

Plasmid

#41562

Depositor

Beverly Errede

Publication

Houser et al Yeast. 2012 Dec;29(12):519-30. doi: 10.1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Ura3-FUS1-UBIYdkGFP*-SpHis5-Tim9

Mutation

GFP mutations: Phe 64 to Leu, S65 to Thr, V163 t…

Available since

April 2, 2013

Availability

Academic Institutions and Nonprofits only

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pVETL-Cmcs-Flag-Ataxin3Q25

Plasmid

#22118

Depositor

Henry Paulson

Publication

Todi et al J Biol Chem. 2007 Oct 5. 282(40):29348-5

Insert

Ataxin 3 (ATXN3 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only
HA Raptor mutant 4

Plasmid

#8474

Depositor

David Sabatini

Publication

Kim et al Cell 2002 Jul 26;110(2):163-75.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Raptor (RPTOR Human)

Tags

ha

Expression

Mammalian

Mutation

391SQ392-PA (S391P, Q392A)

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
HA Raptor mutant 1

Plasmid

#8470

Depositor

David Sabatini

Publication

Kim et al Cell 2002 Jul 26;110(2):163-75.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Raptor (RPTOR Human)

Tags

ha

Expression

Mammalian

Mutation

194YDC196-AAA (Y194A, D195A, C196A)

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
deltaCT S6K1 MSCV puro

Plasmid

#8465

Depositor

David Sabatini

Publication

Ali et al J Biol Chem 2005 Apr 4;.

Insert

delta ct p70 S6K1 (Rps6kb1 Rat)

Use

Retroviral

Tags

myc

Expression

Mammalian

Mutation

deletion of 104 c-terminal amino acids

Available since

May 11, 2005

Availability

Academic Institutions and Nonprofits only
pKEW212-MBP-TEV-AcrVA4

Plasmid

#115658

Purpose

Expresses AcrVA4 in bacteria via T7 RNAP, for purification

Depositor

Jennifer Doudna

Publication

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

AcrVA4

Tags

MBP-TEV

Expression

Bacterial

Promoter

T7 RNAP

Available since

Oct. 5, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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