We narrowed to 7,895 results for: sms

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pRK5-FLAG-C12orf66

Plasmid

#87047

Purpose

mammalian expression of C12orf66

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

C12orf66 (C12orf66 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5 CASTOR1-HA Cterm

Plasmid

#84487

Purpose

overexpression of CASTOR1 with HA tag

Depositor

David Sabatini

Article

Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

Insert

CASTOR1 (GATSL3 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only
pMT440

Plasmid

#8609

Depositor

Robert Hartley

Article

Yazynin et al Gene 1996 Feb 22;169(1):131-2.

Insert

barnase, barstar

Tags

as pMT416

Expression

Bacterial

Mutation

V36 codon of barnase of pMT416 replaced by polyli…

Available Since

Jan. 17, 2007

Availability

Academic Institutions and Nonprofits only
pHIS3p:CloverGFP-Tub1+3'UTR::HIS3

Plasmid

#50654

Depositor

Wei-Lih Lee

Article

Markus et al Traffic. 2015 Jul;16(7):773-86. doi: 10.

Insert

CloverGFP-Tub1

Use

Yeast expression

Tags

CloverGFP

Promoter

HIS3p

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pET-28a mScarlet

Plasmid

#178543

Purpose

The mScarlet fluorescent protein with an N-terminal 6xHis-tag in pET28a.

Depositor

Siegfried Musser

Article

Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

Insert

mScarlet

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pScaf-7560.1

Plasmid

#111406

Purpose

pScaf phagemid with insert for generating DNA origami scaffold 7560 bases in length

Depositor

Shawn Douglas

Article

Nafisi et al Synth Biol (Oxf). 2018 Jan;3(1). doi: 10

Insert

pScaf-7560.1

Expression

Bacterial

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pNC1136

Plasmid

#41562

Depositor

Beverly Errede

Article

Houser et al Yeast. 2012 Dec;29(12):519-30. doi: 10.1

Insert

Ura3-FUS1-UBIYdkGFP*-SpHis5-Tim9

Mutation

GFP mutations: Phe 64 to Leu, S65 to Thr, V163 t…

Available Since

April 2, 2013

Availability

Academic Institutions and Nonprofits only

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pVETL-Cmcs-Flag-Ataxin3Q80

Plasmid

#22120

Depositor

Henry Paulson

Article

Todi et al J Biol Chem. 2007 Oct 5. 282(40):29348-5

Insert

Ataxin 3 (ATXN3 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only
HA Raptor mutant 4

Plasmid

#8474

Depositor

David Sabatini

Article

Kim et al Cell 2002 Jul 26;110(2):163-75.

Insert

Raptor (RPTOR Human)

Tags

ha

Expression

Mammalian

Mutation

391SQ392-PA (S391P, Q392A)

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
HA Raptor mutant 1

Plasmid

#8470

Depositor

David Sabatini

Article

Kim et al Cell 2002 Jul 26;110(2):163-75.

Insert

Raptor (RPTOR Human)

Tags

ha

Expression

Mammalian

Mutation

194YDC196-AAA (Y194A, D195A, C196A)

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
deltaCT S6K1 MSCV puro

Plasmid

#8465

Depositor

David Sabatini

Article

Ali et al J Biol Chem 2005 Apr 4;.

Insert

delta ct p70 S6K1 (Rps6kb1 Rat)

Use

Retroviral

Tags

myc

Expression

Mammalian

Mutation

deletion of 104 c-terminal amino acids

Available Since

May 11, 2005

Availability

Academic Institutions and Nonprofits only
pOPINK-OTUD6B (full-length, aa 1-293)

Plasmid

#61418

Purpose

Expresses human OTUD6B (full-length) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD6B (OTUD6B Human)

Tags

His6-GST-3C

Expression

Bacterial

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pKEW212-MBP-TEV-AcrVA4

Plasmid

#115658

Purpose

Expresses AcrVA4 in bacteria via T7 RNAP, for purification

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

AcrVA4

Tags

MBP-TEV

Expression

Bacterial

Promoter

T7 RNAP

Available Since

Oct. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pTU2-A-RFP (p15A origin)

Plasmid

#74090

Purpose

pTU2-A backbone, with RFP negative selection and p15A (low-copy) origin of replication

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Golden Gate destination vector - Level 2, 4 TU's with RFP and p15A origin

Use

Synthetic Biology

Expression

Bacterial

Promoter

R0010 Const. Promoter controlling RFP

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-C12orf66

Plasmid

#87048

Purpose

mammalian expression of C12orf66

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

C12orf66 (C12orf66 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-ITFG2

Plasmid

#87044

Purpose

mammalian expression of ITFG2

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

ITFG2 (ITFG2 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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Mis12-targted Aurora B FRET sensor

Plasmid

#45231

Depositor

Michael Lampson

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

Mis12 targeted Aurora B FRET sensor

Expression

Mammalian

Available Since

May 31, 2013

Availability

Academic Institutions and Nonprofits only

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pOPINK-OTUD3 (OTU+UBA, aa 52-275)

Plasmid

#61411

Purpose

Expresses human OTUD3 (OTU+UBA domains) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD3 (OTUD3 Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-51 and aa 276-398.

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 TaGu N-1F

Plasmid

#203762

Purpose

For expression of N-flag tagged TaGu R2 protein in mammalian cells

Depositor

Kathleen Collins

Article

Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

Insert

R2 retroelement protein

Tags

Flag

Expression

Mammalian

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-UBE2S-UBD

Plasmid

#66713

Purpose

Expresses the UBE2S-UBD fusion protein in E. coli

Depositor

David Komander

Article

Bremm et al Nat Struct Mol Biol. 2010 Aug;17(8):939-

Inserts

e2-epf (UBE2S )
isot (USP5 )

Tags

GST-3C

Expression

Bacterial

Promoter

T7

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only