We narrowed to 17,211 results for: EMB

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pcU6_3 sgRNA

Plasmid

#92359

Purpose

Chicken-specific U6 sgRNA expression mini-vector, harbouring chick U6_3 pol III promoter.

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

chick U6.3 promoter and gRNA cloning cassette

Use

CRISPR

Expression

Mammalian

Promoter

chick U6.3

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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SIN40C.SFFV.dCas9-KRAB.P2A.mNeon

Plasmid

#170482

Purpose

Lentiviral dSpCas9-KRAB (CRISPRi) delivery, mNeon coexpression

Depositor

Dirk Heckl

Article

Ng et al iScience. 2023 Sep 7;26(10):107844. doi:

Inserts

dCas9-KRAB
SFFV
P2A-mNeonGreen

Use

Lentiviral

Tags

HA

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pDM1383

Plasmid

#207423

Purpose

Extrachromosomal expression HSPC300-GFP and mCherry-Hs Raf1 (1–134 RBD)

Depositor

Rob Kay

Article

Veltman et al Elife. 2016 Dec 13;5:e20085. doi: 10.755

Inserts

HSPC300
Raf1 (RAF1 Human)

Use

Unspecified

Tags

GFP and mCherry

Mutation

aa 1–134 (RBD) only

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pWZL-hygro-FAKWT

Plasmid

#216540

Purpose

This retroviral plasmid expresses the human wild type PTK2

Depositor

Georgia Konstantinidou

Article

Pozzato et al EMBO Mol Med. 2024 Sep 13. doi: 10.1038/

Insert

PTK2 (PTK2 Human)

Use

Retroviral

Tags

No

Expression

Mammalian

Mutation

wild type sequence

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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Neurl1-Flag pcDNA3.1

Plasmid

#17282

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

Insert

Neuralized-like (Neurl1a Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
pAS_8xMmaPylT_EF1_FLAG-MmaPylRS

Plasmid

#174890

Purpose

Mma PylRS expression for amber suppression in mammalian cells; transient or piggy bac mediated integration

Depositor

Simon Elsaesser

Article

van Husen et al Chembiochem. 2021 Aug 25. doi: 10.1002/c

Insert

Mma PylRS (pylS Methanosarcina mazei)

Tags

FLAG

Expression

Mammalian

Mutation

U25C PylT

Promoter

EF1

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-Tau-mCherry-RING

Plasmid

#233689

Purpose

Inducible expression of Tau-mCherry-RING

Depositor

Leo James

Article

Miller et al Cell. 2024 Oct 17;187(21):5967-5980.e17.

Insert

Tau-mCherry-RING

Use

Lentiviral

Expression

Mammalian

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-mOC-STAMP-GFP

Plasmid

#73579

Purpose

Lentivirus expression of mOC-STAMP

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 Jun 4;10(6):e0128275. doi

Insert

OC-STAMP (Ocstamp Mouse)

Use

Lentiviral

Tags

GFP

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
pAG496

Plasmid

#226750

Purpose

Expresses exogenous MTCH2

Depositor

Jonathan Weissman

Article

Guna et al Science. 2022 Oct 21;378(6617):317-322.

Insert

MTCH2 (MTCH2 Human)

Use

Lentiviral

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-PTEN

Plasmid

#116866

Purpose

Recruitable PTEN dual-specificity phosphatase

Depositor

Gerry Hammond

Article

Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

Insert

PTEN (PTEN Human)

Tags

FKBP12 and mCherry

Expression

Mammalian

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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eSpCas9(1.1)-Puro

Plasmid

#117686

Purpose

The plasmids pSpCas9 (BB)-2A-Puro (Addgene, #48139) and eSpCas9 (1.1) (Addgene, #71814) were combined to express both “enhanced specificity” Cas9 and puromycin resistance protein.

Depositor

Luc Leyns

Article

Liu et al Biochem Biophys Res Commun. 2019 Feb 14.

Insert

eSpCas9(1.1)

Use

CRISPR

Tags

PuroR (R166H)

Expression

Mammalian

Mutation

K848A, K1003A, R1060A

Promoter

U6

Available Since

Feb. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA c-Jun

Plasmid

#237333

Purpose

transient overexpression in mammalian cells

Depositor

Michele Pagano

Article

Rizzini et al Curr Biol. 2019 Jun 17;29(12):1954-1962.

Insert

c-Jun (JUN Human)

Tags

HA

Expression

Mammalian

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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PAmCherry-N1-FRB-Tubbyc

Plasmid

#202678

Purpose

Photoactivatable and rapamycin recruitable lipid biosensor

Depositor

Gerry Hammond

Article

Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

Insert

Mus musculus Tub (243-505):MTOR(2021-2113):PAmCherry1 (Tub, Mtor Mouse)

Expression

Mammalian

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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pEYFP-hDF

Plasmid

#72584

Purpose

human dysferlin full length tagged with YFP

Depositor

Renzhi Han

Article

Xu et al PLoS One. 2011;6(11):e27884. doi: 10.137

Insert

dysferlin (DYSF Human)

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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mouse Drp1(K38A)

Plasmid

#26049

Depositor

David Chan

Article

Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

Insert

Drp1 (Dnm1l Mouse)

Expression

Mammalian

Mutation

K38A GTPase mutant

Available Since

Sept. 20, 2010

Availability

Academic Institutions and Nonprofits only
pHR-CMV-TetO2_HA-BirA

Plasmid

#113896

Purpose

Mammalian (inducible) expression of HA-tagged cytosolic E. coli biotin ligase

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Insert

E. coli biotin ligase

Use

Lentiviral

Tags

HA

Promoter

CMV-MIE-TetO2

Available Since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pLV hUbC-dSaCas9-KRAB-T2A-PuroR

Plasmid

#106249

Purpose

Lentiviral vector with puro selection for expression of S. aureus dCas9-KRAB

Depositor

Charles Gersbach

Article

Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

Insert

dead S. aureus Cas9 KRAB T2A PuroR

Use

CRISPR and Lentiviral

Tags

HA-NLS and NLS-KRAB

Expression

Mammalian

Mutation

D10A and N580A

Promoter

hUbC

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCMMP-BiP-IRES-mRFP

Plasmid

#36975

Depositor

Bill Sugden

Article

Pratt et al J Virol. 2012 Apr;86(8):4380-93. Epub 20

Insert

BiP (HSPA5 Human)

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

May 16, 2012

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-HsGIGYF1_AI

Plasmid

#148961

Purpose

Mammalian Expression of HsGIGYF1

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2019 Oct 1;33(19-20):1355-136

Insert

HsGIGYF1 (GIGYF1 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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CLC-EGFP

Plasmid

#193566

Purpose

Expresses human Clathrin Light Chain B tagged with mEGFP in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain B (CLTB Human)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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