We narrowed to 14,476 results for: NTS

1. pCC_08 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dxCas9NG-NLS-VPR-2A-Puro-WPRE

   Plasmid

   #139093

   Purpose

   Expresses human codon-optimized inactive xCas9-NG fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

   Depositor

   Neville Sanjana

   Article

   Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

   Insert

   dxCas9-NG-VPR
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A, H840A, A262T,R324L, S409I, E480K, E543D, M6…

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHEE401E

   Plasmid

   #71287

   Purpose

   Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance

   Depositor

   Qi-Jun Chen

   Article

   Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

   Inserts

   gRNA scaffold
   zCas9
   

   Use

   CRISPR; Plant binary vector

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Zea mays codon-optimized Cas9

   Promoter

   EC1.2 enhancer fused to EC1.1 promoter and U6-26p…

   Available Since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. PCR4-Shh::lacZ-H11

   Plasmid

   #139098

   Purpose

   enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Shh minimal promoter)

   Depositor

   Len Pennacchio

   Article

   Kvon et al Cell. 2020 Mar 6. pii: S0092-8674(20)302

   Type

   Empty backbone

   Use

   CRISPR and Mouse Targeting

   Expression

   Mammalian

   Promoter

   Shh

   Available Since

   March 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDY0209 ACTB Nicking guide +48

   Plasmid

   #179109

   Purpose

   ACTB N-term Nicking guide 1 +48 guide

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

   Insert

   ACTB N-term Nicking guide 1 +48 guide
   

   Use

   CRISPR

   Available Since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR223_TNFRSF17_WT

   Plasmid

   #82263

   Purpose

   Gateway Donor vector containing TNFRSF17 , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, William Hahn, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   TNFRSF17 (TNFRSF17 Human)
   

   Use

   Gateway entry vector

   Mutation

   N81S; K86E

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGP-CMV-jGCaMP7s

   Plasmid

   #104463

   Purpose

   Mammalian expression of ultrasensitive protein calcium sensor

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Insert

   jGCaMP7s
   

   Tags

   T7 epitope, Xpress tag, 6xHis

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR223_IRGM_WT

   Plasmid

   #82134

   Purpose

   Gateway Donor vector containing IRGM , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, William Hahn, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   IRGM (IRGM Human)
   

   Use

   Gateway entry vector

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV_msfGFP-SEPT7

   Plasmid

   #180315

   Purpose

   mammalian expression of human SEPT7 fused to monomeric superfolder GFP

   Depositor

   Manos Mavrakis

   Article

   Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

   Insert

   SEPTIN7 (SEPTIN7 Human)
   

   Tags

   msfGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR223_NOTCH2_WT

   Plasmid

   #82292

   Purpose

   Gateway Donor vector containing NOTCH2, part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, William Hahn, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   NOTCH2 (NOTCH2 Human)
   

   Use

   Gateway entry vector

   Mutation

   C946Y

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC19-U6-BsmBI_epegRNA_entry-mpknot_(LM1140)

    Plasmid

    #228253

    Purpose

    pU6 entry plamid for cloning prime editor mpknot epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    mpknot entry plasmid backbone for epegRNA expression via human U6 promoter
    

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    U6

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. HDM_SARS2_Spike_del21_D614G

    Plasmid

    #158762

    Purpose

    Mammalian expression vector for expressing SARS-2 Spike with 21 aa del at C-terminus and pt mutation D to G at site 614

    Depositor

    Jesse Bloom

    Article

    Crawford et al Viruses. 2020 May 6;12(5). pii: v1205051

    Insert

    SARS2-Spike_del21_D614G (S Synthetic, Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Mammalian

    Mutation

    deleted amino acids 1257-1278, changed aspartic a…

    Promoter

    CMV

    Available Since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR223_RBM10_WT

    Plasmid

    #81958

    Purpose

    Gateway Donor vector containing RBM10, part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    RBM10 (RBM10 Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDH-SFFV-GLuc-CreERT2-mCherry

    Plasmid

    #178185

    Purpose

    the plasmid promotes the constitutive expression of CreERT2 to enable intracellular recombination, Gaussia Luc can be used for in vivo imaging and mCherry is used as a marker for ex vivo analyses.

    Depositor

    Irmela Jeremias

    Article

    Carlet et al Nat Commun. 2021 Sep 27;12(1):5655. doi:

    Inserts

    GLuc
    CreERT2
    mCherry
    

    Use

    Lentiviral

    Available Since

    Jan. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. PCR4-Hsp68::lacZ-H11

    Plasmid

    #139099

    Purpose

    enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Hsp68 minimal promoter)

    Depositor

    Len Pennacchio

    Article

    Kvon et al Cell. 2020 Mar 6. pii: S0092-8674(20)302

    Type

    Empty backbone

    Use

    CRISPR and Mouse Targeting

    Expression

    Mammalian

    Promoter

    Hsp68

    Available Since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR223_PIK3CB_WT

    Plasmid

    #82221

    Purpose

    Gateway Donor vector containing PIK3CB , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    PIK3CB (PIK3CB Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDY0216 PASTEv1 PE2-P2A-Bxb1

    Plasmid

    #179104

    Purpose

    PASTEv1 PE2-P2A-Bxb1

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    pCMV-PE2-P2A-Bxb1
    

    Expression

    Mammalian

    Available Since

    Feb. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR223_SUV39H1_WT

    Plasmid

    #82236

    Purpose

    Gateway Donor vector containing SUV39H1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    SUV39H1 (SUV39H1 Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAR014 Lenti pEF-dEnAsCas12a-NLS-VPR-3XHA-T2A-BSD

    Plasmid

    #195548

    Purpose

    dCas12a effector fusion for manipulating transcription

    Depositor

    Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Lenti pEF-dEnAsCas12a-NLS-VPR-3XHA-T2A-BSD
    

    Use

    CRISPR and Lentiviral

    Tags

    3XHA

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLentiCMV Puro DEST p38KTRmCerulean3

    Plasmid

    #59155

    Purpose

    Lentiviral vector to express p38 KTR mCerulean3 under CMV promoter (With Puromycin Resistance)

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    p38 Kinase Translocation Reporter (MAPK14 Mouse, Human)
    

    Use

    Lentiviral

    Tags

    mCerulean3

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. kiCAP-AAV-MyoAAV3F

    Plasmid

    #224447

    Purpose

    Rep/Cap plasmid for the production of MyoAAV 3F, a muscle-tropic AAV capsid in mice and macaques.

    Depositor

    Pardis Sabeti

    Article

    Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

    Insert

    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    

    Use

    AAV

    Mutation

    RGDHASW insert between amino acids 588 and 589 of…

    Promoter

    p41

    Available Since

    Sept. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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