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pETDUET-GST AO7REwt

Plasmid

#213935

Purpose

GST tagged AO7 encoding aa 126-258 for expression in bacteria

Depositor

Allan Weissman

Article

Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

Insert

AO7 aa 126-258 (RNF25 Human)

Tags

GST

Expression

Bacterial

Mutation

AO7 cloned into the first multiple cloning site o…

Promoter

T7 promoter-1

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pCC_01 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9-NLS-2A-Puro-WPRE

Plasmid

#139086

Purpose

Expresses human codon-optimized SpCas9 nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

Depositor

Neville Sanjana

Article

Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

Insert

SpCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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AA331

Plasmid

#215954

Purpose

Fragmid fragment: (guide cassette) guide expression

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

U6_v1; BsmBI_v0; BsmBI_v1; trRNA_v2 [Sa]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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p5E-1.7kbfabp6

Plasmid

#159087

Purpose

p5E with promoter sequence 1.7kb upstream of zebrafish fabp6 gene

Depositor

John Rawls

Article

Wen et al Sci Adv. 2021 Jul 23;7(30). pii: 7/30/ea

Insert

1.7kb fabp6 promoter (fabp6 Zebrafish)

Use

Promoter fragment 5' entry vector for tol2 c…

Promoter

1.7kb fabp6

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pEntry_MERS-CoV-nsp3

Plasmid

#168838

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

MERS-CoV-nsp3 (orf1ab )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pLCHKOv3-mCherry

Plasmid

#209035

Purpose

Lentiviral backbone for expressing U6 driven hybrid guide (hg)RNAs with BveI cloning sites, mCherry and puromycin selection marker

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDipA-TEV-His12

Plasmid

#137042

Purpose

expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi DipA with C-terminal TEV-protease-cleavable His12

Depositor

Debra Hansen

Article

Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

Insert

AAC66790.1 (BB_0418 Borrelia burgdorferi B31)

Tags

His12 (TEV protease cleavable)

Expression

Bacterial

Mutation

wild type, full-length protein, contains signal p…

Promoter

T7

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO HIS HSPH2

Plasmid

#19535

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

heat shock 70kDa protein 4 (HSPA4 Human)

Tags

His

Expression

Mammalian

Mutation

mutations A381T, T732A, and an insertion of "…

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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FAK2_HUMAN_D0

Plasmid

#79747

Purpose

This plasmid encodes the kinase domain of FAK2. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

FAK2 (PTK2B Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pCCLc-MND-B2705-KK10-SABR

Plasmid

#119053

Purpose

3rd gen transfer vector. HLA-B2705 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains the HIV(gag) KK10 epitope "KRWIILGLNK" cloned via BsmBI

Depositor

David Baltimore

Article

Joglekar et al Nat Methods. 2019 Feb;16(2):191-198. doi

Insert

B2705-KK10-SABR

Use

Lentiviral

Expression

Mammalian

Promoter

MND

Available Since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pEntry_MERS-CoV-Nucleocapsid

Plasmid

#168833

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

MERS-CoV-Nucleocapsid (N )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV/myc/ER-αT9ib

Plasmid

#110768

Purpose

Expresses ER intrabody against mouse and human TLR9

Depositor

Thomas Böldicke

Article

Reimer et al Cell Mol Biol Lett. 2013 Sep;18(3):433-4

Insert

intrabody against mouse and human TLR9

Tags

ER retention signal (SEKDEL), ER signal peptide, …

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO HSPH2

Plasmid

#19454

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

heat shock 70kDa protein 4 (HSPA4 Human)

Expression

Mammalian

Mutation

mutations A381T, T732A, and an insertion of "…

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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AA008

Plasmid

#216003

Purpose

Fragmid fragment: (Cas protein) preferred over wildtype Cas12a

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Cas12a_v1.1 [EnAs]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMEL16

Plasmid

#107922

Purpose

HIS3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Article

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y

Use

CRISPR

Expression

Yeast

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pROS11

Plasmid

#107925

Purpose

amdS based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Article

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y, gRNA-ADE2.Y

Use

CRISPR

Expression

Yeast

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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p221z-erVenusYFP

Plasmid

#71265

Purpose

Entry clone containing ER-localized Venus. Can be used to construct transcriptional reporters. For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Article

Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

Insert

VenusYFP

Use

Gateway

Tags

ER retention signal HDEL and signal peptide (ER)…

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pEntry_SARS-CoV-1-Nucleocapsid

Plasmid

#168852

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

SARS-CoV-1-Nucleocapsid (N )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-OC43-nsp13

Plasmid

#168950

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-OC43-nsp13 (ORF1ab )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pUCIDT-attL1-Human ABeta-attR5

Plasmid

#160436

Purpose

Entry vector for cloning human Amyloid Beta(1-42) using 2-fragment gateway recombination

Depositor

Nicholas Tolwinski

Article

Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi

Insert

Homo sapiens amyloid beta precursor protein (APP Human)

Expression

Bacterial

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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