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We narrowed to 62,929 results for: cloning

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Showing: 9601 - 9620 of 62929 results

  1. POLG-pcDNA3.1

    Plasmid
    #191487
    Purpose
    Expresses human POLG gene on pcDNA3.1 backbone. POLG gene is cloned with BamHI and XbaI restriction sites.
    Depositor
    Hans Zempel
    Insert
    POLG (POLG Human)
    Tags
    V5 tag, 6X His
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    191487-plasmid-map-sequence-id-415364

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  2. pY036_ATP1A1_G3_Array

    Plasmid
    #86619
    Purpose
    Vector for expression of a CRISPR/AsCpf1 array containing the ATP1A1 G3 guide in combination with a user-specified guide. Cloning of oligos for the second guide using BbsI sites. PY036-like plasmid
    Depositor
    Yannick Doyon
    Publication
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G3 crRNA + user-specified crRNA + AsCpf1-3xHA
    Use
    CRISPR; Co-selection via nhej using ouabain
    Tags
    3xHA
    Expression
    Mammalian
    Promoter
    CBh
    Available since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    86619-plasmid-map-sequence-id-168855

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  19. pRDA52_Lenti.puro.U6_modified DR

    Plasmid
    #196723
    Purpose
    For cloning pairs of AsCas12a guides with two DRs that are as different as possible.
    Depositor
    Bernhard Schmierer
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196723-plasmid-map-sequence-id-389696

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  20. pLgw ELK1Y158A-V5-EcoDam

    Plasmid
    #98609
    Purpose
    Mammalian DamID lentiviral vector for fusing ELK1Y158A with Dam-V5 using Gateway cloning
    Depositor
    Richard Harvey
    Insert
    ELK1Y158A (Elk1 Mouse)
    Use
    Lentiviral; Damid
    Tags
    V5 and Dam (DNA adenine methyltransferase)
    Expression
    Mammalian
    Promoter
    Heat Shock Minimal Promoter
    Available since
    July 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    98609-plasmid-map-sequence-id-189409

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Showing: 9601 - 9620 of 62929 results