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pGEMHE:mTREK-1(S131A)

Plasmid

#224745

Purpose

In vitro transcription for Oocyte expression of mTREK-1(S131A)

Depositor

Dan Minor

Publication

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Mutation

S131A

Promoter

T7

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1(G171F)

Plasmid

#224747

Purpose

In vitro transcription for Oocyte expression of mTREK-1(G171F)

Depositor

Dan Minor

Publication

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Mutation

G171F

Promoter

T7

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1(A286F)

Plasmid

#224746

Purpose

In vitro transcription for Oocyte expression of mTREK-1(A286F)

Depositor

Dan Minor

Publication

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Mutation

A286F

Promoter

T7

Available since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pKL1-100

Plasmid

#219408

Purpose

Reporter gene (4 operators) for zinc finger activator

Depositor

Ahmad Khalil

Publication

Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.

Insert

(42-10 op)x4-minCyc1-Venus-tCyc1

Expression

Yeast

Available since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

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pKL1-422

Plasmid

#219410

Purpose

Reporter gene (1 operator) for zinc finger activator

Depositor

Ahmad Khalil

Publication

Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.

Insert

(42-10 op)x1-minCyc1-Venus-tCyc1

Expression

Yeast

Available since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

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pKL1-424

Plasmid

#219412

Purpose

Reporter gene (3 operator) for zinc finger activator

Depositor

Ahmad Khalil

Publication

Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.

Insert

(42-10 op)x3-minCyc1-Venus-tCyc1

Expression

Yeast

Available since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

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pKL1-095

Plasmid

#219407

Purpose

Reporter gene (4 operators) for zinc finger activator

Depositor

Ahmad Khalil

Publication

Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.

Insert

(13-6 op)x4-minCyc1-Venus-tCyc1

Expression

Yeast

Available since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

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pKL1-423

Plasmid

#219411

Purpose

Reporter gene (2 operator) for zinc finger activator

Depositor

Ahmad Khalil

Publication

Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.

Insert

(42-10 op)x2-minCyc1-Venus-tCyc1

Expression

Yeast

Available since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

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TRAPa(R150A)-3xFLAG-pCDNA

Plasmid

#201966

Purpose

Mammalian expression of mutated Translocon-associated protein subunit alpha

Depositor

Ville Paavilainen

Publication

Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

Insert

SSR1 (SSR1 Human)

Tags

3xFLAG-tag

Expression

Mammalian

Mutation

R150A

Available since

Jan. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pMx/ATP10A(E203Q)-HA

Plasmid

#209258

Purpose

Mammalian expression of ATP10A mutant E203Q

Depositor

Hye-Won Shin

Publication

Naito et al J Biol Chem. 2015 Jun 12;290(24):15004-1

Insert

ATP10A (ATP10A Human)

Use

Retroviral

Tags

HA

Mutation

E203Q

Available since

Nov. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pHR- flag-Lag43-iCAM-tether

Plasmid

#205211

Purpose

This vector encodes of the synCAM tether (iCAM1) and GFP nanobody LAG43

Depositor

Wendell Lim

Publication

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM iCAM1 tether and Lag43 anti-GFP nanobody

Use

Lentiviral

Expression

Mammalian

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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PH-PLCd1-mNGx2

Plasmid

#202752

Purpose

expresses fluorescent protein-tagged lipid biosensor

Depositor

Gerry Hammond

Publication

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

PLCD1v2(1-170): mNG2:mNG2 (PLCD1 Human)

Expression

Mammalian

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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sgRNA1 _8xCTS-ECFP-pA

Plasmid

#200243

Purpose

Mammalian transfections; 8xCTS-ECFP reporter construct 1

Depositor

Tatjana Sauka-Spengler

Publication

Pelea et al bioRxiv 2023.05.08.539738

Insert

sgRNA1_8xCTS

Expression

Mammalian

Available since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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sgRNA1 _1xCTS-ECFP-pA

Plasmid

#200237

Purpose

Mammalian transfections; 1xCTS-ECFP reporter construct 1

Depositor

Tatjana Sauka-Spengler

Publication

Pelea et al bioRxiv 2023.05.08.539738

Insert

sgRNA1_1xCTS

Expression

Mammalian

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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sgRNA5 _1xCTS-ECFP-pA

Plasmid

#200241

Purpose

Mammalian transfections; 1xCTS-ECFP reporter construct 5

Depositor

Tatjana Sauka-Spengler

Publication

Pelea et al bioRxiv 2023.05.08.539738

Insert

sgRNA5_1xCTS

Expression

Mammalian

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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sgRNA3 _1xCTS-ECFP-pA

Plasmid

#200239

Purpose

Mammalian transfections; 1xCTS-ECFP reporter construct 3

Depositor

Tatjana Sauka-Spengler

Publication

Pelea et al bioRxiv 2023.05.08.539738

Insert

sgRNA3_1xCTS

Expression

Mammalian

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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gRNA4 _8xCTS-ECFP-pA

Plasmid

#200246

Purpose

Mammalian transfections; 8xCTS-ECFP reporter construct 4

Depositor

Tatjana Sauka-Spengler

Publication

Pelea et al bioRxiv 2023.05.08.539738

Insert

sgRNA4_8xCTS

Expression

Mammalian

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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sgRNA4 _1xCTS-ECFP-pA

Plasmid

#200240

Purpose

Mammalian transfections; 1xCTS-ECFP reporter construct 4

Depositor

Tatjana Sauka-Spengler

Publication

Pelea et al bioRxiv 2023.05.08.539738

Insert

sgRNA4_1xCTS

Expression

Mammalian

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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sgRNA2 _1xCTS-ECFP-pA

Plasmid

#200238

Purpose

Mammalian transfections; 1xCTS-ECFP reporter construct 2

Depositor

Tatjana Sauka-Spengler

Publication

Pelea et al bioRxiv 2023.05.08.539738

Insert

sgRNA2_1xCTS

Expression

Mammalian

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pJL1-sfGFP-MOORmut

Plasmid

#198213

Purpose

pJL1 plasmid encoding superfolder GFP modified at the C-terminus with 32 amino acids containing a non-permissible minimum optimal O-linked recognition site (MOORmut) followed by a 6x-His tag

Depositor

Michael Jewett

Publication

Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

Insert

sfGFP_MOORmut

Tags

6x His Tag and mutated (non-permissible) MOORmut …

Expression

Bacterial

Promoter

T7

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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