Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 9,739 results for: CAG

Showing: 9621 - 9640 of 9739 results

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pKLV2-U6gRNA5(CXCR4-E3(45))-PGKpuro2ABFP-W

Plasmid

#200482

Purpose

Lentiviral vector expressing gRNA targeting human CXCR4-E3

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

CXCR4-E3(45) (CXCR4 Human)

Use

Lentiviral

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(CXCR4-E4(59))-PGKpuro2ABFP-W

Plasmid

#200485

Purpose

Lentiviral vector expressing gRNA targeting human CXCR4-E4

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

CXCR4-E4(59) (CXCR4 Human)

Use

Lentiviral

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6A-M2WA-F1359A-siRNAres_S

Plasmid

#147518

Purpose

Mammalian Expression of HsTNRC6A-M2WA-F1359A-siRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

HsTNRC6A-M2WA-F1359A-siRNAres (TNRC6A Human)

Expression

Mammalian

Mutation

one non silent mutation K1578E compared to the se…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-HsTNRC6A_1209-1709-CIM1-2mut-F1359A_S

Plasmid

#147522

Purpose

Bacterial Expression of HsTNRC6A_1209-1709-CIM1-2mut-F1359A

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

HsTNRC6A_1209-1709-CIM1-2mut-F1359A (TNRC6A Human)

Expression

Bacterial

Mutation

one non silent mutation K1578E compared to the se…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-HsTNRC6A_1209-1709-M2WA-CIM1-2mut_S

Plasmid

#147527

Purpose

Bacterial Expression of HsTNRC6A_1209-1709-M2W1-CIM1-2mut

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

HsTNRC6A_1209-1709-M2W1-CIM1-2mut (TNRC6A Human)

Expression

Bacterial

Mutation

one non silent mutation K1578E compared to the se…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6A-CIM1-2mut-siRNAres_R

Plasmid

#147412

Purpose

Insect Expression of HsTNRC6A-CIM1-2mut-siRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

HsTNRC6A-CIM1-2mut-siRNAres (TNRC6A Human)

Expression

Insect

Mutation

one non silent mutation K1578E compared to the se…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6A-del1352-1369-siRNAres_K

Plasmid

#146743

Purpose

Mammalian Expression of HsTNRC6A-del1352-1369-siRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

HsTNRC6A-del1352-1369-siRNAres (TNRC6A Human)

Expression

Mammalian

Mutation

one non silent mutation K1578E compared to the se…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-CASK [K56A/57R]

Plasmid

#188217

Purpose

Mammalian Expression Plasmid of anti-CASK (Human). Derived from hybridoma K56A/57

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-CASK (Homo sapiens) recombinant mouse monoclonal antibody (CASK Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-CASK [K56A/57]

Plasmid

#190283

Purpose

Mammalian Expression Plasmid of anti-CASK (Human). Derived from hybridoma K56A/57.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-CASK (Homo sapiens) recombinant Mouse monoclonal antibody (CASK Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6A_1242-1709-F1359A_K

Plasmid

#146742

Purpose

Mammalian Expression of HsTNRC6A_1242-1709-F1359A

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

HsTNRC6A_1242-1709-F1359A (TNRC6A Human)

Expression

Mammalian

Mutation

five non silent mutations D1289G, G1515A, S1516G,…

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6A-delMI-MII_F

Plasmid

#146298

Purpose

Mammalian Expression of HsTNRC6A-delMI-MII

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsTNRC6A-delMI-MII (TNRC6A Human)

Expression

Mammalian

Mutation

Five non silent mutations A339T, V417M, R934G, D1…

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6A-M2WA-siRNAres_S

Plasmid

#147519

Purpose

Mammalian Expression of HsTNRC6A-M2WA-siRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

HsTNRC6A-M2WA-siRNAres (TNRC6A Human)

Expression

Mammalian

Mutation

four non silent mutations A339T, R934G, D1289G, a…

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6A-CIM1mut-siRNAres_R

Plasmid

#147413

Purpose

Mammalian Expression of HsTNRC6A-CIM1mut-siRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

HsTNRC6A-CIM1mut-siRNAres (TNRC6A Human)

Expression

Mammalian

Mutation

three non silent mutations A339T, R934G, and K157…

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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psiCheck2-SV40p-mtIF3(SR)-3xFLAG-CMVp-mito-mTFP1

Plasmid

#182380

Purpose

Dual expression construct encoding shRNA-resistant mtIF3 and mito-mTFP1 from separate promoters

Depositor

Chunghun Lim

Article

Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

Inserts

mtIF3 (Mtif3 Mouse)
mito-mTFP1

Tags

3xFlag

Expression

Mammalian

Mutation

Changed CDS sequence from 'ccacgttcaagtcacga…

Promoter

CMV promoter and SV40 promoter

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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psiCheck2-SV40p-5'UTR-mtIF3(SR)-3xFLAG-3'UTR-HSVTKp-mCherry

Plasmid

#182379

Purpose

Dual expression construct encoding shRNA-resistant mtIF3 and mCherry from separate promoters

Depositor

Chunghun Lim

Article

Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

Inserts

mtIF3 (Mtif3 Mouse)
mCherry

Tags

3xFlag

Expression

Mammalian

Mutation

Changed CDS sequence from 'ccacgttcaagtcacga…

Promoter

HSV TK promoter and SV40 promoter

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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Phage-PGK-Flag-HA-FKBP-T(G177D)

Plasmid

#181735

Purpose

for lentiviral expression of brachyury ORF in mammalian cells

Depositor

Charles Lin

Article

Sheppard et al Cell Rep Med. 2021 Jan 19;2(1):100188. d

Insert

T (brachyury)

Use

Lentiviral

Mutation

a silent mutation in the PAM site of an sgRNAt ta…

Available Since

March 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pDIV495

Plasmid

#177702

Purpose

Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hansenii

Depositor

Uffe Mortensen

Article

Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

Inserts

Cas9
Nat
sgRNA Targeting ADE2 locus in D.hansenii: AGCTAaGCAGATTAAtGCAT

Use

CRISPR

Tags

SV40

Expression

Bacterial and Yeast

Promoter

RNR2p (Debaryomyces hansenii ), SRC1p (Debaryomyc…

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs7732130-guide4

Plasmid

#125447

Purpose

CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs7732130 (ZBED3 GWAS locus)

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

KRAB-dCas9-2A-BlastR

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Promoter

hPGK and U6

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs7732130-guide3

Plasmid

#125446

Purpose

CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs7732130 (ZBED3 GWAS locus)

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

KRAB-dCas9-2A-BlastR

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Promoter

hPGK and U6

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs7732130-guide2

Plasmid

#125445

Purpose

CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs7732130 (ZBED3 GWAS locus)

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

KRAB-dCas9-2A-BlastR

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Promoter

hPGK and U6

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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