We narrowed to 13,837 results for: CRISPR-Cas9

Showing: 9621 - 9640 of 13837 results

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pCAG-RNF169UBD

Plasmid

#119006

Purpose

Mammalian expression of the human RNF169 Ubiquitin binding domain and BFP

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

RNF169 (RNF169 Human)

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pUPRT::DHFR-D

Plasmid

#58528

Purpose

replace TgUPRT with DHFR by homologous recombination

Depositor

David Sibley

Article

Shen et al MBio. 2014 May 13;5(3). pii: e01114-14.

Insert

DHFR cassette

Use

Gateway cloning

Mutation

pyrimethamine resistant

Promoter

DHFR

Available Since

Aug. 6, 2014

Availability

Academic Institutions and Nonprofits only

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hRad51(K133R)

Plasmid

#125571

Purpose

Expresses the hRad51(K133R) protein in mammalian cells under CMV promoter

Depositor

David Liu

Article

Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

Insert

Rad51 (RAD51 Human)

Use

CRISPR

Expression

Mammalian

Mutation

K133R

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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AAVS1_sgRNA

Plasmid

#100554

Purpose

Expresses AAVS1 sgRNA. Target sequence: GGGGCCACTAGGGACAGGAT

Depositor

Dustin Maly

Article

Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

Insert

AAVS1 sgRNA

Promoter

U6

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-SpRY-P2A-mScarlet (MNW002)

Plasmid

#174137

Purpose

CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R); C-terminal bi-partite NLS, 3x flag tag, P2A-mScarlet

Depositor

Benjamin Kleinstiver

Insert

human codon optimized SpRY with BPNLS-3xFLAG-P2A-mScarlet

Use

In vitro transcription; t7 promoter

Tags

BPNLS-3xFLAG-P2A-mScarlet

Expression

Mammalian

Mutation

SpG=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317…

Promoter

CMV and T7

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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px458 EQR

Plasmid

#101731

Purpose

Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

SpCas9 EQR

Tags

3xFLAG-NLS and NLS

Expression

Mammalian

Mutation

EQR (D1135E, R1335Q, and T1337R mutations in Cas9)

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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LBJJ382

Plasmid

#117518

Purpose

Also known as LBJJ429. PCR template for sgRNA-EF with polyT, 67bp or 192bp terminator

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BpiI:ACTA:pU6-26:sgRNA-EF:t192bp:TTAC:BpiI

Use

CRISPR

Expression

Plant

Promoter

AtU6-26

Available Since

Oct. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pTaU6-esgRNA

Plasmid

#115630

Purpose

expression esgRNA in wheat protoplasts

Depositor

Caixia Gao

Article

Li et al Genome Biol. 2018 May 29;19(1):59. doi:

Insert

TaU6p-esgRNA

Use

CRISPR

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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SK-YFP-SPNM-B

Plasmid

#48661

Purpose

Bacterial SP and NM repression YFP reporter: protospacer B

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

SP/NM prototspacer B/PAM with reporter EYFP

Use

CRISPR

Promoter

lambda pR promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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px330 VRER

Plasmid

#101729

Purpose

Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

SpCas9 VRER

Tags

3xFLAG-NLS and NLS

Expression

Mammalian

Mutation

VRER (D1135V, G1218R, R1335E, and T1337R mutation…

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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p458 VRER

Plasmid

#101728

Purpose

Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

SpCas9 VRER

Tags

3xFLAG-NLS and NLS

Expression

Mammalian

Mutation

VRER (D1135V, G1218R, R1335E, and T1337R mutation…

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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p330 VQR

Plasmid

#101730

Purpose

Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

SpCas9 VQR

Tags

3xFLAG-NLS and NLS

Expression

Mammalian

Mutation

VQR (D1135V, R1335Q, and T1337R mutations in Cas9)

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pCfB8381

Plasmid

#126915

Purpose

Plasmid containing integration cassette at XI-3 integration site in Saccharomyces cerevisiae for overexpression of XdCrtI and tHMG1

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtI and tHMG1

Expression

Yeast

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pOsU3-esgRNA

Plasmid

#115629

Purpose

expression esgRNA in rice protoplasts

Depositor

Caixia Gao

Article

Li et al Genome Biol. 2018 May 29;19(1):59. doi:

Insert

OsU3p-esgRNA

Use

CRISPR

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pUC19, containing H2-Kb c57/BL6 WK9

Plasmid

#102641

Purpose

Encodes sequence of the murine H2-Kb allele

Depositor

Sai Reddy

Article

Kelton et al Sci Rep. 2017 Apr 4;7:45775. doi: 10.103

Insert

H2-Kd Balb/c

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pNeurog2-Luciferase reporter

Plasmid

#64126

Purpose

Photoactivatable transcription system. Luciferase reporter under the control of the Neurog2 promoter.

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

Neurog2 promoter

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

Neurog2

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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AP582-2

Plasmid

#70049

Purpose

Expresses Ollas::mCherry::linker::H2B::V5 in bacteria

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

Insert

mCherry::linker::H2B

Tags

Ollas and V5

Expression

Bacterial

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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AP588-1

Plasmid

#71278

Purpose

Expresses myc::TEV::mTagGFP::TEV::3Xflag in bacteria

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

Insert

tagGFP

Tags

TEV::3xFlag and myc::TEV

Expression

Bacterial

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-TetR-Rad18UBD

Plasmid

#119010

Purpose

Mammalian expression of a fusion protein of single chain Tet repressor with the human Rad18 Ubiquitin binding domain and BFP expression

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

TetR

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-Trex2-bpA

Plasmid

#86984

Purpose

Expression of mouse Trex2

Depositor

Ralf Kuehn

Article

Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

Insert

mouse Trex2 coding region (Trex2 Mouse)

Expression

Mammalian

Promoter

CAG

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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