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LTR-[Bxb1(attP)-spacer-BceINT(attP)-spacer-Pa01(attP)-spacer-SacINT(attP)-pEF1a-Puro-LTR (DRR728)

Plasmid

#248232

Purpose

Lentiviral plasmid containing Bxb1, BceINT, Pa01, and SacINT attP target sites

Depositor

Benjamin Kleinstiver

Article

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

LTR-[Bxb1(attP)-spacer-BceINT(attP)-spacer-Pa01(attP)-spacer-SacINT(attP)-pEF1a-Puro-LTR

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

None

Promoter

NA

Available Since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

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pGL4.10 HIF1a prom Chr5-LG4

Plasmid

#246714

Purpose

To investigate the influence of an LG4 enhancer (chr5 LG4) on HIF1a promoter activity (measured by luciferase expression)

Depositor

Glen Borchert

Article

DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

Inserts

HIF1a Promoter (HIF1A Human)
chr5 LG4 enhancer

Use

Luciferase

Mutation

WT

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pGL4.10 EXOC3prom Chr5-LG4

Plasmid

#246712

Purpose

To investigate the influence of an LG4 enhancer (chr5 LG4) on EXOC3 promoter activity (measured by luciferase expression)

Depositor

Glen Borchert

Article

DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

Inserts

EXOC3 Promoter (EXOC3 Human)
chr5 LG4 enhancer

Use

Luciferase

Mutation

WT

Available Since

Nov. 5, 2025

Availability

Academic Institutions and Nonprofits only

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cleaveBADTrp

Plasmid

#242866

Purpose

Expression of cleavable Trp1 (extracellular domain)

Depositor

Ferenc Scheeren

Article

van der Plas – van Duijn et al Commun Biol 8, 1561 (2025).

Insert

ECD Trp-1-WT fused to cleavable TMD (TYRP1 Human)

Use

Lentiviral

Tags

Cleavable CAR TMD

Expression

Mammalian

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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Trp1-WT-display

Plasmid

#242867

Purpose

Expression of ECD of WT- Trp1 fused to display TMD.

Depositor

Ferenc Scheeren

Article

van der Plas – van Duijn et al Commun Biol 8, 1561 (2025).

Insert

ECD Trp-1-WT fused to display TMD (TYRP1 Human)

Use

Lentiviral

Tags

Display domain

Expression

Mammalian

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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Trp1-Ala-display

Plasmid

#242868

Purpose

Expression of ECD of Trp-1-ala fused to display TMD.

Depositor

Ferenc Scheeren

Article

van der Plas – van Duijn et al Commun Biol 8, 1561 (2025).

Insert

Trp-1-ala (TYRP1 Human)

Use

Lentiviral

Tags

Display domain

Expression

Mammalian

Mutation

First amino acid after signal peptide mutated fro…

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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TOPO-EXOC3 (-1557 to-1046)

Plasmid

#226446

Purpose

For subcloning of human EXOC3 promoter (base pairs -1557 to-1046) or for assays using M13 phage

Depositor

Glen Borchert

Article

DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

Insert

EXOC3 (-1557 to-1046) (EXOC3 Human)

Use

Cloning vector

Mutation

No

Available Since

Sept. 15, 2025

Availability

Academic Institutions and Nonprofits only

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TOPO-EXOC3 (-2455 to -1951)

Plasmid

#226441

Purpose

For subcloning of human EXOC3 promoter (base pairs -2455 to -1951) or for assays using M13 phage

Depositor

Glen Borchert

Article

DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

Insert

EXOC3 (-2455 to -1951) (EXOC3 Human)

Use

Cloning vector

Mutation

No

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pT3TS-RfxCas13d-2C-NLS

Plasmid

#239816

Purpose

Plasmid to carry out IVT of RfxCas13d with SV40 and nucleoplasmin long-NLP NLS (human codon-optimized)

Depositor

Miguel Angel Moreno-Mateos

Article

Moreno-Sanchez et al Nat Commun. 2025 Mar 16;16(1):2591. doi:

Insert

Rfx-Cas13d

Use

CRISPR

Tags

Nucleoplasmin long NLS and SV40 NLS

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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TOPO-EXOC3 (-1592 to -1046)

Plasmid

#226444

Purpose

For subcloning of human EXOC3 promoter (base pairs -1592 to -1046) or for assays using M13 phage

Depositor

Glen Borchert

Article

DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

Insert

EXOC3 (-1592 to -1046) (EXOC3 Human)

Use

Cloning vector

Mutation

No

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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TOPO-EXOC3 (-1591 to -1009)

Plasmid

#226443

Purpose

For subcloning of human EXOC3 promoter (base pairs -1591 to -1009) or for assays using M13 phage

Depositor

Glen Borchert

Article

DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

Insert

EXOC3 (-1591 to -1009) (EXOC3 Human)

Use

Cloning vector

Mutation

No

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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TOPO-EXOC3 (-1701 to -1594)

Plasmid

#226442

Purpose

For subcloning of human EXOC3 promoter (base pairs -1701 to -1594) or for assays using M13 phage

Depositor

Glen Borchert

Article

DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

Insert

EXOC3 (-1701 to -1594) (EXOC3 Human)

Use

Cloning vector

Mutation

No

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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TOPO-EXOC3 (-1592 to -1444)

Plasmid

#226445

Purpose

For subcloning of human EXOC3 promoter (base pairs -1592 to -1444) or for assays using M13 phage

Depositor

Glen Borchert

Article

DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

Insert

EXOC3 (-1592 to -1444) (EXOC3 Human)

Use

Cloning vector

Mutation

No

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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TOPO-EXOC3 (-1557 to-1444)

Plasmid

#226447

Purpose

For subcloning of human EXOC3 promoter (base pairs -1557 to-1444) or for assays using M13 phage

Depositor

Glen Borchert

Article

DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

Insert

EXOC3 (-1557 to-1444) (EXOC3 Human)

Use

Cloning vector

Mutation

No

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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ABL1-C-HiBiT

Plasmid

#234568

Purpose

Bacterially expressed ABL1-C-HiBiT for Ni-NTA purification

Depositor

James Inglese

Article

Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:

Insert

Abelson Kinase Domain (ABL1 Human)

Tags

HiBiT Tag-thrombin site-10xHis and T7 gene 10 lea…

Expression

Bacterial

Mutation

Codon optimized for bacterial expression

Promoter

T7

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mEGFP-NONO-TFE3

Plasmid

#237641

Purpose

For overexpression of mEGFP-NONO-TFE3

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NONO-TFE3 (NONO, TFE3 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mEGFP-YAP-MAMLD1-KS

Plasmid

#237676

Purpose

For overexpression of mEGFP-YAP-MAMLD1-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

YAP-MAMLD1-KS (YAP1, MAMLD1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mEGFP-NONO-TFE3-KS

Plasmid

#237681

Purpose

For overexpression of mEGFP-NONO-TFE3-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NONO-TFE3-KS (NONO, TFE3 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mEGFP-NUP98-DDX10-Mut1

Plasmid

#237647

Purpose

For overexpression of mEGFP-NUP98-DDX10-Mut1

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NUP98-DDX10-Mut1 (NUP98, DDX10 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mEGFP-NUP98-DDX10-Mut2

Plasmid

#237648

Purpose

For overexpression of mEGFP-NUP98-DDX10-Mut2

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NUP98-DDX10-Mut2 (NUP98, DDX10 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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