We narrowed to 29,899 results for: des.2

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ABE-RA4.0

Plasmid

#208305

Purpose

Expresses ABE-RA4.0 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA4.0-SpCas9 D10A

Expression

Mammalian

Mutation

P48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pmCherry2-CD86-mEGFP

Plasmid

#190748

Purpose

Expression of CD86 double tagged with mCherry2 (N-term) and mEGFP (C-term) in mammalian cells.

Depositor

Dave Piston

Article

Foust et al bioRxiv 2021.11.03.467169

Insert

CD86 (CD86 Human)

Tags

mCherry2 and mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCD86-mEYFP(Q69K)-mEGFP

Plasmid

#190750

Purpose

Expression of CD86 double tagged with mEYFP(Q69K) and mEGFP in mammalian cells.

Depositor

Dave Piston

Article

Foust et al bioRxiv 2021.11.03.467169

Insert

CD86 (CD86 Human)

Tags

mEGFP and mEYFP(Q69K)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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Ssh1 gRNA#1

Plasmid

#163394

Purpose

Cas9-mediated knockout of Ssh1 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

ssh1 (Ssh1 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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Ssh1 gRNA#3

Plasmid

#163396

Purpose

Cas9-mediated knockout of Ssh1 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

ssh1 (Ssh1 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pT2U3-YFP

Plasmid

#84547

Purpose

Hybrid reporter construct with 5 transcription factor binding sites (2 TetO, 3 UAS) driving EYFP

Depositor

Joshua Leonard

Article

Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

Insert

T2U3-EYFP

Expression

Mammalian

Promoter

CMV-min

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pU2T4-YFP

Plasmid

#84548

Purpose

Hybrid reporter construct with 6 transcription factor binding sites (2 UAS, 4 TetO) driving EYFP

Depositor

Joshua Leonard

Article

Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

Insert

U2T4-EYFP

Expression

Mammalian

Promoter

CMV-min

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pET-Himar-dCas9-mammalian

Plasmid

#138414

Purpose

Protein purification of Himar-dCas9 with 3x-FLAG, SV40 NLS, and 6x-His tag

Depositor

Harris Wang

Article

Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

Insert

Himar-dCas9

Tags

3xFLAG, 6xHis, and SV40 NLS

Expression

Bacterial

Promoter

T7

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pJBL7007

Plasmid

#136946

Purpose

Malachite green aptamer with stability hairpin, bacterial promoter

Depositor

Julius Lucks

Article

Silverman et al ACS Synth Biol. 2019 Feb 15;8(2):403-414

Insert

malachite green aptamer (MGA)

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pJ211_WT_med

Plasmid

#128849

Purpose

Medium copy number plasmid for expression of E. coli flagellin from its natural promoter

Depositor

Mark Pallen

Article

Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

Insert

Flagellin

Expression

Bacterial

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pJ211_rha_WT_hi

Plasmid

#128853

Purpose

High copy number plasmid for expression of E. coli flagellin from a rhamnose-inducible promoter

Depositor

Mark Pallen

Article

Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

Insert

Flagellin

Expression

Bacterial

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pMM0626

Plasmid

#129001

Purpose

pRPL18B-mRUBY2 cassette plasmid flanked by ConL2 and ConRE

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConL2-pRPL18B-mRUBY2-tADH1-ConRE AmpR-ColE1

Expression

Yeast

Available Since

Oct. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0627

Plasmid

#129002

Purpose

pTDH3-mRUBY2 cassette plasmid flanked by ConL2 and ConRE

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConL2-pTDH3-mRUBY2-tADH1-ConRE AmpR-ColE1

Expression

Yeast

Available Since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0529

Plasmid

#128986

Purpose

Part plasmid for Zif268DBD-CRY2, Type 3 (coding sequence) part

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

SV40NLS-ZiF268DBD-CRY2

Use

Synthetic Biology

Available Since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0625

Plasmid

#129000

Purpose

pREV1-mRUBY2 cassette plasmid flanked by ConL2 and ConRE

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConL2-pREV1-mRUBY2-tADH1-ConRE AmpR-ColE1

Expression

Yeast

Available Since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0521

Plasmid

#128984

Purpose

Expression plasmid for ZIF268DBD-L3-CRY2

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

SV40NLS-Zif268DBD-CRY2 (L3) scTRP1

Expression

Yeast

Available Since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pVP2del50

Plasmid

#125185

Purpose

Expresses the VP2del50 truncation mutant protein derived from AAV2 VP2

Depositor

Junghae Suh

Article

Thadani et al ACS Nano. 2018 Feb 27;12(2):1445-1454. d

Insert

hexahistidine tag

Expression

Mammalian

Mutation

Inserted start codon and hexahistidine tag at 2697

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pVP2del30

Plasmid

#125184

Purpose

Expresses the VP2del30 truncation mutant protein derived from AAV2 VP2

Depositor

Junghae Suh

Article

Thadani et al ACS Nano. 2018 Feb 27;12(2):1445-1454. d

Insert

hexahistidine tag

Expression

Mammalian

Mutation

Inserted start codon and hexahistidine tag at 2637

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pClodANL-FGF2NBAA

Plasmid

#107873

Purpose

Bacterial expression of FGF2 minimum export mutant-NanoLuc

Depositor

Sanford Simon

Article

Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

Insert

FGF2-minimal export

Use

Luciferase

Tags

NanoLuc

Expression

Bacterial

Mutation

C77A, C95A, K128Q, R129Q, K134Q

Promoter

pro D

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pEPOR1CB0114

Plasmid

#117553

Purpose

Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting NbPDS gene in N. benthamiana

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

Promoter_AtU6-26 + sgRNA_NbPDS1 N. benthamiana

Expression

Plant

Promoter

At U6-26 (pICSL90002)

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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