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sankE3_5

Plasmid

#24893

Depositor

David Baker

Article

Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub

Insert

sank E3_5

Tags

His

Expression

Bacterial

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
pEcRF1H6

Plasmid

#177608

Purpose

Expresses His tagged RF1 in Escherichia coli cells

Depositor

Kambiz Hamadani

Insert

Release Factor 1

Tags

Hexahistidine

Expression

Bacterial

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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pEcRF2H6

Plasmid

#177609

Purpose

Expresses His tagged RF1 in Escherichia coli cells

Depositor

Kambiz Hamadani

Insert

Release Factor 2

Tags

Hexahistidine

Expression

Bacterial

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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pEcH6RF3

Plasmid

#177610

Purpose

Expresses His tagged RF3 in Escherichia coli cells

Depositor

Kambiz Hamadani

Insert

Release Factor 3

Tags

Hexahistidine

Expression

Bacterial

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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pEcMTFH6

Plasmid

#177601

Purpose

Expresses His tagged MTF in Escherichia coli cells

Depositor

Kambiz Hamadani

Insert

Methionyl transformylase

Tags

Hexahistidine

Expression

Bacterial

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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p304N

Plasmid

#246317

Purpose

Plant CRISPR editing with the experimentally validated Medicago truncatula MtU6.6 promoter (352 bp)

Depositor

Chung-Jui Tsai

Article

Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Oct. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA63_sgRNA-B0_M13ps

Plasmid

#231421

Purpose

M13 phagemid constitutively expressing sgRNA-B0. sgRNA-B0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI). (A gift of the Jaramillo Lab, where it is called PAJ552.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-B0

Use

Synthetic Biology

Available Since

June 9, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA63_sgRNA-B1_M13ps

Plasmid

#231422

Purpose

M13 phagemid that expresses sgRNA-B1 from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ615.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-B1

Use

Synthetic Biology

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pYO503

Plasmid

#235743

Purpose

Protein expression of KmTor by integrating ZZ-KmTOR into the K. marxianus genome

Depositor

Roger Williams

Article

Baretic et al Nat Commun. 2016 Apr 13;7:11016. doi: 10

Insert

KmTOR

Tags

ZZ-3xTEV

Expression

Yeast

Mutation

aa 4-4609

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA4TO_24xGCN4_PCC_24xPP7

Plasmid

#236094

Purpose

Constitutively encodes 24xGCN4-PCC-24xPP7 stem loops, for visualization of the Pcc mRNA.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

24xGCN4-PCC-24xPP7

Expression

Mammalian

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA4TO_24xGCN4_MUT_24xPP7

Plasmid

#236093

Purpose

Constitutively encodes 24xGCN4-MUT-24xPP7 stem loops, for visualization of the Mut mRNA.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

24xGCN4-MUT-24xPP7

Expression

Mammalian

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA19_sgRNA-C1_M13ps

Plasmid

#231411

Purpose

M13 phagemid that expresses sgRNA-C1 (= sgRNA A5T from Nielsen et al., 2014) from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ272.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-C1

Use

Synthetic Biology

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA26_sgRNA-2_M13ps

Plasmid

#231412

Purpose

M13 phagemid that expresses sgRNA-2 from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ171.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-2

Use

Synthetic Biology

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA19_sgRNA-C0_M13ps

Plasmid

#231410

Purpose

M13 phagemid constitutively expressing sgRNA-C0. sgRNA-C0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI). (A gift of the Jaramillo Lab, where it is called PAJ156.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-C0

Use

Synthetic Biology

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pVP311

Plasmid

#222070

Purpose

MultiGreen 2.0 AB level 1 acceptor vector, kanamycin selection.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Mutation

All unused Esp3i sites are domesticated

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pVP312

Plasmid

#222071

Purpose

MultiGreen 2.0 BC level 1 acceptor vector, kanamycin selection.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Mutation

All unused Esp3i sites are domesticated

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pVP313

Plasmid

#222072

Purpose

MultiGreen 2.0 CD level 1 acceptor vector, kanamycin selection.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Mutation

All unused Esp3i sites are domesticated

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pVP314

Plasmid

#222073

Purpose

MultiGreen 2.0 DE level 1 acceptor vector, kanamycin selection.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Mutation

All unused Esp3i sites are domesticated

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pVP315

Plasmid

#222074

Purpose

MultiGreen 2.0 EF level 1 acceptor vector, kanamycin selection.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Mutation

All unused Esp3i sites are domesticated

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pVP316

Plasmid

#222075

Purpose

MultiGreen 2.0 FG level 1 acceptor vector, kanamycin selection.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Mutation

All unused Esp3i sites are domesticated

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge