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hCD4-mOrange

Plasmid

#110192

Purpose

Encodes for human CD4 coding sequence tagged with the mOrange FP

Depositor

Sergi Padilla Parra

Publication

Iliopoulou et al Nat Struct Mol Biol. 2018 Sep;25(9):814-

Insert

hCD4 (CD4 Human)

Tags

mOrange

Expression

Mammalian

Promoter

CMV

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3 GATA1

Plasmid

#85693

Purpose

Mammalian expression of mouse GATA1

Depositor

Licio Collavin, Giannino Del Sal

Publication

Collavin et al Proc Natl Acad Sci U S A. 2004 Jun 15;10

Insert

GATA1 (Gata1 Mouse)

Expression

Mammalian

Promoter

CMV

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pU6-DHFR

Plasmid

#80329

Purpose

Allows for expression of an sgRNA in Toxoplasma gondii and confers resistance to pyrimethamine

Depositor

Sebastian Lourido

Publication

Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

Insert

DHFR-TSc3

Use

CRISPR

Promoter

DHFR-TS 5' UTR

Available since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pBI-MCS-EYFP

Plasmid

#58855

Purpose

The tet-responsive reporter pBI-MCS-EGFP (Addgene plasmid 16542) was modified to express YFP instead of GFP

Depositor

Joshua Leonard

Publication

Daringer et al ACS Synth Biol. 2014 Mar 11.

Insert

enhanced yellow fluorescent protein

Expression

Mammalian

Promoter

CMV

Available since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pACλCI-β-flap (831-1057)

Plasmid

#53733

Purpose

Expression of λCI fused via three alanines to E. coli RNAP beta subunit, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.

Depositor

Ann Hochschild

Publication

Dove et al Nature. 1997 Apr 10;386(6625):627-30.

Insert

λCI-β-flap fusion

Expression

Bacterial

Promoter

lac UV5

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCyPet-C1

Plasmid

#22779

Depositor

Klaus Hahn

Publication

Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

Insert

CyPet

Expression

Mammalian

Mutation

Control for intermolecular Rac1 biosensor FRET. S…

Available since

Dec. 18, 2009

Availability

Academic Institutions and Nonprofits only
Flag-Paxillin

Plasmid

#15212

Depositor

Rick Horwitz

Publication

Webb et al J Cell Sci. 2005 Nov 1. 118(Pt 21):4925-

Insert

Paxillin (PXN Chicken)

Tags

Flag

Expression

Mammalian

Available since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pcDNA GFP10-ZIP-GFP11

Plasmid

#182243

Purpose

Tripartite split-GFP mammalian. Express leucine zipper with GFP10 and GFP11 (self-assembing with GFP1-9)

Depositor

Stéphanie Cabantous

Publication

Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

Insert

GFP10-ZIP-11

Expression

Mammalian

Promoter

CMV

Available since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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SPLICS Mt-ER- PM Short P2A

Plasmid

#164110

Purpose

Detect the short Plasma membrane-mitochondria-Endoplasmic reticulum contact

Depositor

Marisa Brini, Tito Calì

Publication

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split GFP and YFP PM- Mt-ER Short P2A

Expression

Mammalian

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-Tog

Plasmid

#29480

Depositor

Linda Wordeman

Publication

Moore et al J Cell Biol. 2005 May 9. 169(3):391-7.

Insert

ch-TOG (CKAP5 Human)

Tags

EGFP

Expression

Mammalian

Mutation

K19R, E118G, G254R, K1834R

Available since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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GST HP1 gamma

Plasmid

#24076

Depositor

Naoko Tanese

Publication

Vassallo et al Proc Natl Acad Sci U S A. 2002 Apr 30. 9

Insert

HP1 gamma (CBX3 Human)

Tags

GST

Expression

Bacterial

Available since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only
YFP-PKCbetaII-YFP

Plasmid

#14866

Depositor

Alexandra Newton

Publication

Violin et al J Cell Biol. 2003 Jun 9. 161(5):899-909.

Insert

YFP-PKCbetaII-YFP

Tags

YFP

Expression

Mammalian

Available since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pMIG small T

Plasmid

#9059

Depositor

Bob Weinberg

Publication

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

SV40 small T Antigen (SV40gp7 simian virus)

Use

Retroviral

Tags

IRES GFP

Expression

Mammalian

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pMXs_FLAG-NMNAT1

Plasmid

#133259

Purpose

Express FLAG-tagged NMNAT1 in mammalian cells

Depositor

David Sabatini

Publication

Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

Insert

NMNAT1 (NMNAT1 Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Available since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pK18-hACE2

Plasmid

#149449

Purpose

Expresses human ACE2 driven by the K18 promoter

Depositor

Paul McCray

Publication

McCray PB et al J Virol. 2007 Jan;81(2):813-21. doi: 10.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Expression

Mammalian

Promoter

K18

Available since

June 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pMRE-Tn7-135

Plasmid

#118553

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mScarlet-I

Use

Minitn7 delivery vector

Expression

Bacterial

Available since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pTP813

Plasmid

#113308

Purpose

human Mre11 in baculovirus transfer vector

Depositor

Tanya Paull

Publication

Bhaskara et al Mol Cell. 2007 Mar 9;25(5):647-61. doi:

Insert

Mre11 (MRE11 Human)

Tags

Flag

Expression

Insect

Promoter

polyhedrin

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pHR-CMV-TetO2_3C-Twin-Strep

Plasmid

#113883

Purpose

Mammalian (inducible) protein expression

Depositor

A. Radu Aricescu

Publication

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Twin-Strep

Promoter

CMV-MIE-TetO2

Available since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pCMV-GCaMP6fu

Plasmid

#86235

Purpose

Express fluorescent calcium indicator GCaMP6fu in mammalian cells

Depositor

Katalin Torok

Publication

Helassa et al Sci Rep. 2016 Dec 6;6:38276. doi: 10.103

Insert

GCaMP6fu

Expression

Mammalian

Mutation

W43Y D395A

Promoter

CMV

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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MBD2a pcDNA3.1

Plasmid

#78141

Purpose

Mammalian expression of MBD2a

Depositor

Heidi Schwarzenbach

Publication

Wischnewski et al Mol Cancer Res. 2007 Jul;5(7):749-59.

Insert

MBD2a (MBD2 Human)

Expression

Mammalian

Promoter

CMV

Available since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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