165,343 results

1. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR

   Plasmid

   #118154

   Purpose

   Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Jorge Ferrer

   Insert

   KRAB-dCas9-P2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   See depositor comments below

   Promoter

   EF1a core and U6

   Available Since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRSET FlipSuc-90u delta1

   Plasmid

   #14475

   Depositor

   Wolf Frommer

   Article

   Lager et al J Biol Chem. 2006 Oct 13. 281(41):30875-

   Insert

   FlipThuE W283A
   

   Tags

   ECFP and EYFP

   Expression

   Bacterial

   Mutation

   N192D, W283A, 13 amino acids deleted from linkers

   Promoter

   T7

   Available Since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. HERV-Kcon-RT for Split-PE (pJUL3120)

   Plasmid

   #190107

   Purpose

   Expresses HERV-Kcon RT (human codon optimization)

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

   Insert

   HERV-Kcon-RT
   

   Tags

   bpNLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pT7-EGFP-C1-HsRCK

   Plasmid

   #25033

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

   Insert

   Rck (DDX6 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   June 22, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1-2xFLAG-SREBP-2

   Plasmid

   #26807

   Depositor

   Timothy Osborne

   Article

   Toth et al Mol Cell Biol. 2004 Sep . 24(18):8288-30

   Insert

   Sterol Regulatory Element Binding Protein 2 (SREBF2 Human)
   

   Tags

   2x FLAG

   Expression

   Mammalian

   Mutation

   L7V, G76S, K395R and I482L

   Promoter

   CMV

   Available Since

   July 26, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMOS023: Peredox NADH/NAD+ sensor (cytosolic)

   Plasmid

   #163060

   Purpose

   Peredox NADH/NAD+ sensor (cytosolic)

   Depositor

   Adam Cohen

   Article

   Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

   Insert

   Peredox
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. FgH1tUTG

   Plasmid

   #70183

   Purpose

   Inducible expression of guide RNA with fluorescent GFP reporter

   Depositor

   Marco Herold

   Article

   Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

   Insert

   H1-Tet-sgrna cassette
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. mCherry-CD81-10

   Plasmid

   #55012

   Purpose

   Localization: Tetraspanin/Membrane, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   CD81 (CD81 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pENTR223-BLLF1(gp350)

   Plasmid

   #195837

   Purpose

   Gateway Entry Clone of Epstein-Barr virus BLLF1

   Depositor

   Eric Johannsen

   Article

   Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

   Insert

   BLLF1 (BLLF1 Epstein-Barr virus)
   

   Use

   Gateway entry vector

   Available Since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMKO.1 GFP

    Plasmid

    #10676

    Purpose

    Retroviral shRNA vector. Derivative of pMKO.1 puro (Addgene plasmid #8452) with GFP instead of puromycin resistance gene.

    Depositor

    William Hahn

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge