We narrowed to 17,807 results for: STO;

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pAAV-hSyn-SOUL-P2A-tdTomato

Plasmid

#153019

Purpose

Step-function opsin with ultra-high light sensitivity (SOUL). Capable to be used for noninvasive stimulation in mice and minimally invasive stimulation in macaque.

Depositor

Guoping Feng

Article

Gong et al Neuron. 2020 Jul 8;107(1):197. doi: 10.1

Insert

SOUL

Use

AAV

Tags

P2A-tdTomato

Expression

Mammalian

Mutation

C128S/D156A/T159C

Available Since

June 2, 2023

Availability

Academic Institutions and Nonprofits only

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AAVS1-idCas9-KRAB-Hygro donor

Plasmid

#199621

Purpose

Donor vector for genomic targeting of a Tetracycline-inducible dCas9-KRAB cassette to the human AAVS1/PPP1R12C locus

Depositor

Danwei Huangfu

Article

Luo et al Nat Genet. 2023 Aug;55(8):1336-1346. doi

Insert

AAVS1-idCas9-KRAB-Hygro

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-SEC61B.N-BSD.P2A.miniIAA7.3xFlag

Plasmid

#216250

Purpose

HDR template to tag endogenous human SEC61B N-terminus with BSD.P2A.miniIAA7.3xFlag

Depositor

Elina Ikonen

Article

Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

Insert

HDR template for human SEC61B (SEC61B Human, Synthetic, HDR template)

Use

CRISPR; Hdr template

Tags

BSD.P2A.miniIAA7.3xFlag

Expression

Mammalian

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pF CAG luc IRES neo

Plasmid

#98294

Purpose

For constitutive expression of firefly luciferase from the CAG promoter together with an IRES-linked neo resistance gene

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Inserts

Firefly luciferase
IRES
Neomycin resistance gene (NeoR)

Use

Lentiviral and Luciferase

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-hVAP-A KD/MD

Plasmid

#104449

Purpose

Expression of human VAP-A (mutant K94D/M96D, unable to bind FFAT motifs) fused to GFP in mammalian cells

Depositor

Catherine Tomasetto

Article

Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

Insert

vesicle-associated membrane protein-associated protein A (VAPA Human)

Tags

EGFP

Expression

Mammalian

Mutation

changed K94 to D and M96 to D

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-eGFP-msMMS22L

Plasmid

#221014

Purpose

mammalian expression vector of eGFP tagged mouse MMS22L

Depositor

Daniel Durocher

Article

O'Donnell et al Mol Cell. 2010 Nov 24;40(4):619-31. doi:

Insert

MMS22L (Mms22l Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

naturally siResistant to siMMS22L (human) aagacuu…

Promoter

CMV

Available Since

Sept. 5, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pUC19-U6-BsmBI_epegRNA_entry-mpknot_(LM1140)

Plasmid

#228253

Purpose

pU6 entry plamid for cloning prime editor mpknot epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

mpknot entry plasmid backbone for epegRNA expression via human U6 promoter

Expression

Mammalian

Mutation

n/a

Promoter

U6

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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G9A[SET]-dCas9

Plasmid

#100089

Purpose

Catalytic domain [SET] of human G9A fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

Depositor

David Segal

Article

O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

Insert

G9A (EHMT2 Human)

Use

CRISPR

Tags

3XFLag-NLS-G9A[SET]-dCas9-NLS

Expression

Mammalian

Mutation

G9A[SET] includes aa 829-1209

Promoter

CMV

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pMSCV-hygro-STING HAQ+V155M

Plasmid

#102601

Purpose

Express STING V55M HAQ for IFN-beta Luciferase reporter assay.

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

V155M R71H-G230A-R293Q

Available Since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pET21-10XHis-GST-HRV-dL5

Plasmid

#73214

Purpose

Bacterial expression vector for dL5(E52D) protein with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP)

Depositor

Marcel Bruchez

Article

Wang et al Bioconjug Chem. 2015 Jan 21;26(1):137-44

Insert

EGFR (EGFR Human, Schistosoma japonicum)

Tags

10XHis-GST-HRV

Expression

Bacterial

Promoter

T7

Available Since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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pWZL-Blast FAP S624A

Plasmid

#207402

Purpose

Expresses the S624A mutant of FAP (Fibroblast Activation Protein), which shows no protease activity.

Depositor

Jing Yang

Article

Bukhari et al Cell Rep. 2023 Oct 31;42(10):113302. doi

Insert

Fibroblast Activation Protein Alpha (FAP Human)

Use

Retroviral

Mutation

changed Serine 624 to Alanine. It's a protea…

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pWZL-Blast FAP A657S

Plasmid

#207403

Purpose

Expresses the A657S mutant of FAP (Fibroblast Activation Protein), which shows partial protease activity.

Depositor

Jing Yang

Article

Bukhari et al Cell Rep. 2023 Oct 31;42(10):113302. doi

Insert

Fibroblast Activation Protein Alpha (FAP Human)

Use

Retroviral

Mutation

changed Alanine 657 to Serine. It's an endop…

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-H3.3(K36M)-3AID-HA-2A-mCherryBSD

Plasmid

#225699

Purpose

Lentiviral expression of AID degron tagged H3.3(K36M) and mCherry fused BlasticidinR

Depositor

Lacra Bintu

Article

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Insert

H3.3 (K36M) (H3-3A, H3-3B Human)

Use

Lentiviral and Synthetic Biology

Tags

3xAID HA and T2A-mCherry

Expression

Mammalian

Mutation

K36M

Promoter

pEF1A

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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rag2:myr-Akt2_pISceI

Plasmid

#231495

Purpose

Expresses myristolated Akt2 in zebrafish lymphoid and mesenchymal cells

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

Akt2 (Akt2 Mouse)

Use

Zebrafish expression

Tags

N-terminal myristolation signal

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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LifeAct-EGFP-APEX2 in pCSDEST2

Plasmid

#170523

Purpose

Vector for expression of LifeAct APEX2 in mammalian cells. Parton lab clone FTE

Depositor

Rob Parton

Article

Rae et al Elife. 2021 Apr 27;10. pii: 64630. doi:

Insert

Cavin4 (Cavin4 Mouse)

Tags

APEX2-P2A-mKate2

Expression

Mammalian

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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ITGB1

Plasmid

#51920

Purpose

Expresses full-length Integrin beta-1 precursor ectodomain in mammalian cells. Stop codon before C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

ITGB1 (ITGB1 Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Mutation

Stop Codon at amino acid 728, before CD4, bio and…

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pBFC0619

Plasmid

#186690

Purpose

VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, GmR+sfGFP cargo

Depositor

Jennifer Doudna

Article

Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

Inserts

tnsA
tnsB
tnsC
tnsD
tniQ
cas8-cas5 fusion
cas7
cas6
Vc_2xBsaI_NT (Guide Stuffer) crRNA
Tn7 transposon
GmR+sfGFP VcDART Cargo

Expression

Bacterial

Promoter

PLac

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pET30-2-PSAT1

Plasmid

#83908

Purpose

stable overexpression

Depositor

David Sabatini

Article

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

phosphoserine aminotransferase 1 (PSAT1 Human)

Tags

6x His tags

Expression

Bacterial

Promoter

T7

Available Since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pET-26b-Nb127D01-ALFA-His

Plasmid

#171568

Purpose

Bacterial expression of ALFA-His-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-ALFA-His)

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

Nb127D01-ALFA-His (CXCR2 Human)

Tags

ALFA-tag/His-tag and PelB signal peptide

Expression

Bacterial

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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ITGB3

Plasmid

#51921

Purpose

Expresses full-length Integrin beta-3 precursor ectodomain in mammalian cells. Stop codon before C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

ITGB3 (ITGB3 Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Mutation

Stop Codon at amino acid 718, before CD4, bio and…

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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