We narrowed to 31,956 results for: Eng

1. mCherry-PIS

   Plasmid

   #119078

   Purpose

   Visualize localization of ER enzyme Phosphatidylinositol Synthase (PIS)

   Depositor

   Gia Voeltz

   Article

   English et al Nat Cell Biol. 2013 Feb;15(2):169-78. do

   Insert

   PIS (CDIPT Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. Sg-A

   Plasmid

   #83807

   Purpose

   Express sgRNA targeting SA site

   Depositor

   Bo Feng

   Article

   He et al Nucleic Acids Res. 2016 May 19;44(9):e85

   Insert

   SgRNA
   

   Use

   CRISPR

   Available Since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSLS.492

   Plasmid

   #184957

   Purpose

   Test effect of a1/a2 length on editing rpoB using retron recombineering

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 22
   

   Expression

   Bacterial

   Mutation

   rpoB donor T1534C, a1/a2 length extended to 22 bp

   Promoter

   T7/lac

   Available Since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQAT39

   Plasmid

   #223411

   Purpose

   T-DNA vector for SpRY-D10A based A-to-G base editing for dicot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants select.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   2x35s-ecTadA8e-SpRY-D10A-AtU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. sgRNA 2 GAPDH

   Plasmid

   #83809

   Purpose

   Express Sg-2 sgRNA targeting GAPDH

   Depositor

   Bo Feng

   Article

   He et al Nucleic Acids Res. 2016 May 19;44(9):e85

   Insert

   SgRNA
   

   Use

   CRISPR

   Available Since

   Dec. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTrex-n-eGFP

   Plasmid

   #62544

   Purpose

   Expression of eGFP in T. cruzi. The NheI-linearized form of this plasmid could be efficiently integrated in to T. cruzi genome

   Depositor

   Rick Tarleton

   Article

   Peng et al MBio. 2014 Dec 30;6(1). pii: e02097-14.

   Insert

   eGFP
   

   Use

   (1)trypanosoma cruzi expression of egfp (2) genom…

   Tags

   None

   Mutation

   None

   Promoter

   Ribo-HX1

   Available Since

   April 16, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pYPQAT45

   Plasmid

   #223417

   Purpose

   T-DNA vector for temperature tolerance LbCas12a-D156R based mutagenesis for dicot plants; TTTV PAM; LbCas12a-D156R and the crRNA was driven by separate 2x35s promoter; Kanamycin for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   2x35s-LbCas12a-D156R-2x35s-crRNA scaffold
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Plant

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. sgRNA 1 GAPDH

   Plasmid

   #83808

   Purpose

   Express Sg-1 sgRNA targeting GAPDH

   Depositor

   Bo Feng

   Article

   He et al Nucleic Acids Res. 2016 May 19;44(9):e85

   Insert

   SgRNA
   

   Use

   CRISPR

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP_sfCherry2(11)_HP1

   Plasmid

   #82607

   Purpose

   Expresses sfCherry2(11) tagged heterochromatin protein1 in mammalian cells

   Depositor

   Bo Huang

   Article

   Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

   Insert

   sfCherry2(11)_HP1b
   

   Expression

   Mammalian

   Mutation

   changed Gly12 to Ala in sfCherry11+

   Available Since

   June 29, 2017

   Availability

   Academic Institutions and Nonprofits only

10. pEB-p53shRNA

    Plasmid

    #28222

    Depositor

    Linzhao Cheng

    Article

    Chou et al Cell Res. 2011 Mar;21(3):518-29. doi: 10

    Insert

    p53 shRNA
    

    Expression

    Mammalian

    Available Since

    March 25, 2011

    Availability

    Academic Institutions and Nonprofits only