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pDONR223_GATA3_WT

Plasmid

#81902

Purpose

Gateway Donor vector containing GATA3 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

GATA3 (GATA3 Human)

Use

Gateway entry vector

Mutation

V3A

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCDH-3_Flag-YTHDF2-m6A-MUT

Plasmid

#238976

Purpose

Expresses the m6A-binding-deficient mutant of YTHDF2

Depositor

Jianjun Chen

Article

Chen et al Cell. 2024 Dec 12:S0092-8674(24)01324-2.

Insert

YTHDF2-m6A-MUT (YTHDF2 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

2 site mutation (W432A+W486A)

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR223_RELA_WT

Plasmid

#82160

Purpose

Gateway Donor vector containing RELA , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RELA (RELA Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pmiRA1-MYC

Plasmid

#141121

Purpose

Lentiviral overexpression of MYC in mammalian cells.

Depositor

Jianjun Chen

Article

Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

Insert

MYC (MYC Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_DVL1_WT

Plasmid

#82110

Purpose

Gateway Donor vector containing DVL1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

DVL1 (DVL1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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p11d-tRPA-32Asp8

Plasmid

#102617

Purpose

Expression of human RPA with aspartic acid substituted phosphorylation domain

Depositor

Marc Wold

Article

Binz et al J Biol Chem. 2003 Sep 12;278(37):35584-9

Inserts

RPA1 (RPA1 Human)
RPA3 (RPA3 )
RPA2 (RPA2 )

Expression

Bacterial

Mutation

with aspartic acid substituted phosphorylation do…

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ATF6_WT

Plasmid

#82098

Purpose

Gateway Donor vector containing ATF6 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ATF6 (ATF6 Human)

Use

Gateway entry vector

Mutation

M67L; A145P; P157S; M313I

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_JUN_WT

Plasmid

#82138

Purpose

Gateway Donor vector containing JUN , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

JUN (JUN Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag PFKM

Plasmid

#20568

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PFKM (PFKM Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pBactin-PACT-mOrange2-hCM1

Plasmid

#196868

Purpose

Expression of Pericentrin-AKAP450 centrosomal targeting (PACT) domain fused to mOrange2 and human (h) Centrosomin motif 1 (CM1). PACT targets CM1 to the centrosome

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

PACT-mOrange2-hCM1 (Akap9 Rat)

Expression

Mammalian

Promoter

Chicken bactin (plus Chicken bactin intron)

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-1BPNLS-Cas9-1BPNLS

Plasmid

#87108

Purpose

BPNLS-Cas9-BPNLS expression plasmid

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

1BPNLS-Cas9-1BPNLS

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CAG

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RB1_WT

Plasmid

#82275

Purpose

Gateway Donor vector containing RB1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RB1 (RB1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RB1_WT

Plasmid

#81857

Purpose

Gateway Donor vector containing RB1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RB1 (RB1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-SpyTag-Flag-Lbr-V5-mCherry

Plasmid

#235094

Purpose

Lbr mCherry fusion protein without MCP domain

Depositor

Mitchell Guttman

Article

Bhat et al Nature. 2024 May;629(8014):1165-1173. do

Insert

pCAG-SpyTag-Flag-Lbr-V5-mCherry (LBR Human)

Expression

Mammalian

Promoter

CAG

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag RPSK6A3

Plasmid

#20627

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

RPSK6A3 (RPS6KA3 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pDONR223_SMAD2_WT

Plasmid

#81830

Purpose

Gateway Donor vector containing SMAD2 ,part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SMAD2 (SMAD2 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pSLED.NPL

Plasmid

#193014

Purpose

Neuron-specific reporter (CBh promoter, Pls3 exon, bichromatic reporter)

Depositor

Jonathan Ling

Article

Ling et al Nat Commun. 2022 Oct 1;13(1):5773. doi:

Insert

Bichromatic reporter (EGFP and dsRed-Express2)

Use

AAV

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pBactin-AcGFP-128-425-Akap9

Plasmid

#196871

Purpose

Expression of fragment 128-425 from A-Kinase Anchoring Protein 9 (Akap9) fused to AcGFP. Used to displace endogenous Akap9 from Golgi

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

AcGFP-Akap9 (128-425) (Akap9 Rat)

Expression

Mammalian

Promoter

Chicken bactin (plus Chicken bactin intron)

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223_BCL6_WT

Plasmid

#81869

Purpose

Gateway Donor vector containing BCL6 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

BCL6 (BCL6 Human)

Use

Gateway entry vector

Mutation

R160C

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_C15orf23_WT

Plasmid

#81896

Purpose

Gateway Donor vector containing C15ORF23 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

C15ORF23 (KNSTRN Human)

Use

Gateway entry vector

Mutation

A40E; R75L; P92S; S232G

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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