We narrowed to 21,628 results for: ESC;

1. pT/Caggs-NRAS G12V/D38A

   Plasmid

   #221073

   Purpose

   Expresses NRAS G12V/D38A in mammalian cells

   Depositor

   Hidenori Ichijo

   Article

   Odawara et al Commun Biol. 2024 Jun 5;7(1):691. doi: 1

   Insert

   NRAS (NRAS Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   G12V/D38A

   Promoter

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX6P1-mClover3-anti-rabbit-IgG(Fc)-nanobody

   Plasmid

   #138129

   Purpose

   Expresses GST-tagged mClover3-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

   Insert

   mClover3-anti-rabbit-IgG(Fc)-nanobody
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX6P1-mCherry-anti-rabbit-IgG(Fc)-nanobody

   Plasmid

   #138131

   Purpose

   Expresses GST-tagged mCherry-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

   Insert

   mCherry-anti-rabbit-IgG(Fc)-nanobody
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. plenti-CMV-mCherry-T2A-GFP

   Plasmid

   #109427

   Purpose

   A lentiviral backbone expressing mCherry-T2A-eGFP off of a CMV promoter.

   Depositor

   Reuben Harris

   Article

   St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

   Insert

   mCherry T2A GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   July 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. mEmerald-LaminB1-10

   Plasmid

   #54140

   Purpose

   Localization: Nuclear Envelope, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Article

   Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

   Insert

   LaminB1 (LMNB1 Human)
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 10, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pcDNA3.1 His-V5 Myc

   Plasmid

   #176049

   Purpose

   Expresses Myc tagged with His and V5 in Mammalian cells

   Depositor

   Linda Penn

   Article

   Tu et al Cancer Cell. 2018 Oct 8;34(4):579-595.e8

   Insert

   Myc
   

   Use

   Tags

   6xHis and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV.hSynapsin.(cyto).iATPSnFR2.A95A.A119L.HaloTag

   Plasmid

   #209665

   Purpose

   Expression of iATPSnFR2, low affinity

   Depositor

   Tim Brown, HHMI-JRC Tool Translation Team

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   iATPSnFR2.A95A.A119L.HaloTag
   

   Use

   AAV

   Tags

   Expression

   Mutation

   A95A.A119L

   Promoter

   hSyn

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. mTagRFP-T-H2B-6

   Plasmid

   #58019

   Purpose

   Localization: Nucleus/Histones, Excitation: 555, Emission: 584

   Depositor

   Michael Davidson

   Article

   Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

   Insert

   H2B (H2BC11 Human)
   

   Use

   Tags

   mTagRFP

   Expression

   Mammalian

   Mutation

   D26G and V119I in H2B

   Promoter

   CMV

   Available since

   July 31, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. Str-KDEL_ST-SBP-mCherry

   Plasmid

   #65265

   Purpose

   synchronize trafficking of ST from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Str-KDEL and truncated sialyltransferase (ST6GAL1 Human)
   

   Use

   Tags

   Streptavidin Binding Protein (SBP) and mCherry

   Expression

   Mammalian

   Mutation

   contains only amino acids 1 to 43 of ST

   Promoter

   CMV

   Available since

   June 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA5FRT/TO_HA-mAID-nanobody

    Plasmid

    #117713

    Purpose

    Expression of tetracycline-inducible HA-tagged lysine-less mAID fused to lysine-less vhhGFP4 (mAID-nanobody)

    Depositor

    Joerg Mansfeld

    Article

    Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

    Insert

    mAID-nanobody
    

    Use

    Tags

    triple HA

    Expression

    Mammalian

    Mutation

    codon optimized mAID for murine expression

    Promoter

    CMV

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pINDUCER13 miR-200c-141

    Plasmid

    #81020

    Purpose

    Lentiviral vector containing doxycycline inducible human miR-200c. Puromycin selectable.

    Depositor

    Jeffrey Rosen, Thomas Westbrook

    Article

    Toneff et al BMC Biol. 2016 Jun 17;14(1):47.

    Insert

    miR-200c (MIR200C Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTS2395-Tier1-PhCMV-Igk_mRuby2_KDEL

    Plasmid

    #169533

    Purpose

    Tier-1 vector encoding PhCMV-driven red fluorescent protein mRuby2 localized to the ER (PhCMV-Igk-mRuby2-KDEL-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven ER-localized monomeric red fluorescent protein mRuby2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. MMLV-CAG-TVA-IRES-Puro

    Plasmid

    #172366

    Purpose

    Generation of TVA-expressing stable cell lines, resistant to the antibiotic Puromycin

    Depositor

    Peter Jonas, Yoav Ben-Simon

    Article

    Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

    Insert

    TVA + Puro
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBSV2G_P0826-BB0323-mCherryBb

    Plasmid

    #118244

    Purpose

    Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes a BB0323-mCherry translational fusion under the control of the bb0826 promoter

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

    Insert

    BB0323-mCherry translational fusion under the control of the bb0826 promoter
    

    Use

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pREDIA

    Plasmid

    #51625

    Purpose

    E. coli genome modification

    Depositor

    Sheng Yang

    Article

    Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

    Insert

    I-SceI (SCEI Budding Yeast)
    

    Use

    Red recombinase and i-scei endonuclease

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pRha

    Available since

    May 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV_NFL(WT)-FLAG

    Plasmid

    #182654

    Purpose

    Expresses wild-type mouse neurofilament light chain with a C-terminal FLAG tag (DYKDDDDK) in mammalian cells.

    Depositor

    Ivana Nikić-Spiegel

    Article

    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

    Insert

    wild type mouse neurofilament light chain (Nefl Mouse)
    

    Use

    Tags

    FLAG tag (DYKDDDDK)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti_Blast_DEST mouse_p65promoter_mouse_p65-mRuby2

    Plasmid

    #124903

    Purpose

    Lentiviral vector to express p65-mRuby2 under p65 promoter (With blasticidin resistance)

    Depositor

    Markus Covert

    Article

    Lane et al Cell Syst. 2019 Mar 27;8(3):183-196.e4.

    Insert

    p65 promoter- p65 CDS-mRuby2
    

    Use

    Lentiviral

    Tags

    mRuby

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDIV298

    Plasmid

    #204957

    Purpose

    AMA1 plasmid with Aspergillus optimized Mad7 and pyrG selection marker

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

    Inserts

    Mad7
    pyrG
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    Aspergillus nidulans tef1 promoter and native pro…

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFX-β-tubulin-EGFP

    Plasmid

    #175827

    Purpose

    Express EGFP with microtubule-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    EGFP with microtuble-targeting sequence
    

    Use

    Tags

    human tubulin beta 4B class IVb;TUBB4B

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. PH-PLCD1_mScarlet_IRES_SYFP2_PH_N1

    Plasmid

    #85069

    Purpose

    Yellow/red FRET biosensor for monotoring plasmamembrane PtdIns(4,5)P2 levels (PIP2)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    PH-PLCD1 (PLCD1 Human)
    

    Use

    Tags

    IRES-sYFP2-PH and mScarlet

    Expression

    Mammalian

    Mutation

    PLCD aa 1–170

    Promoter

    CMV

    Available since

    Oct. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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