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p-mCherry-C1-Phobos

Plasmid

#98166

Purpose

blue-shifted artificial anion conducting channelrhodopsin (aACR). Activation max 466 nm; off-kinetics 10 ms. Codon optimized for mammalian expression.

Depositor

Peter Hegemann

Article

Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

Insert

Synthetic construct Phobos gene

Tags

mCherry

Expression

Mammalian

Mutation

T59S, E83N, E90Q, E101S, V117R, E123S, T159G, G16…

Promoter

CMV (+enhancer)

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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p-miR-216a

Plasmid

#65054

Purpose

Expression of miR216a in mammalian cells

Depositor

Cristina Fillat

Article

Bofill-De Ros et al Mol Ther. 2014 Sep;22(9):1665-77. doi: 1

Insert

hsa-miR-216a (pre-miRNA) (MIR216A Human)

Use

Retroviral

Expression

Mammalian

Promoter

SV40

Available Since

June 10, 2015

Availability

Academic Institutions and Nonprofits only

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pSA3

Plasmid

#52263

Purpose

Bacterial expression of human 6His-tagged SUMO3 and SUMO-E1

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Inserts

SUMO-3 (SUMO3 Human)
SAE-2 (UBA2 Human)
SAE-1 (SAE1 Human)

Tags

6His-tag

Expression

Bacterial

Mutation

C-terminal gly-gly

Promoter

T7 and rbs

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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PLK3 1-334-pEGFP

Plasmid

#23266

Depositor

Wei Dai

Article

Yang et al Cancer Res. 2008 Jun 1. 68(11):4077-85.

Insert

PLK3 (PLK3 Human)

Tags

pEGFP

Expression

Mammalian

Mutation

aa 1-334

Available Since

Dec. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pGL-p21UTRm1

Plasmid

#20878

Depositor

Robert Blelloch

Article

Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

Insert

p21 3'UTR site 1 mutation (Cdkn1a Mouse)

Use

Luciferase

Expression

Mammalian

Mutation

Predicted miRNA binding site 1 (Position ~420) GC…

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only
pCAG-hHDAC4

Plasmid

#21096

Depositor

Connie Cepko

Article

Chen et al Science. 2009 Jan 9. 323(5911):256-9.

Insert

Histone Deacetylase 4 (HDAC4 Human)

Expression

Mammalian

Mutation

shRNA target sequence was mutated (mutated nucleo…

Promoter

CAG

Available Since

May 5, 2009

Availability

Academic Institutions and Nonprofits only
XE254 pCDNA3.1(zeo)-hDsh3-497-716

Plasmid

#16755

Depositor

Randall Moon

Article

Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

Insert

FLAG-497-716-Dsh3 (DVL3 Human)

Tags

flag

Expression

Mammalian

Mutation

aa 497-716

Available Since

March 14, 2008

Availability

Academic Institutions and Nonprofits only

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LB1376 (Bcl-2 P1 promoter with MHS1234)

Plasmid

#15387

Depositor

Linda Boxer

Article

Duan et al Oncogene. 2007 Apr 19. 26(18):2635-41.

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial and Mammalian

Mutation

Bcl-2 P1 promoter with IgH full enhancer MHS1234

Available Since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only

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L1-1180

Plasmid

#13265

Depositor

Vance Lemmon

Article

Cheng et al J Neurosci. 2005 Jan 12. 25(2):395-403.

Insert

L1CAM (L1CAM Human)

Expression

Bacterial

Mutation

Truncated after RSLE region

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
L1dRSLE

Plasmid

#13266

Depositor

Vance Lemmon

Article

Cheng et al J Neurosci. 2005 Jan 12. 25(2):395-403.

Insert

L1CAM (L1CAM Human)

Expression

Bacterial

Mutation

RSLE region removed

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
815 pSG5L HA PTEN delta274-342

Plasmid

#10738

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

PTEN (PTEN Human)

Tags

HA

Expression

Mammalian

Mutation

Deletion of amino acids 274-342.

Available Since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only
p16 nlsGPS2

Plasmid

#228093

Purpose

Expression plasmid for genetically-encoded FRET biosensor for Gibberellin (GA)

Depositor

Alexander Jones

Article

Griffiths et al Plant Cell. 2024 Jul 23:koae198. doi: 10

Insert

nlsGPS2

Tags

6xHis-T7 XpressTag and cMyc

Expression

Plant

Promoter

p16

Available Since

April 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+FLAG-KrasnatG12D

Plasmid

#206842

Purpose

To express mouse Kras encoded by native mouse codons and with a G12D mutation with an N-terminal FLAG tag.

Depositor

Christopher Counter

Article

Le Roux et al Elife. 2022 Sep 7;11:e75715. doi: 10.755

Insert

Kras (Kras Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

changed Glycine 12 to Aspartic Acid

Available Since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-VIP [L108/92R]

Plasmid

#177453

Purpose

Mammalian Expression Plasmid of anti-VIP (Human). Derived from hybridoma L108/92.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-VIP (Homo sapiens) recombinant mouse monoclonal antibody (VIP Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+-Pard3-EGFP-PIF6

Plasmid

#154914

Purpose

Full length zebrafish Pard3 (Gene ID: 403050), tagged with EGFP fluorescent protein and Arabidopsis PIF6 (as in construct Addgene ID 154913).

Depositor

Clare Buckley, Jonathan Clarke

Article

Buckley et al Dev Cell. 2016 Jan 11;36(1):117-26. doi:

Insert

par-3 family cell polarity regulator alpha, b (pard3ab Zebrafish)

Use

Xenopus/avian/zebrafish

Tags

1-100 amino acids of Arabidopsis PIF6 and EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Nprot-HF_GC3opt

Plasmid

#157732

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 N protein under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nprot-HF_GC3opt (N SARS-CoV-2)

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized; gene expression was optimized by…

Available Since

Aug. 10, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1 Mfn2-HA S27A

Plasmid

#139193

Purpose

Expresses C-terminal HA tagged human Mfn2 S27A in mammalian cells

Depositor

Allan Weissman

Article

Leboucher et al Mol Cell. 2012 Aug 24;47(4):547-57. doi:

Insert

MFN2 (MFN2 Human)

Tags

3HA

Expression

Mammalian

Mutation

Serine 27 to Alanine

Promoter

CMV promoter

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pCCLc-U6-shHMGA2.3-PGK-dTomato

Plasmid

#89606

Purpose

Silencing Hmga2 in human cells

Depositor

Fernando Fierro

Article

Kalomoiris et al J Cell Biochem. 2016 Sep;117(9):2128-37.

Insert

shRNA targeting HMGA2 (HMGA2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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pBabe-PI(WT)-Rac1(WT)-mCherry

Plasmid

#87550

Purpose

PI(WT): Photo-inhibitable/blue light sensitive Rac1

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
Rac1 (RAC1 Human)

Tags

mCherry

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ALK6 K231R

Plasmid

#80884

Purpose

mammalian expression of ALK6 K231R

Depositor

Aristidis Moustakas

Article

Raja et al Oncotarget. 2016 Jan 12;7(2):1120-43. do

Insert

ALK6 (Bmpr1b Mouse)

Tags

HA

Expression

Mammalian

Mutation

K231R (kinase-deficient)

Promoter

CMV

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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